{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T23:29:57Z","timestamp":1784417397843,"version":"3.55.0"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T00:00:00Z","timestamp":1548028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Electronic cigarette (e-cigarette) vapor comes in contact with the different constituents of the oral cavity, including such microorganisms as Candida albicans. We examined the impact of e-cigarettes on C. albicans growth and expression of different virulent genes, such as secreted aspartic proteases (SAPs), and the effect of e-cigarette vapor-exposed C. albicans on gingival epithelial cell morphology, growth, and lactate dehydrogenase (LDH) activity. An increase in C. albicans growth was observed with nicotine-rich e-cigarettes compared with non-exposed cultures. Following exposure to e-cigarette vapor, C. albicans produced high levels of chitin. E-cigarettes also increased C. albicans hyphal length and the expression of SAP2, SAP3, and SAP9 genes. When in contact with gingival epithelial cells, e-cigarette-exposed C. albicans adhered better to epithelial cells than the control. Indirect contact between e-cigarette-exposed C. albicans and gingival epithelial cells led to epithelial cell differentiation, reduced cell growth, and increased LDH activity. Overall, results indicate that e-cigarettes may interact with C. albicans to promote their pathogenesis, which may increase the risk of oral candidiasis in e-cigarette users.<\/jats:p>","DOI":"10.3390\/ijerph16020294","type":"journal-article","created":{"date-parts":[[2019,1,22]],"date-time":"2019-01-22T03:08:22Z","timestamp":1548126502000},"page":"294","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["E-Cigarettes Increase Candida albicans Growth and Modulate its Interaction with Gingival Epithelial Cells"],"prefix":"10.3390","volume":"16","author":[{"given":"Humidah","family":"Alanazi","sequence":"first","affiliation":[{"name":"Groupe de Recherche en \u00c9cologie Buccale, Facult\u00e9 de M\u00e9decine Dentaire, Universit\u00e9 Laval, 2420 de la Terrasse, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1643-0377","authenticated-orcid":false,"given":"Abdelhabib","family":"Semlali","sequence":"additional","affiliation":[{"name":"Groupe de Recherche en \u00c9cologie Buccale, Facult\u00e9 de M\u00e9decine Dentaire, Universit\u00e9 Laval, 2420 de la Terrasse, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Witold","family":"Chmielewski","sequence":"additional","affiliation":[{"name":"Groupe de Recherche en \u00c9cologie Buccale, Facult\u00e9 de M\u00e9decine Dentaire, Universit\u00e9 Laval, 2420 de la Terrasse, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Rouabhia","sequence":"additional","affiliation":[{"name":"Groupe de Recherche en \u00c9cologie Buccale, Facult\u00e9 de M\u00e9decine Dentaire, Universit\u00e9 Laval, 2420 de la Terrasse, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jinf.2013.05.004","article-title":"Cigarette smoking and mechanisms of susceptibility to infections of the respiratory tract and other organ systems","volume":"67","author":"Feldman","year":"2013","journal-title":"J. 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