{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:45:18Z","timestamp":1789429518452,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T00:00:00Z","timestamp":1550620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>We analyzed the effect of diosgenin, administered with atorvastatin or ezetimibe, on the fate of 3H(G)-taurocholic acid or 26-14C-cholesterol in hypercholesterolemic rats. Male Wistar rats received a hypercholesterolemic diet (HD), HD + atorvastatin (HD+ATV), HD + ezetimibe (HD+EZT), HD + diosgenin (HD+DG), HD+ATV+EZT, or HD+ATV+DG for 40 days. We also included a control normal group (ND). The labelled compounds were administered on day 30. The animals were placed in metabolic cages for daily feces collection. At day 40 the rats were sacrificed. Lipid extracts from blood, liver, spinal cord, testicles, kidneys, epididymis, intestine, and feces were analyzed for radioactivity. Cholesterol activity was the highest in the liver in HD rats. DG diminished one half of this activity in HD+DG and HD+ATV+DG groups in comparison with the HD group. HD+ATV rats showed four to almost ten-fold cholesterol activity in the spinal cord compared with the ND or HD rats. Fecal elimination of neutral steroids was approximately two-fold higher in the HD+DG and HD+ATV+DG groups. Taurocholic acid activity was four to ten-fold higher in HD+DG intestine as compared to the other experimental groups. Taurocholic activity in the liver of HD and HD+DG groups was two and a half higher than in ND. Our results show that the combination of DG and ATV induced the highest cholesterol reduction in the liver and other tissues.<\/jats:p>","DOI":"10.3390\/ijerph16040627","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T03:49:44Z","timestamp":1550807384000},"page":"627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Combined Effect of Diosgenin Along with Ezetimibe or Atorvastatin on the Fate of Labelled Bile Acid and Cholesterol in Hypercholesterolemic Rats"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6099-5650","authenticated-orcid":false,"given":"Alejandro","family":"Mar\u00edn-Medina","sequence":"first","affiliation":[{"name":"Divisi\u00f3n Acad\u00e9mica de Ciencias de la Salud, Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico"},{"name":"Universidad de Guadalajara, CUCS, Guadalajara C.P. 44340, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Ru\u00edz-Hidalgo","sequence":"additional","affiliation":[{"name":"Hospital Angeles de Villahermosa, Villahermosa C.P. 86035, Tabasco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1376-1461","authenticated-orcid":false,"given":"Jorge L.","family":"Bl\u00e9-Castillo","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Acad\u00e9mica de Ciencias de la Salud, Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma M.","family":"Zetina-Esquivel","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Acad\u00e9mica de Ciencias de la Salud, Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Miranda","family":"Zamora","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Divisi\u00f3n de Investigaci\u00f3n, Departamento de Bioqu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad Universitaria, Ciudad de M\u00e9xico C.P. 04510, Ciudad de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7405-3242","authenticated-orcid":false,"given":"Isela E.","family":"Ju\u00e1rez-Rojop","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n Acad\u00e9mica de Ciencias de la Salud, Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"D\u00edaz-Zagoya","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Divisi\u00f3n de Investigaci\u00f3n, Departamento de Bioqu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad Universitaria, Ciudad de M\u00e9xico C.P. 04510, Ciudad de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.2147\/VHRM.S29010","article-title":"Patient considerations and clinical impact of cholesteryl ester transfer protein inhibitors in the management of dyslipidemia: Focus on anacetrapib","volume":"8","author":"Miyares","year":"2012","journal-title":"Vasc. 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