{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T05:46:40Z","timestamp":1762408000906,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T00:00:00Z","timestamp":1599177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Phytoecdysteroids are natural compounds with therapeutic benefits in both humans and animals. The effectiveness of natural products with health potential is based on the activities and potencies of their active ingredients. In this study, dominant ecdysteroids\u2014ajugasterone C, 20-hydroxyecdysone and polypodine B\u2014from the Serratula coronata (S. coronata) herb were separated by column chromatography, identified by spectroscopic data and quantified by high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained concentration of ecdysteroids (approximately 23%) in the S. coronatae herb extract enhances the possibility of their use in pharmaceutical and cosmetic products with high levels of phytoecdysteroids. Moreover, this study has shown a positive effect of ecdysteroids-containing cream on changes in quality of life and a beneficial effect in reducing the symptoms of seborrheic dermatitis. It has been demonstrated that the application of the cream with phytoecdysteroids resulted in a statistically significant alleviation of symptoms (p &lt; 0.05), especially in terms of itching, pain or burning sensations in the affected areas in comparison to previous symptoms.<\/jats:p>","DOI":"10.3390\/ijerph17186453","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T11:24:24Z","timestamp":1599218664000},"page":"6453","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Separation and HPLC Characterization of Active Natural Steroids in a Standardized Extract from the Serratula coronata Herb with Antiseborrheic Dermatitis Activity"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3295-2546","authenticated-orcid":false,"given":"Marta","family":"Napiera\u0142a","sequence":"first","affiliation":[{"name":"Laboratory of Environmental Research, Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Street, 60-631 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1904-5503","authenticated-orcid":false,"given":"Joanna","family":"Nawrot","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Cosmetic Natural Products, Poznan University of Medical Sciences, 33 Mazowiecka Street, 60-623 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3106-064X","authenticated-orcid":false,"given":"Justyna","family":"Gornowicz-Porowska","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Cosmetic Natural Products, Poznan University of Medical Sciences, 33 Mazowiecka Street, 60-623 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2689-3009","authenticated-orcid":false,"given":"Ewa","family":"Florek","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Research, Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Street, 60-631 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arletta","family":"Moroch","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Cosmetic Natural Products, Poznan University of Medical Sciences, 33 Mazowiecka Street, 60-623 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zygmunt","family":"Adamski","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Poznan University of Medical Sciences, 49 Przybyszewskiego Street, 60-356 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kroma","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Cosmetic Natural Products, Poznan University of Medical Sciences, 33 Mazowiecka Street, 60-623 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0751-4867","authenticated-orcid":false,"given":"Izabela","family":"Miechowicz","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Statistics, Poznan University of Medical Sciences, 79 Dabrowskiego Street, 60-529 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Medicinal and Cosmetic Natural Products, Poznan University of Medical Sciences, 33 Mazowiecka Street, 60-623 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0031-9422(01)00078-4","article-title":"Phytoecdysteroids: Biological aspects","volume":"57","author":"Dinan","year":"2001","journal-title":"Phytochem"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.chroma.2005.05.004","article-title":"Analysis of ecdysteroids by micellar electrokinetic chromatography with on-line preconcentration","volume":"1081","author":"Santagati","year":"2005","journal-title":"J. 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