{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T13:47:47Z","timestamp":1762782467481,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System","award":["No. CARS-36"],"award-info":[{"award-number":["No. CARS-36"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Obesity has become a global health problem. Research suggests that leptin, a hormone that responds to fat deposition, may be involved in mammalian reproduction; however, its precise role in embryo implantation is poorly understood. Here, primary porcine endometrium epithelium cells (PEECs) were cultured in vitro and used to evaluate the regulatory role of different leptin levels on \u03b23-integrin, MMP9, HB-EGF, and IL-1\u03b2, which are, respectively, involved in four critical steps of embryo implantation. Results showed that only 0.01 nM leptin significantly improved \u03b23-integrin mRNA expression (p &lt; 0.05). MMP9 and HB-EGF mRNA expressions were upregulated by 0.10\u201310.00 nM leptin (p &lt; 0.05). The IL-1\u03b2 expression level was only increased by 10.00 nM leptin (p &lt; 0.05). \u03b23-integrin, MMP9, HB-EGF, and IL-1\u03b2 mRNA and protein have a similar fluctuant response to increased leptin. Leptin\u2019s influence on \u03b23-integrin, MMP9, HB-EGF, and IL-1\u03b2 disappeared when the JAK2, PI(3)K, or MAPK signaling pathways were blocked, respectively. In conclusion, leptin affected porcine implantation by regulating the expression of \u03b23-integrin, MMP9, HB-EGF, and IL-1\u03b2 in a dose-dependent manner. The signaling pathways of JAK2, PI(3)K, and MAPK may participate in this regulatory process. These findings will contribute to further understanding the mechanisms of reproductive disorders in obesity.<\/jats:p>","DOI":"10.3390\/ijerph17186508","type":"journal-article","created":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T09:18:16Z","timestamp":1599470296000},"page":"6508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Leptin Upregulates the Expression of \u03b23-Integrin, MMP9, HB-EGF, and IL-1\u03b2 in Primary Porcine Endometrium Epithelial Cells In Vitro"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8143-211X","authenticated-orcid":false,"given":"Hongfang","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"},{"name":"College of Animal Science and Technology, Shandong Agricultural University, Tai\u2019an 271018, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlian","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiguo","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1038\/nm0995-950","article-title":"Overexpression of the obese (ob) gene in adipose tissue of human obese subjects","volume":"1","author":"Arner","year":"1995","journal-title":"Nat. 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