{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:00:56Z","timestamp":1790913656210,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005888","name":"Uniwersytet Medyczny w Lodzi","doi-asserted-by":"publisher","award":["503\/1-168-01\/503-11-001"],"award-info":[{"award-number":["503\/1-168-01\/503-11-001"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The Fenton reaction (Fe2++H2O2\u2192Fe3++\u2022OH+OH-) results in strong oxidative damage to macromolecules when iron (Fe) or hydrogen peroxide (H2O2) are in excess. This study aims at comparing Fe2++H2O2-induced oxidative damage to membrane lipids (lipid peroxidation, LPO) and protective effects of 17\u03b2-estradiol (a potential antioxidant) in porcine ovary and thyroid homogenates. Iron, as one of the Fenton reaction substrates, was used in the highest achievable concentrations. Thyroid or ovary homogenates were incubated in the presence of: (1st) FeSO4+H2O2 with\/without 17\u03b2-estradiol (1 mM; 100, 10.0, 1.0 \u00b5M; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM); five experiments were performed with different FeSO4 concentrations (2400, 1200, 600, 300, 150 \u00b5M); (2nd) FeSO4 (2400, 1200, 600, 300, 150 \u00b5M)+H2O2 with\/without 17\u03b2-estradiol; three experiments were performed with three highest 17\u03b2-estradiol concentrations; (3rd) FeSO4 (2400, 1200, 1100, 1000, 900, 800, 700, 600, 300, 150, 75 \u00b5M)+H2O2 (5 mM). LPO level [MDA+4-HDA\/mg protein] was measured spectrophotometrically. The basal LPO level is lower in ovary than in thyroid homogenates. However, experimentally-induced LPO was higher in the former tissue, which was confirmed for the three highest Fe2+ concentrations (2400, 1200, 1100 \u00b5M). Exogenous 17\u03b2-estradiol (1 mM, 100, and 10 \u00b5M) reduced experimentally-induced LPO independently of iron concentration and that protective effect did not differ between tissues. The ovary, compared to the thyroid, reveals higher sensitivity to prooxidative effects of iron, however, it showed similar responsivity to protective 17\u03b2-estradiol activity. The therapeutic effect of 17\u03b2-estradiol against iron overload consequences should be considered with relation to both tissues.<\/jats:p>","DOI":"10.3390\/ijerph17186841","type":"journal-article","created":{"date-parts":[[2020,9,19]],"date-time":"2020-09-19T07:06:09Z","timestamp":1600499169000},"page":"6841","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17\u03b2-Estradiol in Porcine Ovary and Thyroid Homogenates"],"prefix":"10.3390","volume":"17","author":[{"given":"Aleksandra","family":"Rynkowska","sequence":"first","affiliation":[{"name":"Department of Oncological Endocrinology, Medical University of \u0141\u00f3d\u017a, 90-752 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7547-6228","authenticated-orcid":false,"given":"Jan","family":"St\u0119pniak","sequence":"additional","affiliation":[{"name":"Department of Oncological Endocrinology, Medical University of \u0141\u00f3d\u017a, 90-752 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3045-5669","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Karbownik-Lewi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Oncological Endocrinology, Medical University of \u0141\u00f3d\u017a, 90-752 \u0141\u00f3d\u017a, Poland"},{"name":"Polish Mother\u2019s Memorial Hospital\u2014Research Institute, 93-338 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/j.1753-4887.1994.tb01453.x","article-title":"Free radicals and antioxidants: A personal view","volume":"52","author":"Halliwell","year":"1994","journal-title":"Nutr. 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