{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T04:24:18Z","timestamp":1768278258235,"version":"3.49.0"},"reference-count":34,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The American cutaneous (CL) and visceral leishmaniasis (VL) are zooanthroponoses transmitted by sand flies. Brazil records thousands of human leishmaniasis cases annually. Dogs are reservoirs of Leishmania infantum, which causes VL, but their role in the transmission cycle of CL is debatable. Wild mammals are considered reservoirs of the aetiological agents of CL (Leishmania spp.). Objective: To describe the aetiology of leishmaniasis in dogs in an endemic area for CL and VL in the Amazon, Brazil. Methods: Clinical evaluation and blood collection of 40 dogs from the villages Ubim (20) and Socorro (20), city of Tom\u00e9-A\u00e7u, state of Par\u00e1, were carried out. The DNA extracted from the blood was used for PCR with Leishmania-specific primers targeting the hsp70-234 gene sequence. Products were sequenced (ABI3500XL), and the sequences were aligned, edited (BioEdit), and analyzed (Blastn). Results: Of the 34 amplified samples, 21 were sequenced, namely Leishmania infantum (12), L. guyanensis (5), L. braziliensis (3), and Leishmania sp. (01). Conclusion: Given the diversity of circulating pathogens, elucidation of the role of the dog in the Leishmania spp. cycle in Amazonian villages is imperative to the surveillance of CL in the region. We present the first report in Brazil, confirmed by sequencing, of canine infection by L. guyanensis, a species highly resistant to treatment in humans, with the drug of first choice (Glucantime\u00ae).<\/jats:p>","DOI":"10.3390\/ijerph17228488","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T21:48:52Z","timestamp":1605563332000},"page":"8488","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["First Report of Canine Infection by Leishmania (Viannia) guyanensis in the Brazilian Amazon"],"prefix":"10.3390","volume":"17","author":[{"given":"Francisco J. A.","family":"Santos","sequence":"first","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade,  Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana C. S.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade,  Ananindeua 67030-000, Par\u00e1, Brazil"},{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade do Estado do Par\u00e1,  Bel\u00e9m 66095-662, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8057-0836","authenticated-orcid":false,"given":"Wellington B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro Nacional de Primatas, Instituto Evandro Chagas, Minist\u00e9rio da Sa\u00fade, Secretaria de Vigil\u00e2ncia em Sa\u00fade,  Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana P.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade,  Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1857-9634","authenticated-orcid":false,"given":"Walter S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade,  Ananindeua 67030-000, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9075-9947","authenticated-orcid":false,"given":"D\u00e9lia C. F.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1,  Bel\u00e9m 66075-110, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourdes M.","family":"Garcez","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade,  Ananindeua 67030-000, Par\u00e1, Brazil"},{"name":"Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade do Estado do Par\u00e1,  Bel\u00e9m 66095-662, Par\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Alvar, J., V\u00e9lez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., and Boer, M.D. (2012). 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