{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:13:13Z","timestamp":1790838793111,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Research Council of norway","doi-asserted-by":"publisher","award":["281077"],"award-info":[{"award-number":["281077"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJERPH"],"abstract":"<jats:p>Dengue is the most rapidly spreading mosquito-borne viral disease in the world. The detection of clinical cases enables us to measure the incidence of dengue infection, whereas serological surveys give insights into the prevalence of infection. This study aimed to determine dengue seroprevalence and seroconversion rates in northeastern Thailand and southern Laos and to assess any association of mosquito control methods and socioeconomic factors with dengue virus (DENV) infection. Cross-sectional seroprevalence surveys were performed in May and November 2019 on the same individuals. Blood samples were collected from one adult and one child, when possible, in each of 720 randomly selected households from two urban and two rural sites in both northeastern Thailand and southern Laos. IgG antibodies against DENV were detected in serum using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Overall, 1071 individuals participated in the study. The seroprevalence rate was high (91.5%) across all 8 study sites. Only age and province were associated with seroprevalence rates. There were 33 seroconversions during the period from May to November, of which seven reported fever. More than half of the seroconversions occurred in the rural areas and in Laos. Dengue seroconversion was significantly associated with young age (&lt;15 years old), female gender, province, and duration of living in the current residence. No socioeconomic factors or mosquito control methods were found to be associated with seroprevalence or seroconversion. Notably, however, the province with most seroconversions had lower diurnal temperature ranges than elsewhere. In conclusion, our study has highlighted the homogeneity of dengue exposure across a wide range of settings and most notably those from rural and urban areas. Dengue can no longer be considered to be solely an urban disease nor necessarily one linked to poverty.<\/jats:p>","DOI":"10.3390\/ijerph17239134","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T12:24:39Z","timestamp":1607343879000},"page":"9134","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Dengue Seroprevalence and Seroconversion in Urban and Rural Populations in Northeastern Thailand and Southern Laos"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2664-3819","authenticated-orcid":false,"given":"Dyna","family":"Doum","sequence":"first","affiliation":[{"name":"Tropical Medicine Graduate Program, Academic Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans J.","family":"Overgaard","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 \u00c5s, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayfong","family":"Mayxay","sequence":"additional","affiliation":[{"name":"Institute of Research and Education Development (IRED), University of Health Sciences, Ministry of Health, P.O. Box 7444, Vientiane 43130, Laos"},{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Mahosot Hospital, Vientiane 43130, Laos"},{"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Old Road Campus, University of Oxford, Oxford OX3 7LG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sutas","family":"Suttiprapa","sequence":"additional","affiliation":[{"name":"Tropical Medicine Graduate Program, Academic Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasert","family":"Saichua","sequence":"additional","affiliation":[{"name":"Tropical Medicine Graduate Program, Academic Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tipaya","family":"Ekalaksananan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"HPV &amp; EBV and Carcinogenesis Research Group, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panwad","family":"Tongchai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"HPV &amp; EBV and Carcinogenesis Research Group, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8925-6544","authenticated-orcid":false,"given":"Md. Siddikur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Statistics, Begum Rokeya University, Rangpur 5400, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7413-1017","authenticated-orcid":false,"given":"Ubydul","family":"Haque","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology, University of North Texas Health Science Center, Fort Worth, TX 76177, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sysavanh","family":"Phommachanh","sequence":"additional","affiliation":[{"name":"Institute of Research and Education Development (IRED), University of Health Sciences, Ministry of Health, P.O. 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