{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:55:52Z","timestamp":1784681752276,"version":"3.55.0"},"reference-count":26,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T00:00:00Z","timestamp":1608854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (blaKPC) in Psittaciformes rescued from wildlife trafficking in Para\u00edba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby\u2013Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, blaKPC-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only blaKPC was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.<\/jats:p>","DOI":"10.3390\/ijerph18010095","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T09:30:19Z","timestamp":1608888619000},"page":"95","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Para\u00edba, Brazil"],"prefix":"10.3390","volume":"18","author":[{"given":"Gedean Galdino da Cruz","family":"Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias, Universidade Federal da Para\u00edba, Areia-PB 58397-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloiza Helena","family":"Campana","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Cl\u00ednica (LMC), Departamento de Ci\u00eancias Farmac\u00eauticas, Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal da Para\u00edba, Jo\u00e3o Pessoa-PB 58033-455, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7578-4087","authenticated-orcid":false,"given":"Priscylla Carvalho","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias, Universidade Federal da Para\u00edba, Areia-PB 58397-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00fabia Michelle Vieira da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias, Universidade Federal da Para\u00edba, Areia-PB 58397-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauro","family":"Santos Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Cl\u00ednica (LMC), Departamento de Ci\u00eancias Farmac\u00eauticas, Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal da Para\u00edba, Jo\u00e3o Pessoa-PB 58033-455, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9497-9819","authenticated-orcid":false,"given":"Elma Lima","family":"Leite","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias, Universidade Federal da Para\u00edba, Areia-PB 58397-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Em\u00edlia Naves","family":"Givisiez","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias, Universidade Federal da Para\u00edba, Areia-PB 58397-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wondwossen Abebe","family":"Gebreyes","sequence":"additional","affiliation":[{"name":"Global One Health Initiative (GOHi), Ohio State University, Columbus, OH 43210, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7761-0697","authenticated-orcid":false,"given":"Celso Jos\u00e9 Bruno de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ci\u00eancias Agr\u00e1rias, Universidade Federal da Para\u00edba, Areia-PB 58397-000, Brazil"},{"name":"Global One Health Initiative (GOHi), Ohio State University, Columbus, OH 43210, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1590\/1678-4162-10599","article-title":"Perfil de resist\u00eancia antimicrobiana de Klebsiella pneumoniae isoladas de animais dom\u00e9sticos e silvestres","volume":"71","author":"Sousa","year":"2019","journal-title":"Arq. 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