{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T17:18:42Z","timestamp":1770139122720,"version":"3.49.0"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2015\/17\/B\/NZ7\/02952"],"award-info":[{"award-number":["2015\/17\/B\/NZ7\/02952"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Although simultaneous supplementation of iron and folic acid is justified, the potential interactions between these micronutrients and other elements are poorly known. In this study, we aimed to investigate the effect of iron and folic acid supplementation on the levels of selected essential and toxic elements in the serum of micronutrient-deficient young women. A total of 40 women participated in this study and were divided into two groups: study group (n = 23) (with iron and folate deficiency) and control group (n = 17). The study group received iron and folic acid supplements for 3 months. Blood samples were collected at baseline and after the completion of the study period. Women completed a 3-day food intake record. We calculated the body mass index (BMI) of all the participants. Cellular morphology was analyzed in whole blood, and biochemical parameters were determined in serum. Elements were measured in serum by inductively coupled plasma mass spectrometry (ICP-MS). According to our results, in the case of the study group, the supplementation of iron and folic acid restored their levels; however, it caused a significant decrease in the level of zinc, calcium, and magnesium. In the case of the control group, at the end of the study period, there was a marked decrease in the level of iron. Interestingly, there was an increase in the level of arsenic and vanadium in both groups. In conclusion, simultaneous supplementation of iron and folic acid impairs the level of zinc, calcium, and magnesium in women of childbearing age.<\/jats:p>","DOI":"10.3390\/ijerph18031360","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T20:52:01Z","timestamp":1612299121000},"page":"1360","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Iron and Folic Acid Supplementation on the Level of Essential and Toxic Elements in Young Women"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0937-8427","authenticated-orcid":false,"given":"Joanna","family":"Suliburska","sequence":"first","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Poznan University of Life Science, ul. Wojska Polskiego 31, 60-624 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2045-0709","authenticated-orcid":false,"given":"Agata","family":"Chmurzynska","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Poznan University of Life Science, ul. Wojska Polskiego 31, 60-624 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4094-7103","authenticated-orcid":false,"given":"Rafal","family":"Kocylowski","sequence":"additional","affiliation":[{"name":"PreMediCare New Med Medical Center, ul. Dru\u017cbickiego 13, 61-693 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4173-5965","authenticated-orcid":false,"given":"Katarzyna","family":"Skrypnik","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Poznan University of Life Science, ul. Wojska Polskiego 31, 60-624 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Radziejewska","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition and Dietetics, Poznan University of Life Science, ul. Wojska Polskiego 31, 60-624 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danuta","family":"Baralkiewicz","sequence":"additional","affiliation":[{"name":"Department of Trace Element Analysis by Spectroscopy Method, Faculty of Chemistry, Adam Mickiewicz University in Poznan, ul. Umultowska 89b, 61-614 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2018). Weekly Iron and Folic Acid Supplementation as an Anaemia-Prevention Strategy in Women and Adolescent Girls Lessons Learnt from Implementation of Programmes among Non-Pregnant Women of Reproductive Age, WHO."},{"key":"ref_2","unstructured":"WHO (2012). Guideline: Daily Iron and Folic Acid Supplementation in Pregnant Women, World Health Organization."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3961\/jpmph.14.036","article-title":"Environmental source of arsenic exposure","volume":"47","author":"Chung","year":"2014","journal-title":"J. Prev. Med. Public Health"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Radziejewska, A., Suliburska, J., Ko\u0142odziejski, P., and Chmurzynska, A. (2020). Role of Slc19a1 and Tfr2 in liver transport of iron and folate: A rat model of folate\/iron deficiency followed by supplementation. J. Trace Elem. Med. Biol., 62.","DOI":"10.1016\/j.jtemb.2020.126568"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1017\/S0007114519002721","article-title":"Simultaneous supplementation with iron and folic acid can affect Slc11a2 and Slc46a1 transcription and metabolite concentrations in rats","volume":"123","author":"Radziejewska","year":"2020","journal-title":"Br. J. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1007\/s12011-019-01888-z","article-title":"Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients","volume":"195","author":"Suliburska","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.3945\/ajcn.2010.28674F","article-title":"Iron bioavailability and dietary reference values","volume":"91","author":"Hurrell","year":"2010","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Skrypnik, K., and Suliburska, J. (2018). Association between the gut microbiota and mineral metabolism. J. Sci. Food Agric., 98.","DOI":"10.1002\/jsfa.8724"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1021\/jf9807622","article-title":"The importance of the proportion of heme\/nonheme iron in the diet to minimize the interference with calcium, phosphorus, and magnesium metabolism on recovery from nutritional ferropenic anemia","volume":"47","author":"Lisbona","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jtemb.2017.02.005","article-title":"Interactions of iron with manganese, zinc, chromium, and selenium as related to prophylaxis and treatment of iron deficiency","volume":"41","author":"Aaseth","year":"2017","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2693","DOI":"10.1007\/s00394-017-1535-7","article-title":"Calcium and zinc decrease intracellular iron by decreasing transport during iron repletion in an in vitro model","volume":"57","author":"Lautaro","year":"2018","journal-title":"Eur. J. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1097\/XEB.0000000000000212","article-title":"Intermittent iron supplementation for reducing anaemia and its associated impairments in adolescent and adult menstruating women","volume":"18","author":"McLoughlin","year":"2020","journal-title":"Int. J. Evid. Based. Healthc."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tsang, B., Sandalinas, F., and De-Regil, L.M. (2015). Folate supplementation in women of reproductive age. Cochrane Database Syst. Rev., 2015.","DOI":"10.1002\/14651858.CD011766"},{"key":"ref_14","unstructured":"World Health Organization (2016). Women\u2019s Health and Well-Being in Europe: Beyond the Mortality Advantage, WHO."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5103","DOI":"10.3390\/nu6115103","article-title":"Micronutrient status in female university students: Iron, zinc, copper, selenium, vitamin B12 and folate","volume":"6","author":"Petocz","year":"2014","journal-title":"Nutrients"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"438","DOI":"10.3109\/03014460.2015.1071424","article-title":"Age-related differences in hair trace elements: A cross-sectional study in Orenburg, Russia","volume":"43","author":"Skalnaya","year":"2016","journal-title":"Ann. Hum. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Suliburska, J. (2011). A comparison of levels of select minerals in scalp hair samples with estimated dietary intakes of these minerals in women of reproductive age. Biol. Trace Elem. Res., 144.","DOI":"10.1007\/s12011-011-9034-9"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5707","DOI":"10.3748\/wjg.v13.i43.5707","article-title":"Concurrent repletion of iron and zinc reduces intestinal oxidative damage in iron- and zinc-deficient rats","volume":"13","author":"Bodiga","year":"2007","journal-title":"World J. Gastroenterol."},{"key":"ref_19","unstructured":"Stargrove, M., Treasure, J., and McKee, D. (2008). Herb, Nutrient, and Drug Interactions, Mosby Elsevier."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s12291-017-0634-9","article-title":"Micronutrients Drift During Daily and Weekly Iron Supplementation in Non-anaemic and Anaemic Pregnancy","volume":"32","author":"Shankar","year":"2017","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_21","first-page":"24","article-title":"Factors Affecting Iron Absorption and Mitigation Mechanisms: A review","volume":"4","author":"Dasa","year":"2018","journal-title":"Int. J. Agric. Sci. Food Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1024\/0300-9831\/a000036","article-title":"Calcium and iron absorption\u2014Mechanisms and public health relevance","volume":"80","year":"2010","journal-title":"Int. J. Vitam. Nutr. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Suliburska, J. (2014). The impact of iron content in a diet high in fat, fructose, and salt on metabolic state and mineral status of rats. J. Physiol. Biochem., 70.","DOI":"10.1007\/s13105-013-0276-1"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s12576-018-0600-1","article-title":"Intestinal calcium transport and its regulation in thalassemia: Interaction between calcium and iron metabolism","volume":"68","author":"Lertsuwan","year":"2018","journal-title":"J. Physiol. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s12291-017-0653-6","article-title":"Study on Impact of Iron and Folic Acid on the Plasma Trace Minerals in Pregnant Anemic Women","volume":"33","author":"Tiwari","year":"2018","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.clnu.2010.08.001","article-title":"Oral iron supplementation leads to oxidative imbalance in anemic women: A prospective study","volume":"30","author":"Mahdi","year":"2011","journal-title":"Clin. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1093\/jn\/nxz046","article-title":"Antenatal multiple micronutrient supplementation compared to iron-folic acid affects micronutrient status but does not eliminate deficiencies in a randomized controlled trial among pregnant women of rural Bangladesh","volume":"149","author":"Schulze","year":"2019","journal-title":"J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Kocy\u0142owski, R., Lewicka, I., Grzesiak, M., Gaj, Z., Soba\u0144ska, A., Poznaniak, J., von Kaisenberg, C., and Suliburska, J. (2018). Assessment of dietary intake and mineral status in pregnant women. Arch. Gynecol. Obs., 297.","DOI":"10.1007\/s00404-018-4744-2"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3177\/jnsv.64.1","article-title":"Absorption mechanisms of iron, copper, and zinc: An overview","volume":"64","author":"Nishito","year":"2018","journal-title":"J. Nutr. Sci. Vitam. (Tokyo)"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.3390\/ijerph8061991","article-title":"Urinary arsenic metabolites of subjects exposed to elevated arsenic present in coal in Shaanxi Province, China","volume":"8","author":"Gao","year":"2011","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.jsm.2017.12.007","article-title":"Human health and environmental impacts of coal combustion and post-combustion wastes","volume":"17","author":"Munawer","year":"2018","journal-title":"J. Sustain. Min."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zwolak, I. (2020). Protective Effects of Dietary Antioxidants against Vanadium-Induced Toxicity: A Review. Oxid. Med. Cell. Longev., 2020.","DOI":"10.1155\/2020\/1490316"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1051\/e3sconf\/20130103004","article-title":"Anthropogenic Vanadium emissions to air and ambient air concentrations in North-West Europe","volume":"1","author":"Visschedijk","year":"2013","journal-title":"E3S Web Conf."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/j.chemosphere.2018.11.115","article-title":"Trace element profiles in pregnant women\u2019s sera and umbilical cord sera and influencing factors: Repeated measurements","volume":"218","author":"Liang","year":"2019","journal-title":"Chemosphere"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"109178","DOI":"10.1016\/j.envres.2020.109178","article-title":"Predictors of urinary and blood metal(loid) concentrations among pregnant women in Northern Puerto Rico","volume":"183","author":"Ashrap","year":"2020","journal-title":"Environ. Res."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/18\/3\/1360\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:19:09Z","timestamp":1760159949000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/18\/3\/1360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,2]]},"references-count":35,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["ijerph18031360"],"URL":"https:\/\/doi.org\/10.3390\/ijerph18031360","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,2]]}}}