{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T01:31:21Z","timestamp":1782437481070,"version":"3.54.5"},"reference-count":27,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1A2C1102228"],"award-info":[{"award-number":["NRF-2020R1A2C1102228"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Busan 21 Plus project","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Human papillomavirus (HPV) infection in males is associated with various cancers, including cervical cancer in women and penile and bladder cancers in men. However, there is limited research on the prevalence and prevention of male HPV infection. Moreover, a rapid test that can prevent the increase in HPV infection is needed. In this study, the prevalence of sexually transmitted pathogen (STP) and HPV infection was analyzed using real-time polymerase chain reaction assay in random urine samples collected from asymptomatic male sexual partners of women with sexually transmitted diseases. Among 130 men, 65 (50.0%) had STP and 12 (9.23%) had HPV infection. There was no association between STP and HPV infection. Among 12 cases of HPV infection, three were HPV-16 single infections, six were multiple infections, including HPV-16, and three of other high-risk HPV infections. Our results suggest the need for STP testing, including HPV testing, in sexual partners of high-risk women with sexually transmitted diseases, even in men without clinical symptoms (asymptomatic). Further research should be conducted by diversifying urine samples. We report the most convenient method for HPV detection, and it is expected to be widely applied to prevent sexually transmitted diseases in men and women.<\/jats:p>","DOI":"10.3390\/ijerph182111706","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T08:05:16Z","timestamp":1636358716000},"page":"11706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Human Papillomavirus Prevalence in Urine Samples of Asymptomatic Male Sexual Partners of Women with Sexually Transmitted Diseases"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1773-023X","authenticated-orcid":false,"given":"Hyunwoo","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, The Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5147-1783","authenticated-orcid":false,"given":"Dong Hyeok","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, The Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9543-4159","authenticated-orcid":false,"given":"Kyung Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea"},{"name":"Clinical Trial Specialist Program for In Vitro Diagnostics, Brain Busan 21 Plus Program, The Graduate School, Catholic University of Pusan, Busan 46252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1016\/j.vaccine.2006.05.111","article-title":"The Burden of HPV Related Cancers","volume":"24","author":"Parkin","year":"2006","journal-title":"Vaccine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e609","DOI":"10.1016\/S2214-109X(16)30143-7","article-title":"Global Burden of Cancers Attributable to Infections in 2012: A Synthetic Analysis","volume":"4","author":"Plummer","year":"2016","journal-title":"Lancet Glob. Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1002\/jmv.24422","article-title":"Human Papillomavirus Infection in Male Patients with STI-Related Symptoms in Hanoi, Vietnam","volume":"88","author":"Le","year":"2016","journal-title":"J. Med. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.jadohealth.2011.03.010","article-title":"Age-Specific Prevalence of Human Papillomavirus Infection in Males: A Global Review","volume":"48","author":"Smith","year":"2011","journal-title":"J. Adolesc. Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.jcv.2014.04.003","article-title":"Prevalence of HPV Infection Among Clinically Healthy Italian Males and Genotype Concordance Between Stable Sexual Partners","volume":"60","author":"Lorenzon","year":"2014","journal-title":"J. Clin. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1086\/368220","article-title":"Human Papillomavirus Infection in Men Attending a Sexually Transmitted Disease Clinic","volume":"187","author":"Baldwin","year":"2003","journal-title":"J. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1086\/507432","article-title":"Prevalence of HPV Infection among Men: A Systematic Review of the Literature","volume":"194","author":"Dunne","year":"2006","journal-title":"J. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"15637","DOI":"10.1038\/srep15637","article-title":"Concurrence of Oral and Genital Human Papillomavirus Infection in Healthy Men: A Population-Based Cross-Sectional Study in Rural China","volume":"5","author":"Liu","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1093\/infdis\/jix116","article-title":"Concordance of Penile and Oral Human Papillomavirus Infections among Men in the United States","volume":"215","author":"Patel","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1086\/521629","article-title":"The Optimal Anatomic Sites for Sampling Heterosexual Men for Human Papillomavirus (HPV) Detection: The HPV Detection in Men Study","volume":"196","author":"Giuliano","year":"2007","journal-title":"J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1442-2042.2010.02521.x","article-title":"Prevalence of Human Papillomavirus Infection in the Urinary Tract of Men with Urethritis","volume":"17","author":"Shigehara","year":"2010","journal-title":"Int. J. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1097\/00007435-199703000-00008","article-title":"Human Papillomavirus DNA in Urine Specimens of Men with Condyloma Acuminatum","volume":"24","author":"Iwasawa","year":"1997","journal-title":"Sex. Transm. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1086\/381395","article-title":"Evaluation of Genital Sites and Sampling Techniques for Detection of Human Papillomavirus DNA in Men","volume":"189","author":"Weaver","year":"2004","journal-title":"J. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1128\/jcm.27.8.1711-1714.1989","article-title":"Human Papillomavirus Detection in Urine Samples from Male Patients by the Polymerase Chain Reaction","volume":"27","author":"Melchers","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1056\/NEJM198710083171502","article-title":"High Prevalence of Papillomavirus-Associated Penile Intraepithelial Neoplasia in Sexual Partners of Women with Cervical Intraepithelial Neoplasia","volume":"317","author":"Barrasso","year":"1987","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/S0140-6736(85)91724-6","article-title":"Increased Risk of Cervical Neoplasia in Consorts of Men with Penile Condylomata Acuminata","volume":"1","author":"Campion","year":"1985","journal-title":"Lancet"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jviromet.2004.11.011","article-title":"Human Papillomavirus DNA Detection and Typing in Male Urine Samples from a High-Risk Population from Argentina","volume":"124","author":"Golijow","year":"2005","journal-title":"J. Virol. Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/pros.10157","article-title":"Detection of Human Polyomaviruses and Papillomaviruses in Prostatic Tissue Reveals the Prostate as a Habitat for Multiple Viral Infections","volume":"53","author":"Zambrano","year":"2002","journal-title":"Prostate"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1097\/00007435-200303000-00014","article-title":"Poor Sensitivity of Polymerase Chain Reaction Assays of Genital Skin Swabs and Urine to Detect HPV 6 and 11 DNA in Men","volume":"30","author":"Fife","year":"2003","journal-title":"Sex. Transm. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.jiac.2014.08.033","article-title":"Human Papillomavirus Infection and Pathogenesis in Urothelial Cells: A Mini-Review","volume":"20","author":"Shigehara","year":"2014","journal-title":"J. Infect. Chemother."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Daponte, A., Michail, G., Daponte, A.I., Daponte, N., and Valasoulis, G. (2021). Urine HPV in the Context of Genital and Cervical Cancer Screening-An Update of Current Literature. Cancers, 13.","DOI":"10.3390\/cancers13071640"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1111\/j.1469-0691.2008.01974.x","article-title":"Use of real-time PCR to detect human papillomavirus-16 viral loads in vaginal and urine self-sampled specimens","volume":"14","author":"Daponte","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1007\/s10096-017-3179-1","article-title":"Human papillomavirus genotype and viral load agreement between paired first-void urine and clinician-collected cervical samples","volume":"37","author":"Tjalma","year":"2018","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jviromet.2018.11.008","article-title":"Human papillomavirus detection in urine: Effect of a first-void urine collection device and timing of collection","volume":"264","author":"Pattyn","year":"2019","journal-title":"J. Virol. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/S1386-6532(03)00157-4","article-title":"Optimization of PCR Based Detection of Human Papillomavirus DNA from Urine Specimens","volume":"29","author":"Brinkman","year":"2004","journal-title":"J. Clin. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1128\/JCM.05219-11","article-title":"Liquid-Based Urine Cytology as a Tool for Detection of Human Papillomavirus, Mycoplasma spp., and Ureaplasma spp. in Men","volume":"50","author":"Kawaguchi","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1097\/OLQ.0b013e318289c186","article-title":"Association of Chlamydia trachomatis Infection and Herpes Simplex Virus type 2 Serostatus with Genital Human Papillomavirus Infection in Men: The HPV in Men Study","volume":"40","author":"Alberts","year":"2013","journal-title":"Sex. Transm. 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