{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:08:56Z","timestamp":1769072936932,"version":"3.49.0"},"reference-count":51,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T00:00:00Z","timestamp":1646179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Anal abscesses and fistulas are potential complications of Crohn\u2019s disease (CD). Chronic immunosuppression, loose stools, and poor wound healing in this population present challenges to the management of perianal diseases. The purpose of the study was to determine the predominant bacterial species found in specimens from perianal lesions causing anal abscess and\/or fistula drainage in hospitalized patients, and to compare the number and type of microorganisms isolated from samples taken from patients undergoing biological therapy or traditionally treated. The outcomes of studies of patients treated for anal abscesses or fistulas from 2017 to 2019 were evaluated. Data obtained from medical records included culture and antibiotic sensitivity results of swabs from perianal lesions of isolated microorganisms. A total of 373 swabs were collected from perianal lesions during the analysis period, including 51 (49 positive samples) from patients with CD. The predominant pathogen was Escherichia coli (55%, p &lt; 0.001), the second most common microorganism was Staphylococcus aureus (14.3%, p &lt; 0.001). In vitro susceptibility testing showed E. coli, ESBL (strain with Extended Spectrum Beta-Lactamase) in five cases, S. aureus, MRSA (methicillin-resistant S. aureus -resistant to all beta-lactam antibiotics: penicillins with inhibitors, cephalosporins, monobactams, carbapenems, except for ceftaroline) in one sample. Biologic therapy does not affect the type of microorganisms isolated from perianal abscesses in patients with CD.<\/jats:p>","DOI":"10.3390\/ijerph19052892","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T08:37:16Z","timestamp":1646210236000},"page":"2892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Does Anti-TNF-\u03b1 Therapy Affect the Bacteriological Profile of Specimens Collected from Perianal Lesions? A Retrospective Analysis in Patients with Crohn\u2019s Disease"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1390-9278","authenticated-orcid":false,"given":"Jolanta","family":"Gruszecka","sequence":"first","affiliation":[{"name":"Institute of Health Sciences, Medical College of Rzeszow University, 35-310 Rzeszow, Poland"},{"name":"Department of Clinical Microbiology, Clinical Hospital No. 2 im. Sw. Jadwigi Kr\u00f3lowej, 35-301 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5954-151X","authenticated-orcid":false,"given":"Rafa\u0142","family":"Filip","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical College of Rzeszow University, 35-959 Rzeszow, Poland"},{"name":"Department of Gastroenterology with IBD, Unit of Clinical Hospital 2 in Rzeszow, Lwowska 60, 35-301 Rzeszow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,2]]},"reference":[{"key":"ref_1","first-page":"401","article-title":"Diagnosis and Treatment of Perianal Crohn Disease: NASPGHAN Clinical Report and Consensus Statement","volume":"57","author":"Pasternak","year":"2013","journal-title":"JPGN"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1093\/ecco-jcc\/jjv228","article-title":"Results of the Fifth Scientific Workshop of the ECCO (II): Pathophysiology of Perianal Fistulizing Disease","volume":"10","author":"Siegmund","year":"2016","journal-title":"J. Crohns Colitis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/apt.12365","article-title":"Colonisation by Faecalibacterium prausnitzii and maintenance of clinical remission in patients with ulcerative colitis","volume":"38","author":"Varela","year":"2013","journal-title":"Aliment Pharm."},{"key":"ref_4","first-page":"1219","article-title":"Recent advances in characterizing the gastrointestinal microbiome in Crohn\u2019s disease: A systematic review","volume":"21","author":"Wright","year":"2015","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1136\/gutjnl-2016-313235","article-title":"A microbial signature for Crohn\u2019s disease","volume":"66","author":"Pascal","year":"2017","journal-title":"Gut"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/ecco-jcc\/jjw168","article-title":"3rd European Evidence-based Consensus on the Diagnosis and Management of Crohn\u2019s Disease 2016: Part 1: Diagnosis and Medical Management","volume":"11","author":"Dignass","year":"2017","journal-title":"J. Crohn\u2019s Colitis"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/ecco-jcc\/jjw169","article-title":"3rd European Evidence-based Consensus on the Diagnosis and Management of Crohn\u2019s Disease 2016: Part 2: Surgical Management and Special Situations","volume":"11","author":"Gionchetti","year":"2017","journal-title":"J. Crohn\u2019s Colitis"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"A221","DOI":"10.1136\/gut.2011.239301.466","article-title":"The rectal mucosa in patients with crohn\u2019s anal fistulae harbours lower numbers of bifidobacteria, and the fistula tracts are devoid of a microbial ecosystem","volume":"60","author":"Tozer","year":"2011","journal-title":"Gut"},{"key":"ref_9","first-page":"27","article-title":"Bacteriology of anal fistulae","volume":"79","author":"Hay","year":"1992","journal-title":"Br. J. Surg."},{"key":"ref_10","first-page":"533","article-title":"Bacteriology of anal fistulae","volume":"94","author":"Chimeno","year":"2002","journal-title":"Rev. Esp. Enferm. Dig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1097\/MCG.0000000000000024","article-title":"Ciprofloxacin usage and bacterial resistance patterns in Crohn\u2019s disease patients with abscesses","volume":"48","author":"Park","year":"2014","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1007\/s10096-013-1912-y","article-title":"Identification of Enterobacteriaceae by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry using the VITEK MS system","volume":"32","author":"Richter","year":"2013","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1099\/jmm.0.080317-0","article-title":"Performance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China","volume":"64","author":"Luo","year":"2015","journal-title":"J Med. Microbiol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.bpg.2016.09.008","article-title":"Infectious complications in gastrointestinal endoscopy and their prevention","volume":"30","author":"Kovaleva","year":"2016","journal-title":"Best Pr. Res. Clin. Gastroenterol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1128\/JCM.02071-09","article-title":"High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry in conventional medical microbiology laboratories","volume":"48","author":"Veen","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1099\/jmm.0.053256-0","article-title":"Comparison of two matrix-assisted laser desorption\/ionization\u2014Time of flight (MALDI-TOF) mass spectrometry methods and API 20AN for identification of clinically relevant anaerobic bacteria","volume":"62","author":"Jamal","year":"2013","journal-title":"J. Med. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1097\/PAT.0b013e328358343c","article-title":"Comparison of Vitek MS (MALDI-TOF) to standard routine Identification methods: An advance but no panacea","volume":"44","author":"Harris","year":"2012","journal-title":"Pathology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3073","DOI":"10.1007\/s10096-012-1667-x","article-title":"Evaluation of species-specific PCR, Bruker MS, VITEK MS and the VITEK 2 system for the identification of clinical Enterococcus isolates","volume":"31","author":"Fang","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3522","DOI":"10.1128\/JCM.00403-07","article-title":"Multicenter Comparison of the VITEK 2 Antifungal Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Amphotericin B, Flucytosine, and Voriconazole against Candida spp.","volume":"45","author":"Pfaller","year":"2007","journal-title":"J. Clin. Mikrobiol."},{"key":"ref_20","first-page":"225","article-title":"The evaluation of bacteriology in perianal abscesses of 81 adult patients","volume":"14","author":"Ulug","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_21","unstructured":"Cohen, J., Powderly, W.G., and Opal, S.M. (2017). Section 8 Clinical Microbiology: Bacteria, 184 Anaerobic Bacteria. Infectious Diseases, Elsevier. [4th ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jcol.2019.11.489","article-title":"Anal abscess microbiology as an anal fistula predictor","volume":"40","author":"Enez","year":"2020","journal-title":"J. Coloproctol."},{"key":"ref_23","first-page":"356","article-title":"Routine operative swab cultures and post-operative antibiotic use for uncomplicated perianal abscesses are unnecessary","volume":"87","author":"Ngu","year":"2014","journal-title":"ANZ J. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1093\/ecco-jcc\/jjy017","article-title":"Microbial spectrum of intra-abdominal abscesses in perforating Crohn\u2019s disease: Results from a prospective German registry","volume":"12","author":"Reuken","year":"2018","journal-title":"J. Crohns Colitis"},{"key":"ref_25","first-page":"745","article-title":"Genus XXIX. Proteus","volume":"Volume 2","author":"Brenner","year":"2005","journal-title":"Bergey\u2019s Manual of Systematic Bacteriology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1136\/gutjnl-2015-309184","article-title":"GETAID Structural robustness of the gut mucosal microbiota is associated with Crohn\u2019s disease remission after surgery","volume":"65","author":"Mondot","year":"2016","journal-title":"Gut"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1093\/ecco-jcc\/jjw136","article-title":"Microbial factors associated with postoperative Crohn\u2019s disease recurrence","volume":"11","author":"Wright","year":"2017","journal-title":"J. Crohns Colitis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e00085-17","DOI":"10.1128\/CMR.00085-17","article-title":"Proteus spp. as putative gastrointestinal pathogens","volume":"31","author":"Hamilton","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1111\/j.1440-1746.2007.04964.x","article-title":"Education and imaging. Gastrointestinal: Abdominal abscess associated with a ventriculoperitoneal shunt","volume":"22","author":"Liu","year":"2007","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1111\/j.1532-5415.2006.00964.x","article-title":"Pathogenic colonization of the stomach in enterally fed elderly patients: Comparing percutaneous endoscopic gastrostomy with nasogastric tube","volume":"54","author":"Segal","year":"2006","journal-title":"J. Am. Geriatr. Soc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jhin.2005.11.005","article-title":"Gastric microbiota in elderly patients fed via nasogastric tubes for prolonged periods","volume":"63","author":"Segal","year":"2006","journal-title":"J. Hosp. Infect."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"S-389","DOI":"10.1016\/S0016-5085(14)61396-2","article-title":"Su1148. Bacterial biofilms produced in stents retrieved from patients with biliary and pancreatic diseases","volume":"146","author":"Vaishnavi","year":"2014","journal-title":"Gastroenterology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1007\/s00405-010-1253-8","article-title":"Microbial colonization of tracheoesophageal voice prostheses (Provox2) following total laryngectomy","volume":"267","author":"Ticac","year":"2010","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1590\/S0004-28032006000400002","article-title":"Microbiologic profile of flexible endoscope disinfection in two Brazilian hospitals","volume":"43","author":"Machado","year":"2006","journal-title":"Arq. Gastroenterol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kramer, A., Schwebke, I., and Kampf, G. (2006). How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect. Dis., 6.","DOI":"10.1186\/1471-2334-6-130"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1093\/infdis\/139.6.621","article-title":"A nosocomial outbreak of infections due to multiply resistant Proteus mirabilis: Role of intestinal colonization as a major reservoir","volume":"139","author":"Chow","year":"1979","journal-title":"J. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1007\/s10620-005-2769-4","article-title":"Perianal Fistulas in Crohn\u2019s Disease Are Predominantly Colonized by Skin Flora: Implications for Antibiotic Treatment?","volume":"50","author":"West","year":"2005","journal-title":"Dig. Dis. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Chen, C.-Y., Cheng, A., Huang, S.-Y., Sheng, W.-H., Liu, J.-H., Ko, B.-S., Yao, M., Chou, W.-C., Lin, H.-C., and Chen, Y.-C. (2013). Clinical and Microbiological Characteristics of Perianal Infections in Adult Patients with Acute Leukemia. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0060624"},{"key":"ref_39","unstructured":"Gordon, P.H., and Nivatours, S. (1992). Anorectal abscess and fi stula-in-ano. Principles and Practice of Surgery of the Colon, Rectum and Anus, Quality Medical Publishing."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1002\/bjs.1800810314","article-title":"Surgical assesment of acute anorectal sepsis is a better predictor of fistula than microbiological analysis","volume":"81","author":"Lunniss","year":"1994","journal-title":"Br. J. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/0022-3468(94)90587-8","article-title":"Fistula-in-ano in infancy and childhood","volume":"29","author":"Laing","year":"1994","journal-title":"J. Pediatr. Surg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1128\/jcm.35.11.2974-2976.1997","article-title":"The aerobic and anaerobic bacteriology of perirectal abscess","volume":"35","author":"Brook","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00384-006-0121-x","article-title":"Microbiological analysis and endoanal ultrasonography for diagnosis of anal fi stula in acute anorectal sepsis","volume":"22","author":"Toyonaga","year":"2007","journal-title":"Int. J. Colorectal Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/bjs.1800730515","article-title":"Incidence of fi stula-in-ano complicating anorectal sepsis: A prospective study","volume":"73","author":"Henrichsen","year":"1986","journal-title":"Br. J. Surg."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"175","DOI":"10.5455\/2349-2902.isj20150510","article-title":"The value of microbiological analysis of pus swabs in perianal abscess. Have they stood the test of time and antibiotic usage?","volume":"2","author":"Narayanan","year":"2015","journal-title":"Int. Surg. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1007\/s10151-013-1111-y","article-title":"Risk factors for anal fistula: A case\u2013control study","volume":"18","author":"Wang","year":"2014","journal-title":"Tech. Coloproctol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jss.2019.04.032","article-title":"A Distinct Gut Microbiota Exists Within Crohn\u2019s DiseaseeRelated Perianal Fistulae","volume":"242","author":"Haac","year":"2019","journal-title":"J. Surg. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"114","DOI":"10.14740\/gr1279","article-title":"Auditing the Routine Microbiological Examination of Pus Swabs from Uncomplicated Perianal Abscesses: Clinical Necessity or Old Habit?","volume":"13","author":"Lalou","year":"2020","journal-title":"Gastroenterol. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1007\/s00268-009-0190-4","article-title":"Pus Swabs in Incision and Drainage of Perianal Abscesses: What Is the Point?","volume":"33","author":"Leung","year":"2009","journal-title":"World J. Surg."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1093\/clinids\/10.1.42","article-title":"Anorectal infections in patients with malignant diseases","volume":"10","author":"Glenn","year":"1988","journal-title":"Rev. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2758","DOI":"10.3748\/wjg.v26.i21.2758","article-title":"Vaccine therapy for dysbiosis-related diseases","volume":"26","author":"Fujimoto","year":"2020","journal-title":"World J. Gastroenterol."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/5\/2892\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:30:31Z","timestamp":1760135431000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/5\/2892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,2]]},"references-count":51,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["ijerph19052892"],"URL":"https:\/\/doi.org\/10.3390\/ijerph19052892","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,2]]}}}