{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:27:08Z","timestamp":1772252828898,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006143","name":"Department of Science and Technology","doi-asserted-by":"publisher","award":["No grant number"],"award-info":[{"award-number":["No grant number"]}],"id":[{"id":"10.13039\/501100006143","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Our study assessed the impact of using ovitraps with pyriproxyfen on mosquito populations and the feasibility of using human saliva samples to test for seroconversion to dengue virus (DENV). We used a quasi-experimental research design by forming the intervention (n = 220) and the control (n = 223) groups in neighboring Taguig City, Philippines, over 4 months. Socio-demographic data, entomological indices, and IgG antibodies against DENV were measured. Associations between the implementation of ovitraps dosed with pyriproxyfen and mosquito densities (percentage positive ovitraps and container indices) and DENV seroconversion were calculated post-intervention in Months 2, 3, and 4. Among the participants recruited at baseline, 17 and 13 were seropositive for dengue (DENV) in the intervention and control groups, respectively. Both entomological indices were lower in the treated area than the control site at post-intervention Months 2, 3, and 4, but not earlier. Dengue seroconversions rates decreased in the treated population, but not significantly so. In conclusion, the use of PPF-treated ovitraps may have impacted the mosquito population, but not seroconversion rates. Compliance in providing saliva samples and the ability to detect IgG antibodies within these samples was encouraging and suggests that further studies on larger populations for longer durations are warranted.<\/jats:p>","DOI":"10.3390\/ijerph19053026","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:38:16Z","timestamp":1646599096000},"page":"3026","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Assessing Entomological and Epidemiological Efficacy of Pyriproxyfen-Treated Ovitraps in the Reduction of Aedes Species: A Quasi-Experiment on Dengue Infection Using Saliva Samples"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7469-7670","authenticated-orcid":false,"given":"Antonio D.","family":"Ligsay","sequence":"first","affiliation":[{"name":"The Graduate School & College of Science, University of Santo Tomas Espa\u00f1a Boulevard, Manila 1008, Philippines"},{"name":"Clinical Research Section, St. Luke\u2019s College of Medicine\u2014William H. Quasha Memorial, 279 E. Rodriguez Sr. Ave, Quezon City 1112, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristan Jela M.","family":"Tambio","sequence":"additional","affiliation":[{"name":"Clinical Research Section, St. Luke\u2019s College of Medicine\u2014William H. Quasha Memorial, 279 E. Rodriguez Sr. Ave, Quezon City 1112, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle Joyce M.","family":"Aytona","sequence":"additional","affiliation":[{"name":"Clinical Research Section, St. Luke\u2019s College of Medicine\u2014William H. Quasha Memorial, 279 E. Rodriguez Sr. Ave, Quezon City 1112, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4524-0385","authenticated-orcid":false,"given":"Grecebio Jonathan D.","family":"Alejandro","sequence":"additional","affiliation":[{"name":"The Graduate School & College of Science, University of Santo Tomas Espa\u00f1a Boulevard, Manila 1008, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-6336","authenticated-orcid":false,"given":"Zypher Jude G.","family":"Regencia","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology, College of Medicine, University of the Philippines Manila, Pedro Gil Street, Ermita, Manila 1000, Philippines"},{"name":"Institute of Clinical Epidemiology, National Institutes of Health, University of the Philippines Manila, 623 Pedro Gil Street, Ermita, Manila 1000, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3888-8880","authenticated-orcid":false,"given":"Emmanuel S.","family":"Baja","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology, College of Medicine, University of the Philippines Manila, Pedro Gil Street, Ermita, Manila 1000, Philippines"},{"name":"Institute of Clinical Epidemiology, National Institutes of Health, University of the Philippines Manila, 623 Pedro Gil Street, Ermita, Manila 1000, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0665-5089","authenticated-orcid":false,"given":"Richard Edward L.","family":"Paul","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Functional Genetics of Infectious Diseases Unit, UMR 2000 (CNRS), 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/S1473-3099(16)00026-8","article-title":"The global burden of dengue: An analysis from the Global Burden of Disease Study 2013","volume":"16","author":"Stanaway","year":"2016","journal-title":"Lancet Infect. 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