{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T11:45:24Z","timestamp":1771674324636,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T00:00:00Z","timestamp":1646697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009687","name":"Wroclaw Medical University","doi-asserted-by":"publisher","award":["statutory grant SUBZ.C290.22.090"],"award-info":[{"award-number":["statutory grant SUBZ.C290.22.090"]}],"id":[{"id":"10.13039\/501100009687","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Background: Healthcare workers (HCWs) are more exposed to influenza infection, and the influenza vaccination is recommended each year, to reduce the risk of influenza infection and prevent influenza transmission. This study is a cross-sectional study and the objectives were to determine the rate of influenza virus infection among HCWs in the 2019\u20132020 influenza season. Methods: Between January and March 2020, a survey was carried out in 2 hospitals and 15 primary health-care settings (PHCS) in Wroclaw (Poland). The novel point-of-care testing Flu SensDx device was used, which detects the M1 protein of the influenza virus using electrochemical impedance spectroscopy from biological material (throat\/nasal swabs). Results: A total of 150 samples were collected. The majority of participating HCWs by profession were 83 physicians (55.3%) and half (51.3%) of the participating HCWs worked in PHCS. Influenza vaccination coverage was 61.3% in 2019\u20132020 and 46.0% in the 2018\u20132019 season for all participants. Of the participating HCWs, 44.0% were positive tested by the Flu SensDx device. There were no statistically significant differences among the positive tested HCWs, their influenza immunization history, and the presence of symptoms of influenza-like illness (p &gt; 0.05). Conclusion: Although the results of the present study suggest that influenza vaccination does not reduce the frequency of influenza virus detection by Flu SensDx testing in the HCWs participants, larger studies are needed to estimate the incidence of influenza virus infection among HCWs to understand the underlying mechanism and fine-tune policies aimed at reducing nosocomial infections.<\/jats:p>","DOI":"10.3390\/ijerph19063159","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T12:35:37Z","timestamp":1646742937000},"page":"3159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Incidence of Influenza Virus Infection among Wroclaw\u2019s Healthcare Workers in Pre-COVID-19 2019\u20132020 Influenza Season Using Novel Flu SensDx Device"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1964-4044","authenticated-orcid":false,"given":"Micha\u0142 Jacek","family":"J\u0119drzejek","sequence":"first","affiliation":[{"name":"Department of Family Medicine, Wroclaw Medical University, W. Syrokomli 1, 51-141 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6600-2760","authenticated-orcid":false,"given":"Agnieszka","family":"Mastalerz-Migas","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, Wroclaw Medical University, W. Syrokomli 1, 51-141 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"Janicka","sequence":"additional","affiliation":[{"name":"Department of Pathology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.5114\/fmpcr.2020.90629","article-title":"Recommendations for the prevention, diagnosis and treatment of inFLUenza in adults for Primary care physiciAnS: FLU COMPAS PCP\u2013ADULTS","volume":"22","author":"Kuchar","year":"2020","journal-title":"Fam. Med. Prim. Care Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1007\/s10096-014-2119-6","article-title":"Healthcare workers as vectors of infectious diseases","volume":"33","author":"Huttunen","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100036","DOI":"10.1016\/j.jvacx.2019.100036","article-title":"A rapid evidence appraisal of influenza vaccination in health workers: An important policy in an area of imperfect evidence","volume":"2","author":"Jenkin","year":"2019","journal-title":"Vaccine X"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kuster, S., Shah, P.S., Coleman, B.L., Lam, P.-P., Tong, A., Wormsbecker, A., and McGeer, A. (2011). Incidence of Influenza in Healthy Adults and Healthcare Workers: A Systematic Review and Meta-Analysis. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026239"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1136\/bmj.313.7067.1241","article-title":"Incidence and recall of influenza in a cohort of Glasgow healthcare workers during the 1993\u20134 epidemic: Results of serum testing and questionnaire","volume":"313","author":"Elder","year":"1996","journal-title":"BMJ"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Sandoval, C., Barrera, A., Ferr\u00e9s, M., Cerda, J., Retamal, J., Garcia-Sastre, A., Medina, R.A., and Hirsch, T. (2016). Infection in Health Personnel with High and Low Levels of Exposure in a Hospital Setting during the H1N1 2009 Influenza A Pandemic. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0147271"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/0196-6553(89)90129-6","article-title":"Barriers to influenza vaccine acceptance A survey of physicians and nurses","volume":"17","author":"Weingarten","year":"1989","journal-title":"Am. J. Infect. Control"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"ofz096","DOI":"10.1093\/ofid\/ofz096","article-title":"Universal Influenza Vaccination Among Healthcare Personnel: Yes We Should","volume":"6","author":"Perl","year":"2019","journal-title":"Open Forum Infect. Dis."},{"key":"ref_9","first-page":"225","article-title":"Influenza vaccination for health care workers: A duty of care","volume":"11","author":"Orr","year":"2000","journal-title":"Can. J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1007\/s11606-010-1422-x","article-title":"Presenteeism: A Public Health Hazard","volume":"25","author":"Widera","year":"2010","journal-title":"J. Gen. Intern. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jhin.2008.08.013","article-title":"Hospital-acquired influenza: A synthesis using the Outbreak Reports and Intervention Studies of Nosocomial Infection (ORION) statement","volume":"71","author":"Voirin","year":"2009","journal-title":"J. Hosp. Infect."},{"key":"ref_12","unstructured":"World Health Organization (2010). Limiting Spread. Limiting the Spread of Pandemic, Zoonotic, and Seasonal Epidemic Influenza, World Health Organization."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.puhe.2017.10.027","article-title":"Self-reported influenza vaccination rates and attitudes towards vaccination among health care workers: Results of a survey in a German university hospital","volume":"154","author":"Hagemeister","year":"2018","journal-title":"Public Health"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1386-6532(03)00122-7","article-title":"Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement","volume":"29","author":"Ward","year":"2003","journal-title":"J. Clin. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1093\/infdis\/jis450","article-title":"Longitudinal Study of Influenza Molecular Viral Shedding in Hutterite Communities","volume":"206","author":"Loeb","year":"2012","journal-title":"J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1086\/669857","article-title":"Respiratory Virus Shedding in a Cohort of On-Duty Healthcare Workers Undergoing Prospective Surveillance","volume":"34","author":"Esbenshade","year":"2013","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.3201\/eid2206.151080","article-title":"Heterogeneous and Dynamic Prevalence of Asymptomatic Influenza Virus Infections","volume":"22","author":"Cox","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1177\/003335490912400205","article-title":"Does Influenza Transmission Occur from Asymptomatic Infection or Prior to Symptom Onset?","volume":"124","author":"Patrozou","year":"2009","journal-title":"Public Health Rep."},{"key":"ref_19","first-page":"736","article-title":"Viral shedding and transmission potential of asymptomatic and pauci-symptomatic influenza virus infections in the community","volume":"64","author":"Ip","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1086\/674858","article-title":"Healthcare-Associated Influenza in Canadian Hospitals from 2006 to 2012","volume":"35","author":"Taylor","year":"2014","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1097\/EDE.0000000000000340","article-title":"The fraction of influenza virus infections that are asymptomatic: A systematic review and meta-analysis","volume":"26","author":"Leung","year":"2015","journal-title":"Epidemiology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1097\/EDE.0b013e3181e94274","article-title":"Estimating Pathogen-specific Asymptomatic Ratios","volume":"21","author":"Wang","year":"2010","journal-title":"Epidemiology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1093\/aje\/kwm375","article-title":"Time Lines of Infection and Disease in Human Influenza: A Review of Volunteer Challenge Studies","volume":"167","author":"Carrat","year":"2008","journal-title":"Am. J. Epidemiol."},{"key":"ref_24","first-page":"431","article-title":"The Dynamic Relationship Between Clinical Symptomatology and Viral Shedding in Naturally Acquired Seasonal and Pandemic Influenza Virus Infections","volume":"62","author":"Ip","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1086\/652241","article-title":"Viral Shedding and Clinical Illness in Naturally Acquired Influenza Virus Infections","volume":"201","author":"Lau","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1093\/cid\/civ163","article-title":"Influenza Among Afebrile and Vaccinated Healthcare Workers","volume":"60","author":"Ridgway","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"ref_27","first-page":"CD006207","article-title":"Physical interventions to interrupt or reduce the spread of respiratory viruses","volume":"11","author":"Jefferson","year":"2020","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_28","unstructured":"(2021, December 20). NIPH-NIH NI of PH-NI of H. Available online: http:\/\/wwwold.pzh.gov.pl\/oldpage\/epimeld\/grypa\/aindex.htm."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Caini, S., Kusznierz, G., Garate, V.V., Wangchuk, S., Thapa, B., J\u00fanior, F.J.D.P., De Almeida, W.A.F., Njouom, R., Fasce, R.A., and Bustos, P. (2019). The epidemiological signature of influenza B virus and its B\/Victoria and B\/Yamagata lineages in the 21st century. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0222381"},{"key":"ref_30","first-page":"115","article-title":"Epidemic Influenza Seasons from 2008 to 2018 in Poland: A Focused Review of Virological Characteristics","volume":"1251","author":"Byambasuren","year":"2020","journal-title":"Retin. Degener. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/j.1750-2659.2011.00240.x","article-title":"Vaccination of health-care workers against influenza: Our obligation to protect patients","volume":"5","author":"Maltezou","year":"2011","journal-title":"Influ. Other Respir. Viruses"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1001\/jama.281.10.908","article-title":"Effectiveness of influenza vaccine in health care professionals: A randomized trial","volume":"281","author":"Wilde","year":"1999","journal-title":"JAMA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e012149","DOI":"10.1136\/bmjopen-2016-012149","article-title":"Influenza vaccination for healthcare workers in the UK: Appraisal of systematic reviews and policy options","volume":"6","author":"Kliner","year":"2016","journal-title":"BMJ Open"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/s41577-019-0143-6","article-title":"The human antibody response to influenza A virus infection and vaccination","volume":"19","author":"Krammer","year":"2019","journal-title":"Nat. Rev. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.vaccine.2020.12.018","article-title":"Global production capacity of seasonal and pandemic influenza vaccines in 2019","volume":"39","author":"Sparrow","year":"2021","journal-title":"Vaccine"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2803","DOI":"10.1128\/JVI.02424-08","article-title":"Blocking Interhost Transmission of Influenza Virus by Vaccination in the Guinea Pig Model","volume":"83","author":"Lowen","year":"2009","journal-title":"J. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e241087","DOI":"10.1136\/bcr-2020-241087","article-title":"Persistent positivity of SARS-CoV-2 nucleic acid in asymptomatic healthcare worker: Infective virion or inactive nucleic acid?","volume":"14","author":"Tiwari","year":"2021","journal-title":"BMJ Case Rep."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/6\/3159\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:33:44Z","timestamp":1760135624000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/6\/3159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,8]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["ijerph19063159"],"URL":"https:\/\/doi.org\/10.3390\/ijerph19063159","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,8]]}}}