{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:40:55Z","timestamp":1791895255541,"version":"4.3.4"},"reference-count":110,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T00:00:00Z","timestamp":1656892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006261","name":"Taif University Researchers Supporting Project","doi-asserted-by":"publisher","award":["TURSP-2020\/152"],"award-info":[{"award-number":["TURSP-2020\/152"]}],"id":[{"id":"10.13039\/501100006261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The nano-sized iron oxide (Fe2O3-NPs) is one of the most used engineered nanomaterials worldwide. This study investigated the efficacy of natural polyphenol resveratrol (RSV) (20 mg\/kg b.wt, orally once daily) to alleviate the impaired sperm quality and testicular injury resulting from Fe2O3-NPs exposure (3.5 or 7 mg\/kg b.wt, intraperitoneally once a week) for eight weeks. Spermiograms, sexual hormonal levels, oxidative stress indicators, and lipid peroxidation biomarker were assessed. Moreover, the steroidogenesis-related genes mRNA expressions were evaluated. The results showed that RSV substantially rescued Fe2O3-NPs-mediated sperm defects. Additionally, the Fe2O3-NPs-induced depressing effects on sperm motility and viability were markedly counteracted by RSV. Moreover, RSV significantly restored Fe2O3-NPs-induced depletion of testosterone, follicle-stimulated hormone, luteinizing hormone, and testicular antioxidant enzymes but reduced malondialdehyde content. Furthermore, the Fe2O3-NPs-induced downregulation of steroidogenesis-related genes (3 \u03b2-HSD, 17 \u03b2-HSD, and Nr5A1) was significantly counteracted in the testicular tissue of RSV-treated rats. These findings concluded that RSV could limit the Fe2O3-NPs-induced reduced sperm quality and testicular injury most likely via their antioxidant activity and steroidogenesis-related gene expression modulation.<\/jats:p>","DOI":"10.3390\/ijerph19138171","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T11:15:05Z","timestamp":1656933305000},"page":"8171","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Palliative Effect of Resveratrol against Nanosized Iron Oxide-Induced Oxidative Stress and Steroidogenesis-Related Genes Dysregulation in Testicular Tissue of Adult Male Rats"],"prefix":"10.3390","volume":"19","author":[{"given":"Mona M.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 4511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5322-3860","authenticated-orcid":false,"given":"Mohamed M. A.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 4511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisir","family":"Saber","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3646-6385","authenticated-orcid":false,"given":"Yasmina M.","family":"Abd-Elhakim","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 4511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21073","DOI":"10.1038\/srep21073","article-title":"Optimization of a nanotechnology based antimicrobial platform for food safety applications using Engineered Water Nanostructures (EWNS)","volume":"6","author":"Pyrgiotakis","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Abd-Elhakim, Y.M., Hashem, M.M., Abo-EL-Sooud, K., Hassan, B.A., Elbohi, K.M., and Al-Sagheer, A.A. (2021). Effects of Co-Exposure of Nanoparticles and Metals on Different Organisms: A Review. Toxics, 9.","DOI":"10.3390\/toxics9110284"},{"key":"ref_3","unstructured":"ECHA (2022, April 12). European Union Observatory for Nanomaterial: Catalogue of Cosmetic Ingredients. Available online: https:\/\/euon.echa.europa.eu\/catalogue-ofcosmetic-ingredients."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1021\/mp900083m","article-title":"Cell uptake and in vitro toxicity of magnetic nanoparticles suitable for drug delivery","volume":"6","author":"Riffle","year":"2009","journal-title":"Mol. Pharm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1016\/j.jmmm.2008.12.017","article-title":"Size-dependant heating rates of iron oxide nanoparticles for magnetic fluid hyperthermia","volume":"321","author":"Zeisberger","year":"2009","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.2174\/092986709787846604","article-title":"Iron oxide nanoparticle platform for biomedical applications","volume":"16","author":"Xie","year":"2009","journal-title":"Curr. Med. Chem."},{"key":"ref_7","first-page":"4","article-title":"Temperature controlled CO2 laser welding of soft tissues: Urinary bladder welding in different animal models (rats, rabbits, and cats)","volume":"26","author":"Lobel","year":"2000","journal-title":"Lasers Surg. Med. Off. J. Am. Soc. Laser Med. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"983","DOI":"10.2147\/IJN.S13244","article-title":"Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress","volume":"5","author":"Naqvi","year":"2010","journal-title":"Int. J. Nanomed."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.taap.2012.10.016","article-title":"Comparative study of genotoxicity and tissue distribution of nano and micron sized iron oxide in rats after acute oral treatment","volume":"266","author":"Singh","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_10","first-page":"373","article-title":"Toxic effects of the Fe2O3 nanoparticles on the liver and lung tissue","volume":"116","author":"Sadeghi","year":"2015","journal-title":"Bratisl. Lek. Listy"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jtemb.2018.06.006","article-title":"Sub-acute intravenous exposure to Fe2O3 nanoparticles does not alter cognitive performances and catecholamine levels, but slightly disrupts plasma iron level and brain iron content in rats","volume":"50","author":"Askri","year":"2018","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"103303","DOI":"10.1016\/j.etap.2019.103303","article-title":"Pulmonary toxicity of Fe2O3, ZnFe2O4, NiFe2O4 and NiZnFe4O8 nanomaterials: Inflammation and DNA strand breaks","volume":"74","author":"Hadrup","year":"2020","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1002\/jat.3485","article-title":"Iron oxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in lymphocytes","volume":"37","author":"Gaharwar","year":"2017","journal-title":"J. Appl. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yarjanli, Z., Ghaedi, K., Esmaeili, A., Rahgozar, S., and Zarrabi, A. (2017). Iron oxide nanoparticles may damage to the neural tissue through iron accumulation, oxidative stress, and protein aggregation. BMC Neurosci., 18.","DOI":"10.1186\/s12868-017-0369-9"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1002\/tox.22262","article-title":"Repeated exposure to iron oxide nanoparticles causes testicular toxicity in mice","volume":"32","author":"Sundarraj","year":"2017","journal-title":"Environ. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Dora, M.F., Taha, N.M., Lebda, M.A., Hashem, A.E., Elfeky, M.S., El-Sayed, Y.S., Jaouni, S.A., and El-Far, A.H. (2021). Quercetin Attenuates Brain Oxidative Alterations Induced by Iron Oxide Nanoparticles in Rats. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22083829"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.taap.2017.01.002","article-title":"Iron oxide nanoparticles modulate heat shock proteins and organ specific markers expression in mice male accessory organs","volume":"317","author":"Sundarraj","year":"2017","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"39","DOI":"10.32598\/IJT.12.6.490.1","article-title":"Effects of Iron Oxide Nanoparticles on Mouse Sperm Parameters and Testicular Tissue","volume":"12","author":"Parivar","year":"2018","journal-title":"Iran. J. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"117782","DOI":"10.1016\/j.lfs.2020.117782","article-title":"Taurine and hesperidin rescues carbon tetrachloride-triggered testicular and kidney damage in rat via modulating oxidative stress and inflammation","volume":"254","author":"Ghoneim","year":"2020","journal-title":"Life Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ecoenv.2018.05.073","article-title":"Spirulina platensis attenuates furan reprotoxicity by regulating oxidative stress, inflammation, and apoptosis in testis of rats","volume":"161","author":"Mohamed","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Behairy, A., El-Sharkawy, N.I., Saber, T.M., Soliman, M.M., Metwally, M.M.M., Abd El-Rahman, G.I., Abd-Elhakim, Y.M., and El Deib, M.M. (2020). The Modulatory Role of Vitamin C in Boldenone Undecylenate Induced Testicular Oxidative Damage and Androgen Receptor Dysregulation in Adult Male Rats. Antioxidants, 9.","DOI":"10.3390\/antiox9111053"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.cbi.2008.07.009","article-title":"Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress","volume":"176","author":"Sahoo","year":"2008","journal-title":"Chem. Biol. Interact."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.ecoenv.2018.11.082","article-title":"Food Yellow4 reprotoxicity in relation to localization of DMC1 and apoptosis in rat testes: Roles of royal jelly and cod liver oil","volume":"169","author":"Elewa","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Salehi, B., Mishra, A.P., Nigam, M., Sener, B., Kilic, M., Sharifi-Rad, M., Fokou, P.V.T., Martins, N., and Sharifi-Rad, J. (2018). Resveratrol: A Double-Edged Sword in Health Benefits. Biomedicines, 6.","DOI":"10.3390\/biomedicines6030091"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.foodchem.2018.03.085","article-title":"Antioxidant activity of resveratrol ester derivatives in food and biological model systems","volume":"261","author":"Oh","year":"2018","journal-title":"Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.reprotox.2020.05.005","article-title":"The protective effects of pretreatment with resveratrol in cyclophosphamide-induced rat ovarian granulosa cell injury: In vitro study","volume":"95","author":"Nie","year":"2020","journal-title":"Reprod. Toxicol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.cyto.2018.10.008","article-title":"Protective mechanisms of resveratrol derivatives against TNF-\u03b1-induced inflammatory responses in rat mesangial cells","volume":"113","author":"Lee","year":"2019","journal-title":"Cytokine"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.biopha.2018.11.075","article-title":"Resveratrol: From enhanced biosynthesis and bioavailability to multitargeting chronic diseases","volume":"109","author":"Pannu","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.fct.2013.07.021","article-title":"Antioxidant effects of resveratrol in cardiovascular, cerebral and metabolic diseases","volume":"61","author":"Carrizzo","year":"2013","journal-title":"Food Chem. Toxicol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6505","DOI":"10.1007\/s11356-019-07344-8","article-title":"Synergistic antioxidant effects of resveratrol and curcumin against fipronil-triggered oxidative damage in male albino rats","volume":"27","author":"AlBasher","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1111\/bph.13492","article-title":"Antioxidant effects of resveratrol in the cardiovascular system","volume":"174","author":"Xia","year":"2017","journal-title":"Br. J. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"107613","DOI":"10.1016\/j.pharmthera.2020.107613","article-title":"Therapeutic potential of resveratrol against emerging respiratory viral infections","volume":"214","author":"Filardo","year":"2020","journal-title":"Pharmacol. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.3389\/fphar.2020.619024","article-title":"Resveratrol and Neuroprotection: Impact and Its Therapeutic Potential in Alzheimer\u2019s Disease","volume":"11","author":"Rahman","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"117727","DOI":"10.1016\/j.lfs.2020.117727","article-title":"NLRP3 inflammasome drives inflammation in high fructose fed diabetic rat liver: Effect of resveratrol and metformin","volume":"253","author":"Rai","year":"2020","journal-title":"Life Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.amjsurg.2020.02.025","article-title":"Histomorphometric evaluation of the rat kidney submitted to warm ischemia and the protective effect of resveratrol","volume":"220","author":"Sampaio","year":"2020","journal-title":"Am. J. Surg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"489","DOI":"10.3390\/nu6020489","article-title":"Protective effect of resveratrol on biomarkers of oxidative stress induced by iron\/ascorbate in mouse spermatozoa","volume":"6","year":"2014","journal-title":"Nutrients"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/and.12301","article-title":"Lycopene and resveratrol improve post-thaw bull sperm parameters: Sperm motility, mitochondrial activity and DNA integrity","volume":"47","author":"Bucak","year":"2015","journal-title":"Andrologia"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.reprotox.2010.11.010","article-title":"Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells","volume":"31","author":"Collodel","year":"2011","journal-title":"Reprod. Toxicol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0027-5107(95)00223-5","article-title":"Comparison of the duration of spermatogenesis between male rodents and humans","volume":"352","author":"Adler","year":"1996","journal-title":"Mutat. Res. \/Fundam. Mol. Mech. Mutagenesis"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1262\/jrd.2013-097","article-title":"Resveratrol reverses cadmium chloride-induced testicular damage and subfertility by downregulating p53 and Bax and upregulating gonadotropins and Bcl-2 gene expression","volume":"60","author":"Eleawa","year":"2014","journal-title":"J. Reprod. Dev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"99","DOI":"10.4103\/0974-1208.138867","article-title":"Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol","volume":"7","author":"Sharma","year":"2014","journal-title":"J. Hum. Reprod. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1097\/00029330-200807010-00010","article-title":"Resveratrol reestablishes spermatogenesis after testicular injury in rats caused by 2, 5-hexanedione","volume":"121","author":"Jiang","year":"2008","journal-title":"Chin. Med. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/jn\/132.2.257","article-title":"The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful","volume":"132","author":"Juan","year":"2002","journal-title":"J. Nutr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1093\/toxsci\/kfj027","article-title":"Toxicity and tissue distribution of magnetic nanoparticles in mice","volume":"89","author":"Kim","year":"2006","journal-title":"Toxicol. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1166\/jnn.2012.5796","article-title":"Repeated Oral Dose Toxicity of Iron Oxide Nanoparticles: Biochemical and Histopathological Alterations in Different Tissues of Rats","volume":"12","author":"Kumari","year":"2012","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Askri, D., Cunin, V., Ouni, S., B\u00e9al, D., Rachidi, W., Sakly, M., Amara, S., Lehmann, S.G., and S\u00e8ve, M. (2019). Effects of Iron Oxide Nanoparticles (\u03b3-Fe2O3) on Liver, Lung and Brain Proteomes following Sub-Acute Intranasal Exposure: A New Toxicological Assessment in Rat Model Using iTRAQ-Based Quantitative Proteomics. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20205186"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s12640-017-9721-1","article-title":"Neurobehavioural Toxicity of Iron Oxide Nanoparticles in Mice","volume":"32","author":"Dhakshinamoorthy","year":"2017","journal-title":"Neurotox. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2995","DOI":"10.3748\/wjg.15.2995","article-title":"Nanosized As2O3\/Fe2O3 complexes combined with magnetic fluid hyperthermia selectively target liver cancer cells","volume":"15","author":"Wang","year":"2009","journal-title":"World J. Gastroenterol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.jmmm.2004.11.131","article-title":"Genotoxicity and inflammatory investigation in mice treated with magnetite nanoparticles surface coated with polyaspartic acid","volume":"289","author":"Sadeghiani","year":"2005","journal-title":"J. Magn. Magn. Mater."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Pham, B.T., Colvin, E.K., Pham, N.T., Kim, B.J., Fuller, E.S., Moon, E.A., Barbey, R., Yuen, S., Rickman, B.H., and Bryce, N.S. (2018). Biodistribution and clearance of stable superparamagnetic maghemite iron oxide nanoparticles in mice following intraperitoneal administration. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19010205"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"587021","DOI":"10.1155\/2013\/587021","article-title":"Iron Oxide Magnetic Nanoparticles: Characterization and Toxicity Evaluation by In Vitro and In Vivo Assays","volume":"2013","author":"Prodan","year":"2013","journal-title":"J. Nanomater."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"53","DOI":"10.21608\/jva.2009.42311","article-title":"Characterizing the gonadosomatic index and its relationship with age in greater cane rat (Thryonomys swinderianus, Temminck)","volume":"2","author":"Adebayo","year":"2009","journal-title":"J. Vet. Anat."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/0890-6238(91)90009-5","article-title":"Rat sperm motility analysis: Methodologic considerations","volume":"5","author":"Slott","year":"1991","journal-title":"Reprod. Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1590\/S1807-59322009000600011","article-title":"Radio frequency electromagnetic radiation (RF-EMR) from GSM (0.9\/1.8 GHz) mobile phones induces oxidative stress and reduces sperm motility in rats","volume":"64","author":"Mailankot","year":"2009","journal-title":"Clinics"},{"key":"ref_55","unstructured":"Chapin, R.E., and Heildel, J.J. (1993). Methods for evaluation of rat epididymal sperm morphology. Methods in Toxicology: Male Reproductive Toxicology, Elsevier."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/S0006-291X(72)80218-3","article-title":"The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen","volume":"46","author":"Nishikimi","year":"1972","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"[13] Catalase in vitro","volume":"Volume 105","author":"Aebi","year":"1984","journal-title":"Methods in Enzymology"},{"key":"ref_58","first-page":"158","article-title":"Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase","volume":"70","author":"Paglia","year":"1967","journal-title":"J. Lab. Clin. Med."},{"key":"ref_59","first-page":"882","article-title":"Improved method for the determination of blood glutathione","volume":"61","author":"Beutler","year":"1963","journal-title":"J. Lab. Clin. Med."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.biopha.2017.04.026","article-title":"Neurotoxic effects of silver nanoparticles and the protective role of rutin","volume":"90","author":"Ahmed","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212 \u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"161","DOI":"10.3109\/15376516.2013.764950","article-title":"Toxic effects of repeated oral exposure of silver nanoparticles on small intestine mucosa of mice","volume":"23","author":"Shahare","year":"2013","journal-title":"Toxicol. Mech. Methods"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Xu, J., Shi, H., Ruth, M., Yu, H., Lazar, L., Zou, B., Yang, C., Wu, A., and Zhao, J. (2013). Acute toxicity of intravenously administered titanium dioxide nanoparticles in mice. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0070618"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1530\/EJE-10-0538","article-title":"Effect of weight reduction on insulin sensitivity, sex hormone-binding globulin, sex hormones and gonadotrophins in obese children","volume":"163","author":"Birkebaek","year":"2010","journal-title":"Eur. J. Endocrinol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ejphar.2014.05.005","article-title":"Diabetes-induced DNA damage and apoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis","volume":"737","author":"Kilarkaje","year":"2014","journal-title":"Eur. J. Pharmacol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.taap.2015.10.012","article-title":"Resveratrol alleviates diabetes-induced testicular dysfunction by inhibiting oxidative stress and c-Jun N-terminal kinase signaling in rats","volume":"289","author":"Faid","year":"2015","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12302-021-00585-w","article-title":"Impact of environmental factors on human semen quality and male fertility: A narrative review","volume":"34","author":"Kumar","year":"2022","journal-title":"Environ. Sci. Eur."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S0010-7824(00)00177-3","article-title":"The reversible antifertility effect of Piper betle Linn. on Swiss albino male mice","volume":"62","author":"Sarkar","year":"2000","journal-title":"Contraception"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3109\/01485019308988370","article-title":"Hyperviscosity and hypofunction of the seminal vesicles","volume":"30","author":"Gonzales","year":"1993","journal-title":"Arch. Androl."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1002\/cbin.10451","article-title":"Resveratrol in spermatogenesis","volume":"39","author":"Chirumbolo","year":"2015","journal-title":"Cell Biol. Int."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1002\/cbin.10441","article-title":"Effect of resveratrol on restoring spermatogenesis in experimental cryptorchid mice and analysis of related differentially expressed proteins","volume":"39","author":"Li","year":"2015","journal-title":"Cell Biol. Int."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jnutbio.2016.04.003","article-title":"Resveratrol ameliorates benzo (a) pyrene-induced testicular dysfunction and apoptosis: Involvement of p38 MAPK\/ATF2\/iNOS signaling","volume":"34","author":"Banerjee","year":"2016","journal-title":"J. Nutr. Biochem."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.etp.2014.12.002","article-title":"Effects of resveratrol on doxorubicin induced testicular damage in rats","volume":"67","author":"Alver","year":"2015","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e13978","DOI":"10.1111\/and.13978","article-title":"Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic-and spermatogenic-related genes in immature male albino rats","volume":"53","author":"Hassan","year":"2021","journal-title":"Andrologia"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10715762.2019.1702656","article-title":"Hormones and oxidative stress: An overview","volume":"54","author":"Chainy","year":"2020","journal-title":"Free Radic. Res."},{"key":"ref_77","unstructured":"Henkel, R., Samanta, L., and Agarwal, A. (2019). Chapter 2.7\u2014Thyroid Dysfunction and Testicular Redox Status: An Intriguing Association. Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction, Academic Press."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"335","DOI":"10.4103\/0976-9668.159998","article-title":"Toxic effects of Mn2O3 nanoparticles on rat testis and sex hormone","volume":"6","author":"Negahdary","year":"2015","journal-title":"J. Nat. Sci. Biol. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1093\/jn\/135.4.757","article-title":"trans-Resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats","volume":"135","author":"Juan","year":"2005","journal-title":"J. Nutr."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1002\/chir.20007","article-title":"Differential recognition of resveratrol isomers by the human estrogen receptor-\u03b1: Molecular dynamics evidence for stereoselective ligand binding","volume":"16","year":"2004","journal-title":"Chirality"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1021\/cr2002596","article-title":"Assessing the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles","volume":"112","author":"Mahmoudi","year":"2012","journal-title":"Chem. Rev."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.fct.2003.10.005","article-title":"Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol","volume":"42","author":"Jurczuk","year":"2004","journal-title":"Food Chem. Toxicol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1007\/s00244-009-9371-2","article-title":"Changes in sperm characteristics and induction of oxidative stress in the testis and epididymis of experimental rats by a herbicide, atrazine","volume":"58","author":"Abarikwu","year":"2010","journal-title":"Arch. Environ. Contam. Toxicol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1148\/radiol.11101139","article-title":"Pilot pharmacokinetic and dosimetric studies of 18F-FPPRGD2: A PET radiopharmaceutical agent for imaging \u03b1v\u03b23 integrin levels","volume":"260","author":"Mittra","year":"2011","journal-title":"Radiology"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1016\/j.jnutbio.2015.07.008","article-title":"Effect of excess iron on oxidative stress and gluconeogenesis through hepcidin during mitochondrial dysfunction","volume":"26","author":"Lee","year":"2015","journal-title":"J. Nutr. Biochem."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"118535","DOI":"10.1016\/j.bbamcr.2019.118535","article-title":"Iron homeostasis and oxidative stress: An intimate relationship","volume":"1866","author":"Galaris","year":"2019","journal-title":"Biochim. Biophys. Acta BBA Mol. Cell Res."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Aitken, R.J., and Roman, S.D. (2009). Antioxidant systems and oxidative stress in the testes. Molecular Mechanisms in Spermatogenesis, Springer.","DOI":"10.1007\/978-0-387-09597-4_9"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.niox.2011.12.006","article-title":"Cardiovascular effects and molecular targets of resveratrol","volume":"26","author":"Li","year":"2012","journal-title":"Nitric Oxide"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"22586","DOI":"10.1074\/jbc.M101846200","article-title":"Specific structural determinants are responsible for the antioxidant activity and the cell cycle effects of resveratrol","volume":"276","author":"Stivala","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1007\/s11095-010-0090-1","article-title":"Resveratrol and resveratrol analogues\u2014Structure\u2014Activity relationship","volume":"27","author":"Szekeres","year":"2010","journal-title":"Pharm. Res."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"3868","DOI":"10.1021\/jo3002134","article-title":"Antioxidant activity of trans-resveratrol toward hydroxyl and hydroperoxyl radicals: A quantum chemical and computation al kinetics study","volume":"77","author":"Iuga","year":"2012","journal-title":"J. Org. Chem."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"401","DOI":"10.2174\/1389201015666140711114450","article-title":"Resveratrol as a gene regulator in the vasculature","volume":"15","author":"Xia","year":"2014","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s11010-008-9756-y","article-title":"EPR studies on the superoxide-scavenging capacity of the nutraceutical resveratrol","volume":"313","author":"Jia","year":"2008","journal-title":"Mol. Cell. Biochem."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"H1876","DOI":"10.1152\/ajpheart.00375.2009","article-title":"Resveratrol attenuates mitochondrial oxidative stress in coronary arterial endothelial cells","volume":"297","author":"Ungvari","year":"2009","journal-title":"Am. J. Physiol.\u2014Heart Circ. Physiol."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Bremer, A., and Miller, W. (2014). Chapter 13-Regulation of Steroidogenesis. Cellular Endocrinology in Health and Disease, Elsevier.","DOI":"10.1016\/B978-0-12-408134-5.00013-5"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"56491","DOI":"10.1007\/s11356-021-14339-x","article-title":"Iprodione and\/or chlorpyrifos exposure induced testicular toxicity in adult rats by suppression of steroidogenic genes and SIRT1\/TERT\/PGC-1\u03b1 pathway","volume":"28","author":"Hassan","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Abd El-Hakim, Y.M., Abdel-Rahman Mohamed, A., Khater, S.I., Hamed Arisha, A., Metwally, M.M., Nassan, M.A., and Hassan, M.E. (2020). Chitosan-stabilized selenium nanoparticles and metformin synergistically rescue testicular oxidative damage and steroidogenesis-related genes dysregulation in high-fat diet\/streptozotocin-induced diabetic rats. Antioxidants, 10.","DOI":"10.3390\/antiox10010017"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12951-019-0474-2","article-title":"SF-1 mediates reproductive toxicity induced by Cerium oxide nanoparticles in male mice","volume":"17","author":"Qin","year":"2019","journal-title":"J. Nanobiotechnol."},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Vassal, M., Rebelo, S., and Pereira, M.d.L. (2021). Metal Oxide Nanoparticles: Evidence of Adverse Effects on the Male Reproductive System. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22158061"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/jbt.21812","article-title":"Querectin alleviates zinc oxide nanoreprotoxicity in male albino rats","volume":"30","author":"Hussein","year":"2016","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"229","DOI":"10.30574\/gscbps.2020.12.2.0272","article-title":"Changes in semen characteristics and sex hormones of rats treated with iron oxide nanoparticles, silver nanoparticles and their mixture","volume":"12","author":"Ahmed","year":"2020","journal-title":"GSC Biol. Pharm. Sci."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1177\/1933719112459241","article-title":"A phytooxysterol, 28-homobrassinolide modulates rat testicular steroidogenesis in normal and diabetic rats","volume":"20","author":"Premalatha","year":"2013","journal-title":"Reprod. Sci."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/j.freeradbiomed.2009.09.004","article-title":"ROS inhibit the expression of testicular steroidogenic enzyme genes via the suppression of Nur77 transactivation","volume":"47","author":"Lee","year":"2009","journal-title":"Free Radic. Biol. Med."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1002\/jcb.10738","article-title":"Antisteroidogenic actions of hydrogen peroxide on rat Leydig cells","volume":"90","author":"Tsai","year":"2003","journal-title":"J. Cell. Biochem."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1210\/endo.133.6.8243310","article-title":"The effects of hydrogen peroxide on steroidogenesis in mouse Leydig tumor cells","volume":"133","author":"Stocco","year":"1993","journal-title":"Endocrinology"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"488","DOI":"10.2164\/jandrol.108.005132","article-title":"Oxidative stress: A common factor in testicular dysfunction","volume":"29","author":"Turner","year":"2008","journal-title":"J. Androl."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1677\/JOE-07-0570","article-title":"Oxidative stress-induced inhibition of adrenal steroidogenesis requires participation of p38 mitogen-activated protein kinase signaling pathway","volume":"198","author":"Abidi","year":"2008","journal-title":"J. Endocrinol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1210\/endo.137.1.8536600","article-title":"Induction of lipid peroxidation during steroidogenesis in the rat testis","volume":"137","author":"Peltola","year":"1996","journal-title":"Endocrinology"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1111\/and.12478","article-title":"Antioxidant enzymes activity, lipid peroxidation, oxidative damage in the testis and epididymis, and steroidogenesis in rats after co-exposure to atrazine and ethanol","volume":"48","author":"Abarikwu","year":"2016","journal-title":"Andrologia"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3389\/fendo.2019.00272","article-title":"Protective Effect of Resveratrol on Benzo(a)Pyrene Induced Dysfunctions of Steroidogenesis and Steroidogenic Acute Regulatory Gene Expression in Leydig Cells","volume":"10","author":"Banerjee","year":"2019","journal-title":"Front. Endocrinol."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/13\/8171\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:42:24Z","timestamp":1760139744000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/13\/8171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,4]]},"references-count":110,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["ijerph19138171"],"URL":"https:\/\/doi.org\/10.3390\/ijerph19138171","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,4]]}}}