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Therefore, regulation of dopamine transporter (DAT1) gene expression is critical for maintaining homeostasis in the dopaminergic system. The aim of our study is evaluation of the methylation status of 33 CpG islands located in the DAT1 gene promoter region related to nicotine dependency. We investigated 142 nicotine-dependent subjects and 238 controls. Our results show that as many as 14 of the 33 CpG islands tested had statistically significantly higher methylation in the nicotine-dependent group compared to the control group. After applying Bonferroni correction, the total number of methylation sites was also significantly higher in the dependent subjects group. The analysis of the methylation status of particular CpG sites revealed a new direction of research regarding the biological aspects of nicotine addiction.<\/jats:p>","DOI":"10.3390\/ijerph19148602","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:17:10Z","timestamp":1657844230000},"page":"8602","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Methylation in the Promoter Region of the Dopamine Transporter DAT1 Gene in People Addicted to Nicotine"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3285-5313","authenticated-orcid":false,"given":"Jolanta","family":"Chmielowiec","sequence":"first","affiliation":[{"name":"Department of Hygiene and Epidemiology, Collegium Medicum, University of Zielona G\u00f3ra, 65-046 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4254-5466","authenticated-orcid":false,"given":"Krzysztof","family":"Chmielowiec","sequence":"additional","affiliation":[{"name":"Department of Hygiene and Epidemiology, Collegium Medicum, University of Zielona G\u00f3ra, 65-046 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6564-2141","authenticated-orcid":false,"given":"Aleksandra","family":"Stro\u0144ska-Pluta","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-1429","authenticated-orcid":false,"given":"Aleksandra","family":"Suchanecka","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0034-0809","authenticated-orcid":false,"given":"Kinga","family":"Humi\u0144ska-Lisowska","sequence":"additional","affiliation":[{"name":"Faculty of Physical Education, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8402-0564","authenticated-orcid":false,"given":"Milena","family":"Lachowicz","sequence":"additional","affiliation":[{"name":"Department of Psychology, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Niewczas","sequence":"additional","affiliation":[{"name":"Faculty of Physical Education, University of Rzeszow, 35-959 Rzeszow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Bia\u0142ecka","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Therapeutic Drug Monitoring, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"\u015amiarowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Therapeutic Drug Monitoring, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2633-520X","authenticated-orcid":false,"given":"Anna","family":"Grzywacz","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,14]]},"reference":[{"key":"ref_1","unstructured":"National Center for Chronic Disease Prevention and Health Promotion (US) Office on Smoking and Health (2014). 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