{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T19:42:19Z","timestamp":1788637339174,"version":"build-2803163510"},"reference-count":73,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T00:00:00Z","timestamp":1661212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minister of Science and Higher Education under the name \u201cRegional Initiative of Excellence\u201d in 2019\u20132022","award":["project number 002\/RID\/2018\/19"],"award-info":[{"award-number":["project number 002\/RID\/2018\/19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Smoking is a chronic and relapsing addictive trait that harms public health. Among the many identified genetic variants of nicotine dependence, the variants in the CHRNA5\/A3\/B4 gene cluster on chromosome 15 that encode the \u03b15, \u03b13, and \u03b24 subunits have recently received a lot of attention. Importantly, variants in this gene cluster have been associated with nicotine addiction. Among the many significant variants in this cluster, the polymorphism SNP rs16969968 seems to be the most interesting factor in nicotine addiction. This polymorphism causes an amino acid change from aspartate to asparagine at position 398 of the \u03b15 nicotinic receptor protein sequence. Our study aimed to analyze three polymorphic variants: the rs16969968 located in the CHRNA5 gene, the rs578776 and rs1051730 located in the CHRNA3 gene in nicotine-addicted subjects, and in controls. Our study encompasses an association analysis of genotypes and haplotypes. A group of 401 volunteers was recruited for the study and divided into two groups: the study group consisted of addicted smokers and a control group of 200 unrelated non-smokers who were not dependent on any substance and healthy. A statistically significant difference was observed in the frequency of genotypes of the rs1051730 polymorphism of the CHRNA3 gene (\u03c72 = 6.704 p = 0.035). The T\/T genotype was statistically significantly more frequent in the group of nicotine-dependent subjects. The haplotypes rs16969968, rs578776, and rs1051730 were distinguished, of which the G-T-T and G-C-T haplotypes were present only in the study group. With differences in frequencies, statistical significance was noted\u2014for the G-T-T haplotype p = 0.01284 and the G-C-T haplotype p = 0.00775. The research stated that novel haplotypes G-T-T and G-C-T, though with very low-frequency variants in CHRNA3, were associated with nicotine addiction.<\/jats:p>","DOI":"10.3390\/ijerph191710478","type":"journal-article","created":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T21:05:12Z","timestamp":1661288712000},"page":"10478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Association of Polymorphism CHRNA5 and CHRNA3 Gene in People Addicted to Nicotine"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4254-5466","authenticated-orcid":false,"given":"Krzysztof","family":"Chmielowiec","sequence":"first","affiliation":[{"name":"Department of Hygiene and Epidemiology, Collegium Medicum, University of Zielona G\u00f3ra, 28 Zyty St., 65-046 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3285-5313","authenticated-orcid":false,"given":"Jolanta","family":"Chmielowiec","sequence":"additional","affiliation":[{"name":"Department of Hygiene and Epidemiology, Collegium Medicum, University of Zielona G\u00f3ra, 28 Zyty St., 65-046 Zielona Gora, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6564-2141","authenticated-orcid":false,"given":"Aleksandra","family":"Stro\u0144ska-Pluta","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3900-4901","authenticated-orcid":false,"given":"Grzegorz","family":"Trybek","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pomeranian Medical University in Szczecin, 72 Powstanc\u00f3w Wlkp. St., 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"\u015amiarowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Therapeutic Drug Monitoring, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-1429","authenticated-orcid":false,"given":"Aleksandra","family":"Suchanecka","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Private Dental Practice, 9 Bahnhofstrasse, 3940 Steg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7727-6388","authenticated-orcid":false,"given":"Aleksandra","family":"Jaro\u0144","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pomeranian Medical University in Szczecin, 72 Powstanc\u00f3w Wlkp. St., 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2633-520X","authenticated-orcid":false,"given":"Anna","family":"Grzywacz","sequence":"additional","affiliation":[{"name":"Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,23]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2013). World Health Organization WHO Report on the Global Tobacco Epidemic, 2013 Enforcing Bans on Tobacco Advertising, Promotion and Sponsors, World Health Organization. Hip Fresh and Alive Mpower Includes a Special Section on Five Years of Progress."},{"key":"ref_2","first-page":"132","article-title":"The Tobacco Atlas, Fourth Edition","volume":"176","author":"Eriksen","year":"2012","journal-title":"Am. J. 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