{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T18:07:01Z","timestamp":1783966021183,"version":"3.55.0"},"reference-count":61,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2021YFD1800700"],"award-info":[{"award-number":["2021YFD1800700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["CAAS-FRI-06"],"award-info":[{"award-number":["CAAS-FRI-06"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["2021YFD1800700"],"award-info":[{"award-number":["2021YFD1800700"]}]},{"name":"Innovation Project of Chinese Academy of Agricultural Sciences","award":["CAAS-FRI-06"],"award-info":[{"award-number":["CAAS-FRI-06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Dairy manure is a nutrition source for cropland soils and also simultaneously serves as a contamination source of antibiotic resistance genes (ARGs). In this study, five classes of antibiotics including aminoglycosides, beta-lactams, macrolides, sulfonamides, and tetracyclines, were spiked in dairy manure and incubated with soil for 60 days. The high throughput qPCR and 16S rRNA amplicon sequencing were used to detect temporal shifts of the soil antibiotic resistomes and bacterial community. Results indicated dairy manure application increased the ARG abundance by 0.5\u20133.7 times and subtype numbers by 2.7\u20133.7 times and changed the microbial community structure in soils. These effects were limited to the early incubation stage. Selection pressure was observed after the addition of sulfonamides. Bacterial communities played an important role in the shifts of ARG profiles and accounted for 44.9% of the resistome variation. The incubation period, but not the different antibiotic treatments, has a strong impact on the bacteria community. Firmicutes and Bacteroidetes were the dominant bacterial hosts for individual ARGs. This study advanced our understanding of the effect of dairy manure and antibiotics on the antibiotic resistome in soils and provided a reference for controlling ARG dissemination from dairy farms to the environment.<\/jats:p>","DOI":"10.3390\/ijerph191710804","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T21:25:18Z","timestamp":1661894718000},"page":"10804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Shifts of Antibiotic Resistomes in Soil Following Amendments of Antibiotics-Contained Dairy Manure"],"prefix":"10.3390","volume":"19","author":[{"given":"Jijun","family":"Kang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9779-7321","authenticated-orcid":false,"given":"Yiming","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojie","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3346-7689","authenticated-orcid":false,"given":"Fei","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenguang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Veterinary Pharmaceutic Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiubo","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.envint.2017.07.003","article-title":"Environmental and human health challenges of industrial livestock and poultry farming in China and their mitigation","volume":"107","author":"Hu","year":"2017","journal-title":"Environ. Int."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Tiseo, K., Huber, L., Gilbert, M., Robinson, T.P., and Van Boeckel, T.P. (2020). Global trends in antimicrobial use in food animals from 2017 to 2030. Antibiotics, 9.","DOI":"10.3390\/antibiotics9120918"},{"key":"ref_3","first-page":"20162233","article-title":"Exposure to dairy manure leads to greater antibiotic resistance and increased mass-specific respiration in soil microbial communities","volume":"284","author":"Wepking","year":"2017","journal-title":"Proc. Biol. Sci."},{"key":"ref_4","unstructured":"China, M.O. (2018, April 20). Action of Reduction of Antimicrobial Agents Used in Veterinary Practice (2018\u20132021), Available online: http:\/\/www.moa.gov.cn\/govpublic\/SYJ\/201804\/t20180420_6140711.htm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jhin.2015.11.010","article-title":"Tigecycline as last resort in severe refractory Clostridium difficile infection: A case report","volume":"92","author":"Knafl","year":"2016","journal-title":"J. Hosp. Infect."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"139306","DOI":"10.1016\/j.scitotenv.2020.139306","article-title":"Exploring tet(X)-bearing tigecycline-resistant bacteria of swine farming environments","volume":"733","author":"Li","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7465","DOI":"10.1111\/1462-2920.15632","article-title":"The poultry pathogen Riemerella anatipestifer appears as a reservoir for Tet(X) tigecycline resistance","volume":"23","author":"Umar","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s13073-020-00807-5","article-title":"Genetic diversity and characteristics of high-level tigecycline resistance Tet(X) in Acinetobacter species","volume":"12","author":"Chen","year":"2020","journal-title":"Genome Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100882","DOI":"10.1016\/j.cofs.2022.100882","article-title":"Role of Horizontal Gene Transfer in the dissemination of antimicrobial resistance in food animal production","volume":"47","author":"Vinayamohan","year":"2022","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"114930","DOI":"10.1016\/j.envpol.2020.114930","article-title":"Tracing veterinary antibiotics in the subsurface\u2014A long-term field experiment with spiked manure","volume":"265","author":"Mehrtens","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"110567","DOI":"10.1016\/j.ecoenv.2020.110567","article-title":"Family livestock waste: An ignored pollutant resource of antibiotic resistance genes","volume":"197","author":"Gu","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104912","DOI":"10.1016\/j.envint.2019.104912","article-title":"Transfer of antibiotic resistance from manure-amended soils to vegetable microbiomes","volume":"130","author":"Zhang","year":"2019","journal-title":"Environ. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e2019GH000213","DOI":"10.1029\/2019GH000213","article-title":"A review of potential public health impacts associated with the global dairy sector","volume":"4","author":"Grout","year":"2020","journal-title":"Geohealth"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"149916","DOI":"10.1016\/j.scitotenv.2021.149916","article-title":"Transformation products of pharmaceuticals in the environment: Their fate, (eco)toxicity and bioaccumulation potential","volume":"802","author":"Maculewicz","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.vetmic.2017.12.022","article-title":"Shift of uterine microbiota associated with antibiotic treatment and cure of metritis in dairy cows","volume":"214","author":"Jeon","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.3168\/jds.2019-16778","article-title":"Invited review: Fate of antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes in US dairy manure management systems","volume":"103","author":"Oliver","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1292\/jvms.19-0035","article-title":"Influence of oxytetracycline susceptibility as a first-line antibiotic on the clinical outcome in dairy cattle with acute Escherichia coli mastitis","volume":"81","author":"Shinozuka","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s40168-019-0696-4","article-title":"Antibiotic treatment in feedlot cattle: A longitudinal study of the effect of oxytetracycline and tulathromycin on the fecal and nasopharyngeal microbiota","volume":"7","author":"Holman","year":"2019","journal-title":"Microbiome"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.envint.2019.04.043","article-title":"Effect of antibiotic use and composting on antibiotic resistance gene abundance and resistome risks of soils receiving manure-derived amendments","volume":"128","author":"Chen","year":"2019","journal-title":"Environ. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"108252","DOI":"10.1016\/j.soilbio.2021.108252","article-title":"Application of manure from cattle administered antibiotics has sustained multi-year impacts on soil resistome and microbial community structure","volume":"157","author":"Shawver","year":"2021","journal-title":"Soil Biol. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1128\/AEM.02577-10","article-title":"Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine","volume":"77","author":"Heuer","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.ecoenv.2017.08.061","article-title":"Application of manure containing tetracyclines slowed down the dissipation of tet resistance genes and caused changes in the composition of soil bacteria","volume":"147","author":"Xiong","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"120729","DOI":"10.1016\/j.jhazmat.2019.06.006","article-title":"Interaction among soil physicochemical properties, bacterial community structure, and arsenic contamination: Clay-induced change in long-term arsenic contaminated soils","volume":"378","author":"Jeong","year":"2019","journal-title":"J. Hazard Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/j.envpol.2019.02.093","article-title":"Antibiotic resistance genes and bacterial communities in cornfield and pasture soils receiving swine and dairy manures","volume":"248","author":"Chen","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108215","DOI":"10.1016\/j.jnutbio.2019.07.006","article-title":"Interaction between high-fat diet and ethanol intake leads to changes on the fecal microbiome","volume":"72","author":"Pedersen","year":"2019","journal-title":"J. Nutr. Biochem."},{"key":"ref_26","unstructured":"Bushnell, B. (2017, December 08). BBmap Files. Available online: https:\/\/sourceforge.net\/projects\/bbmap\/files\/."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Magoc","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e2584","DOI":"10.7717\/peerj.2584","article-title":"VSEARCH: A versatile open source tool for metagenomics","volume":"4","author":"Rognes","year":"2016","journal-title":"PeerJ"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"D643","DOI":"10.1093\/nar\/gkt1209","article-title":"The SILVA and \u201cAll-species Living Tree Project (LTP)\u201d taxonomic frameworks","volume":"42","author":"Yilmaz","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1002\/imt2.5","article-title":"ImageGP: An easy-to-use data visualization web server for scientific researchers","volume":"1","author":"Chen","year":"2022","journal-title":"iMeta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"129163","DOI":"10.1016\/j.jhazmat.2022.129163","article-title":"Long-term manure inputs induce a deep selection on agroecosystem soil antibiotic resistome","volume":"436","author":"Liu","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_34","first-page":"fux053","article-title":"Environmental factors influencing the development and spread of antibiotic resistance","volume":"42","author":"Kristiansson","year":"2018","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"123816","DOI":"10.1016\/j.jhazmat.2020.123816","article-title":"Profiles of antibiotic resistome and microbial community in groundwater of CKDu prevalence zones in Sri Lanka","volume":"403","author":"Cooray","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"142217","DOI":"10.1016\/j.scitotenv.2020.142217","article-title":"Occurrence of emerging sulfonamide resistance (sul1 and sul2) associated with mobile integrons-integrase (intI1 and intI2) in riverine systems","volume":"751","author":"Chaturvedi","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.watres.2018.03.025","article-title":"Emerging contaminants and nutrients synergistically affect the spread of class 1 integron-integrase (intI1) and sul1 genes within stable streambed bacterial communities","volume":"138","author":"Subirats","year":"2018","journal-title":"Water Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.jes.2020.07.016","article-title":"Dairy manure as a potential source of crop nutrients and environmental contaminants","volume":"100","author":"Hill","year":"2021","journal-title":"J. Environ. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"113555","DOI":"10.1016\/j.ecoenv.2022.113555","article-title":"Manure application: A trigger for vertical accumulation of antibiotic resistance genes in cropland soils","volume":"237","author":"Mu","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e01017","DOI":"10.1128\/mBio.01017-13","article-title":"Diverse antibiotic resistance genes in dairy cow manure","volume":"5","author":"Wichmann","year":"2014","journal-title":"MBio"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1016\/j.envpol.2017.09.074","article-title":"Temporal succession of soil antibiotic resistance genes following application of swine, cattle and poultry manures spiked with or without antibiotics","volume":"231","author":"Zhang","year":"2017","journal-title":"Environ. Pollut."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.jes.2021.04.019","article-title":"Profiling the antibiotic resistome in soils between pristine and human-affected sites on the Tibetan Plateau","volume":"111","author":"Wang","year":"2022","journal-title":"J. Environ. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.watres.2018.02.003","article-title":"Complex pollution of antibiotic resistance genes due to beta-lactam and aminoglycoside use in aquaculture farming","volume":"134","author":"Chen","year":"2018","journal-title":"Water Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.1021\/acs.est.6b05342","article-title":"Comparison of metals and tetracycline as selective agents for development of tetracycline resistant bacterial communities in agricultural soil","volume":"51","author":"Song","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.soilbio.2014.11.018","article-title":"Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure","volume":"81","author":"Bhumibhamon","year":"2015","journal-title":"Soil. Biol. Biochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"145399","DOI":"10.1016\/j.scitotenv.2021.145399","article-title":"Targeted metagenomics reveals inferior resilience of farm soil resistome compared to soil microbiome after manure application","volume":"770","author":"Macedo","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.chemosphere.2018.09.083","article-title":"Sorption behaviors of antimicrobial and antiparasitic veterinary drugs on subtropical soils","volume":"214","author":"Rath","year":"2019","journal-title":"Chemosphere"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"153415","DOI":"10.1016\/j.scitotenv.2022.153415","article-title":"Understanding flow patterns from the field\u2014Controlled laboratory experiments on the transport behavior of veterinary antibiotics in the presence of liquid manure","volume":"821","author":"Mehrtens","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"150023","DOI":"10.1016\/j.scitotenv.2021.150023","article-title":"Fate of sulfamethoxazole in compost, manure and soil amended with previously stored organic wastes","volume":"803","author":"Dellouh","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3854","DOI":"10.1021\/acs.est.7b06026","article-title":"Bacteria that make a meal of sulfonamide antibiotics: Blind spots and emerging opportunities","volume":"52","author":"Deng","year":"2018","journal-title":"Environ Sci. Technol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"132734","DOI":"10.1016\/j.chemosphere.2021.132734","article-title":"Fate of bacterial community, antibiotic resistance genes and gentamicin residues in soil after three-year amendment using gentamicin fermentation waste","volume":"291","author":"Liu","year":"2022","journal-title":"Chemosphere"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"22185","DOI":"10.1038\/s41598-021-99001-9","article-title":"Soil bacterial community as impacted by addition of rice straw and biochar","volume":"11","author":"Tang","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"104466","DOI":"10.1016\/j.apsoil.2022.104466","article-title":"Cattle manure application triggers short-term dominance of Acinetobacter in soil microbial communities","volume":"176","year":"2022","journal-title":"Appl. Soil Ecol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Zaheer, R., Lakin, S.M., Polo, R.O., Cook, S.R., Larney, F.J., Morley, P.S., Booker, C.W., Hannon, S.J., Van Domselaar, G., and Read, R.R. (2019). Comparative diversity of microbiomes and Resistomes in beef feedlots, downstream environments and urban sewage influent. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1548-x"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"123399","DOI":"10.1016\/j.jhazmat.2020.123399","article-title":"Dynamics of the antibiotic resistome in agricultural soils amended with different sources of animal manures over three consecutive years","volume":"401","author":"Liu","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.jhazmat.2018.06.052","article-title":"Contributions of the microbial community and environmental variables to antibiotic resistance genes during co-composting with swine manure and cotton stalks","volume":"358","author":"Zhang","year":"2018","journal-title":"J. Hazard. Mater."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.envint.2016.03.026","article-title":"Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil","volume":"92","author":"Chen","year":"2016","journal-title":"Environ. Int."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"152157","DOI":"10.1016\/j.scitotenv.2021.152157","article-title":"Swine manure facilitates the spread of antibiotic resistome including tigecycline-resistant tet(X) variants to farm workers and receiving environment","volume":"808","author":"Tong","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"155039","DOI":"10.1016\/j.scitotenv.2022.155039","article-title":"Effects of composting on the fate of doxycycline, microbial community, and antibiotic resistance genes in swine manure and broiler manure","volume":"832","author":"Xu","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"141113","DOI":"10.1016\/j.scitotenv.2020.141113","article-title":"Composting of chicken litter from commercial broiler farms reduces the abundance of viable enteric bacteria, Firmicutes, and selected antibiotic resistance genes","volume":"746","author":"Subirats","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"148372","DOI":"10.1016\/j.scitotenv.2021.148372","article-title":"Key factors driving the fate of antibiotic resistance genes and controlling strategies during aerobic composting of animal manure: A review","volume":"791","author":"Liu","year":"2021","journal-title":"Sci. Total Environ."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/17\/10804\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:17Z","timestamp":1760142017000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/19\/17\/10804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":61,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["ijerph191710804"],"URL":"https:\/\/doi.org\/10.3390\/ijerph191710804","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,30]]}}}