{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T10:41:17Z","timestamp":1779014477916,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:00:00Z","timestamp":1663113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Agricultura y Desarrollo Rural","award":["2015"],"award-info":[{"award-number":["2015"]}]},{"name":"Ministerio de Agricultura y Desarrollo Rural","award":["2015-2016"],"award-info":[{"award-number":["2015-2016"]}]},{"name":"Ministerio de Agricultura y Desarrollo Rural","award":["2018\u20132019"],"award-info":[{"award-number":["2018\u20132019"]}]},{"DOI":"10.13039\/501100002906","name":"Porkcolombia","doi-asserted-by":"publisher","award":["2015"],"award-info":[{"award-number":["2015"]}],"id":[{"id":"10.13039\/501100002906","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002906","name":"Porkcolombia","doi-asserted-by":"publisher","award":["2015-2016"],"award-info":[{"award-number":["2015-2016"]}],"id":[{"id":"10.13039\/501100002906","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002906","name":"Porkcolombia","doi-asserted-by":"publisher","award":["2018\u20132019"],"award-info":[{"award-number":["2018\u20132019"]}],"id":[{"id":"10.13039\/501100002906","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad de Antioquia","award":["2015"],"award-info":[{"award-number":["2015"]}]},{"name":"Universidad de Antioquia","award":["2015-2016"],"award-info":[{"award-number":["2015-2016"]}]},{"name":"Universidad de Antioquia","award":["2018\u20132019"],"award-info":[{"award-number":["2018\u20132019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Gram-negative spirochete Leptospira spp. causes leptospirosis. Leptospirosis is still a neglected disease, even though it can cause potentially fatal infections in a variety of species including humans. The purpose of this study was to determine the seroprevalence of leptospirosis in pig farm captured rodents and characterize the isolated samples. Rats were captured, sampled, and euthanized in the vicinity of pig farms to obtain serum for microagglutination tests (MAT) and kidney tissues for PCR amplification of the 16S rRNA and LipL32 genes. A fraction of the 16S rRNA PCR product was sequenced and phylogenetically analyzed. The results showed a Leptospira seroprevalence of 13.8% (77\/555) among the 555 captured rats. PCR positivity for Leptospira spp. reached 31.2% (156\/500), and the positivity for pathogenic Leptospira spp. was 4% (22\/500). Phylogenetic analysis matched eight samples with L. interrogans serovar icterohaemorrhagiae and two with L. interrogans serovar pyrogenes. Two sequences were located within the pathogenic Leptospira clade but did not match with any specific strain. The seroprevalence found in the rats around swine farms indicates a potential risk of transmission to the pigs. The identification of pathogenic Leptospira outlines the importance of more research as well as updating the current strategies for the diagnosis, control, and prevention of porcine leptospirosis in Colombia.<\/jats:p>","DOI":"10.3390\/ijerph191811539","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T20:50:45Z","timestamp":1663188645000},"page":"11539","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Seroprevalence and Molecular Characterization of Leptospira spp. in Rats Captured near Pig Farms in Colombia"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4910-1705","authenticated-orcid":false,"given":"Sara","family":"L\u00f3pez-Osorio","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n CIBAV, Facultad de Ciencias Agrarias, Universidad de Antioquia-UdeA, Medell\u00edn 050034, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego A.","family":"Molano","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n CIBAV, Facultad de Ciencias Agrarias, Universidad de Antioquia-UdeA, Medell\u00edn 050034, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson","family":"L\u00f3pez-Arias","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n CIBAV, Facultad de Ciencias Agrarias, Universidad de Antioquia-UdeA, Medell\u00edn 050034, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-979X","authenticated-orcid":false,"given":"N\u00e9lida","family":"Rodr\u00edguez-Osorio","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00f3mica y Bioinform\u00e1tica, Departamento de Ciencias Biol\u00f3gicas, Centro Universitario Regional Litoral Norte, Universidad de la Rep\u00fablica, Rivera 1350, Salto 50000, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corina","family":"Zambrano","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n Porkcolombia-FNP, Ceniporcino, Bogot\u00e1 111311, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2750-0721","authenticated-orcid":false,"given":"Jenny J.","family":"Chaparro-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n CIBAV, Facultad de Ciencias Agrarias, Universidad de Antioquia-UdeA, Medell\u00edn 050034, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,14]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (2021, June 10). Leptospirosis, Available online: https:\/\/www.cdc.gov\/leptospirosis\/health_care_workers\/index.html#:~:text=Although%20incidence%20in%20the%20United,including%20an%20about%2059%2C000%20deaths."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.biologicals.2013.06.010","article-title":"Leptospirosis: Public health perspectives","volume":"41","author":"Guerra","year":"2013","journal-title":"Biologicals"},{"key":"ref_3","first-page":"204","article-title":"Towards a \u201cOne Health\u201d strategy against Leptospirosis","volume":"2","author":"Jancloes","year":"2014","journal-title":"Planet@ Risk"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.ijid.2011.05.017","article-title":"Strikes, flooding, rats, and leptospirosis in Marseille, France","volume":"15","author":"Socolovschi","year":"2011","journal-title":"Int. J. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1099\/jmm.0.011528-0","article-title":"Human leptospirosis cases and the prevalence of rats harbouring Leptospira interrogans in urban areas of Tokyo, Japan","volume":"58","author":"Koizumi","year":"2009","journal-title":"J. Med. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.cliser.2018.09.001","article-title":"Predictability of seasonal precipitation across major crop growing areas in Colombia","volume":"12","author":"Esquivel","year":"2018","journal-title":"Clim. Serv."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Lizumi, T., Luo, J.J., Challinor, A., Sakurai, G., Yokozawa, M., Sauma, H., Brown, M.E., and Yamagata, T. (2014). Impacts of El Ni\u00f1o Southern Oscillation on the global yields of major crops. Nat. Commun., 5.","DOI":"10.1038\/ncomms4712"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"204","DOI":"10.15446\/rsap.v19n2.54235","article-title":"Prevalence of Leptospirosis in Colombia: Systematic literature review","volume":"19","author":"Salas","year":"2017","journal-title":"Rev. Salud P\u00fablica"},{"key":"ref_9","unstructured":"Instituto Geogr\u00e1fico Agust\u00edn Codazzi (2012). Atlas B\u00e1sico de Colombia."},{"key":"ref_10","unstructured":"DANE (2019, October 18). Encuesta de Sacrificio de Ganado-ESAG, Available online: https:\/\/www.dane.gov.co\/index.php\/estadisticas-por-tema\/agropecuario\/encuesta-de-sacrificio-de-ganado."},{"key":"ref_11","first-page":"99","article-title":"Animal Leptospirosis","volume":"387","author":"Ellis","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_12","unstructured":"Divers, P.R. (2021, June 10). Leptospirosis in Swine. Available online: https:\/\/www.merckvetmanual.com\/generalized-conditions\/leptospirosis\/leptospirosis-in-swine."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1590\/S0103-84782004000300029","article-title":"Fatores de risco associados a soropositividade para leptospirose em matrizae su\u00ednas","volume":"34","author":"Delbem","year":"2004","journal-title":"Ci\u00eancia Rural."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bierque, E., Thibeaux, R., Girault, D., Soup\u00e9-Gilbert, M., and Goarant, C. (2020). A systemic review of Leptospira in water and soil environments. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0227055"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Boey, K., Shiokawa, K., and Rajeev, S. (2019). Leptospira infection in rats: A literature review of global prevalence and distribution. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007499"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Pellizzaro, M., Martins, C.M., Yamakawa, A.C., Ferraz, D.D.C., Morikawa, V.M., Ferreira, F., Pires do Santos, A., Welker, A., and Langoni, H. (2019). Molecular detection of Leptospira spp. in rats as early spatial predictor for human disease in an endemic urban area. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0216830"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1007\/s11252-020-00957-9","article-title":"Prevalence and risk factors of Leptospira infection in urban brown rats (Rattus norvegicus)","volume":"23","author":"Smith","year":"2020","journal-title":"Urban Ecosyst."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Benavidez, K.M., Guerra, T., Torres, M., Rodriguez, D., Veech, J.A., Hahn, D., Miller, R.J., Soltero, F.V., P\u00e9rez-Ramirez, A., and P\u00e9rez de Le\u00f3n, A. (2019). The prevalence of Leptospira among invasive small mammals on Puerto Rican cattle farms. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007236"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.actatropica.2018.08.020","article-title":"Molecular characterization of pathogenic Leptospira sp. in small mammals captured from the human leptospirosis suspected areas of Selangor state, Malaysia","volume":"188","author":"Azhari","year":"2018","journal-title":"Acta Trop."},{"key":"ref_20","unstructured":"Elias, D.J. (1984). Rodents as Pests of Stored Products: Control and Management [Online] Tecnolog\u00eda Postcosecha #3, Oficina Regional de la FAO para Am\u00e9rica Latina y el Caribe. Available online: https:\/\/nwrc.contentdm.oclc.org\/digital\/collection\/NWRCPubs1\/id\/29443."},{"key":"ref_21","unstructured":"Brown, R.Z. (1960). Biological Factors in Domestic Rodent Control."},{"key":"ref_22","unstructured":"World Health Organization (2021, July 21). Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Available online: https:\/\/www.who.int\/publications\/i\/item\/human-leptospirosis-guidance-for-diagnosis-surveillance-and-control."},{"key":"ref_23","first-page":"9","article-title":"Rapid diagnosis of leptospirosis by multiplex PCR","volume":"19","author":"Ahmed","year":"2012","journal-title":"Malays. J. Med. Sci."},{"key":"ref_24","first-page":"3510","article-title":"Species-Specific Identification of Leptospiraceae by 16S rRNA Gene Sequencing","volume":"44","author":"Morey","year":"2006","journal-title":"J. Microbiol."},{"key":"ref_25","unstructured":"IDEAM, Institute of Hydrology, Meteorology and Environmental Studies (2022, July 09). Climatological Atlas of Colombia, Available online: http:\/\/atlas.ideam.gov.co\/visorAtlasClimatologico.html."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mahlaba, T.A.M., Monadjem, A., McCleery, R., and Belmain, S.R. (2017). Domestic cats and dogs create a landscape of fear for pest rodents around rural homesteads. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0171593"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1084\/jem.26.3.341","article-title":"The rat as a carrier of spirochaeta icterohaemorrhagiae, the causative agent of Weil\u2019s disease (Spirochaetosis icterohemorrhagica)","volume":"26","author":"Ido","year":"1917","journal-title":"J. Exp. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3109\/23744235.2015.1092579","article-title":"Is it too cold for Leptospira innterrogans transmission on the Faroese Islands?","volume":"48","author":"Jensen","year":"2015","journal-title":"Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Biscornet, L., Revillion, C., Jego, S., Lagadec, E., Gomard, Y., Minter, G.L., Rocamora, G., Guernier-Camber, V., Melade, J., and Dellagi, K. (2021). Predicting the presence of leptospires in rodents from enviornmental indicators opens up opportunities for viornmental monitoring of human leptospirosis. Int. J. Remote Sens., 13.","DOI":"10.3390\/rs13020325"},{"key":"ref_30","first-page":"115","article-title":"Leptospirosis in wild rodents in the rural area of Valdivia. Detection of Leptospira interrogans by immunofluorescence and immunoperoxidase","volume":"1","author":"Zamora","year":"1995","journal-title":"Arch. Med. Vet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"150","DOI":"10.30972\/vet.1921897","article-title":"Prevalence of leptospirosis in synathropic rodents of the city of Corrientes, Argentina. Period of May 2005\u2013June 2008","volume":"19","author":"Marder","year":"2008","journal-title":"Rev Vet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and Leptospirosis","volume":"140","author":"Adler","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_33","first-page":"69","article-title":"Rodents as Leptospira reservoirs at swine farms of the central coffee grower area of Colombia","volume":"34","author":"Betancurth","year":"2002","journal-title":"Arch. Med. Vet."},{"key":"ref_34","unstructured":"Urrea, V., Ochoa, A., and Mesa, O. (2016, January 26\u201330). Estacionalidad de la lluvia en Colombia. Proceedings of the XXVII Congreso Latinoamericano de Hidr\u00e1ulic, Lima, Per\u00fa."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Vincent, A.T., Schiettekatte, O., Goarant, C., Kumari Neela, V., Bernet, E., Thibeaux, R., Ismail, N., Khairul Nizam Mohd Khalid, M., Amran, F., and Masuzawa, T. (2019). Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007270"},{"key":"ref_36","first-page":"179","article-title":"Molecular serovar characterization of Leptospira isolates from animals and water in Colombia","volume":"33","author":"Thiry","year":"2013","journal-title":"Biom\u00e9dica"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1179\/136485911X12899838683205","article-title":"Maximizing the chances of detecting pathogenic leptospires in mammals: The evaluation of field samples and a multi-sample-per-mammal, multi-test approach","volume":"105","author":"Tulsiani","year":"2011","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1590\/0074-02760160245","article-title":"Genetic diversity of Leptospira in northwestern Colombia: First report of Leptospira santarosai as a recognised leptospirosis agent","volume":"111","author":"Lopez","year":"2016","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Santos, L.A., Adhikarla, H., Yan, X., Wang, Z., Fouts, D.E., Vinetz, J.M., Alcantara, L.J.C., Hartskeerl, R.A., Goris, M.G.A., and Picardeau, M. (2018). Genomic Comparison Among Global Isolates of L. interrogans Serovats Copenhagen and Icterohaemorrhagiae identified Natural Genetic Variation Caused by an Indel. Front. Cell. Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00193"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"906","DOI":"10.4269\/ajtmh.2009.09-0195","article-title":"Prevalence of Leptospira spp. in Urban Rodents from a Groceries Trade Center of Medellin, Colombia","volume":"81","author":"Quiroz","year":"2009","journal-title":"Am. J. Trop. Med. 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