{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:57:05Z","timestamp":1783461425286,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Inflammation is a normal response in defense to agents that may cause damage to the human body. When inflammation becomes chronic, reactive oxygen species (ROS) are produced; which could lead to diseases such as cancer. The aim was to assess liposomes\u2019 antioxidant and anti-inflammatory capacity loaded with amaranth unsaponifiable matter and soybean lunasin (UM + LunLip) in an in vitro model using fibroblasts and macrophages. To evaluate ROS production, fibroblasts CHON-002 ABAP were added to promote ROS production; and the cells were treated with UM + LunLip. For inflammation markers production, lipopolysaccharides (LPS)-stimulated RAW 264.7 and peritoneal macrophages were treated with empty liposomes (EmLip), liposomes loaded with unsaponifiable matter (UMLip), liposomes loaded with lunasin (LunLip), and UM + LunLip. ROS production was significantly decreased by 77% (p &lt; 0.05) when fibroblasts were treated with UM + LunLip at 2 mg lunasin\/mL compared with the control treated with ABAP. Treatment with UMLip was the most effective in reducing tumor necrosis factor-\u03b1 (71\u201390%) and interleukin-6 (43\u201355%, p &lt; 0.001). Both liposomes containing unsaponifiable matter (UMLip and UM + LunLip) were more effective than EmLip or LunLip. In conclusion, amaranth unsaponifiable matter-loaded liposomes are effective in decreasing pro-inflammatory cytokine production.<\/jats:p>","DOI":"10.3390\/ijerph191811678","type":"journal-article","created":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T23:39:22Z","timestamp":1663544362000},"page":"11678","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages"],"prefix":"10.3390","volume":"19","author":[{"given":"Gloria","family":"D\u00e1vila-Ortiz","sequence":"first","affiliation":[{"name":"Unidad Profesional Adolfo L\u00f3pez Mateos, Departamento de Ingenier\u00eda Bioqu\u00edmica, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Campus Zacatenco, Madero, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-0216","authenticated-orcid":false,"given":"Erick Damian","family":"Casta\u00f1eda-Reyes","sequence":"additional","affiliation":[{"name":"Unidad Profesional Adolfo L\u00f3pez Mateos, Departamento de Ingenier\u00eda Bioqu\u00edmica, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Campus Zacatenco, Madero, Ciudad de M\u00e9xico 07738, Mexico"},{"name":"Department of Food Science and Human Nutrition, University of Illinois, Urbana-Champaign, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Ignacio","family":"Ju\u00e1rez-Palomo","sequence":"additional","affiliation":[{"name":"Unidad Profesional Adolfo L\u00f3pez Mateos, Departamento de Ingenier\u00eda Bioqu\u00edmica, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Campus Zacatenco, Madero, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0441-9891","authenticated-orcid":false,"given":"Mar\u00eda de Jes\u00fas","family":"Perea-Flores","sequence":"additional","affiliation":[{"name":"Unidad Profesional Adolfo L\u00f3pez Mateos, Centro de Nanociencias y Micro y Nanotecnolog\u00edas, Instituto Polit\u00e9cnico Nacional (IPN), Av. Luis Enrique Erro s\/n, Zacatenco, Alcald\u00eda Gustavo A. Madero, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1069-5558","authenticated-orcid":false,"given":"Ricardo","family":"P\u00e9rez-Past\u00e9n-Borja","sequence":"additional","affiliation":[{"name":"Unidad Profesional Adolfo L\u00f3pez Mateos, Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Campus Zacatenco, Madero, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0132-3149","authenticated-orcid":false,"given":"Yazm\u00edn Karina","family":"M\u00e1rquez-Flores","sequence":"additional","affiliation":[{"name":"Unidad Profesional Adolfo L\u00f3pez Mateos, Departamento de Farmacia, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Campus Zacatenco, Madero, Ciudad de M\u00e9xico 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7426-9035","authenticated-orcid":false,"given":"Elvira","family":"Gonz\u00e1lez de Mej\u00eda","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, University of Illinois, Urbana-Champaign, IL 61801, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1093\/intimm\/dxy054","article-title":"Macrophages in inflammation, repair and regeneration","volume":"30","author":"Oishi","year":"2018","journal-title":"Int. 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