{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:59:15Z","timestamp":1787497155900,"version":"build-2736575974"},"reference-count":36,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006114","name":"State Ministry of Science and Cultural Affairs of Saxony, Germany","doi-asserted-by":"publisher","award":["100535976"],"award-info":[{"award-number":["100535976"]}],"id":[{"id":"10.13039\/501100006114","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Dependent on the excretion pattern, wastewater monitoring of viruses can be a valuable approach to characterizing their circulation in the human population. Using polyethylene glycol precipitation and reverse transcription-quantitative PCR, the occurrence of RNA of SARS-CoV-2 and influenza viruses A\/B in the raw wastewater of two treatment plants in Germany between January and May 2022 was investigated. Due to the relatively high incidence in both exposal areas (plant 1 and plant 2), SARS-CoV-2-specific RNA was determined in all 273 composite samples analyzed (concentration of E gene: 1.3 \u00d7 104 to 3.2 \u00d7 106 gc\/L). Despite a nation-wide low number of confirmed infections, influenza virus A was demonstrated in 5.2% (concentration: 9.8 \u00d7 102 to 8.4 \u00d7 104 gc\/L; plant 1) and in 41.6% (3.6 \u00d7 103 to 3.0 \u00d7 105 gc\/L; plant 2) of samples. Influenza virus B was detected in 36.0% (7.2 \u00d7 102 to 8.5 \u00d7 106 gc\/L; plant 1) and 57.7% (9.6 \u00d7 103 to 2.1 \u00d7 107 gc\/L; plant 2) of wastewater samples. The results of the study demonstrate the frequent detection of two primary respiratory viruses in wastewater and offer the possibility to track the epidemiology of influenza by wastewater-based monitoring.<\/jats:p>","DOI":"10.3390\/ijerph192013374","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T03:43:58Z","timestamp":1665978238000},"page":"13374","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Simultaneous Detection of SARS-CoV-2 and Influenza Virus in Wastewater of Two Cities in Southeastern Germany, January to May 2022"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6351-8817","authenticated-orcid":false,"given":"Roger","family":"Dumke","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology und Virology, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0265-8490","authenticated-orcid":false,"given":"Michael","family":"Geissler","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology und Virology, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annett","family":"Skupin","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology und Virology, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4963-7523","authenticated-orcid":false,"given":"Bj\u00f6rn","family":"Helm","sequence":"additional","affiliation":[{"name":"Institute of Urban and Industrial Water Management, Technische Universit\u00e4t Dresden, 01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Mayer","sequence":"additional","affiliation":[{"name":"Institute of Urban and Industrial Water Management, Technische Universit\u00e4t Dresden, 01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Schubert","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Faculty of Medicine Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"},{"name":"Institute of Hydrobiology, Technische Universit\u00e4t Dresden, 01217 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Oertel","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Faculty of Medicine Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0845-6793","authenticated-orcid":false,"given":"Bertold","family":"Renner","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Faculty of Medicine Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1648-6618","authenticated-orcid":false,"given":"Alexander H.","family":"Dalpke","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology und Virology, University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden, 01307 Dresden, Germany"},{"name":"Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Corpuz, M.V.A., Buonerba, A., Vigliotta, G., Zarra, T., Ballesteros, F., Campiglia, P., Belgiorno, V., Korshin, G., and Naddeo, V. 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