{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:45:58Z","timestamp":1787028358589,"version":"build-2736575974"},"reference-count":18,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T00:00:00Z","timestamp":1670025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006506","name":"Brazilian Ministry of Health","doi-asserted-by":"publisher","award":["54\/2017"],"award-info":[{"award-number":["54\/2017"]}],"id":[{"id":"10.13039\/501100006506","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0\u2032) or 15 min (T15\u2032) after incubation, indicating its potential use as a point-of-care method as a screening strategy.<\/jats:p>","DOI":"10.3390\/ijerph192316206","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T02:56:26Z","timestamp":1670208986000},"page":"16206","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis"],"prefix":"10.3390","volume":"19","author":[{"given":"Gabriel M. C.","family":"Barros","sequence":"first","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dion\u00edsio D. A.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnaldo S.","family":"Cruz","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen K. L.","family":"Morais","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9212-2344","authenticated-orcid":false,"given":"Ana Isabela L.","family":"Sales-Moioli","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3113-7187","authenticated-orcid":false,"given":"Talita K. B.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"},{"name":"Health Sciences Research Unit: Nursing (UICISA:E), The Nursing School of Coimbra (ESEnfC), 3004-011 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melise C. D.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Sanchez-Gendriz","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5696-4114","authenticated-orcid":false,"given":"Ingridy M. P.","family":"Barbalho","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o P. Q.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge M. O.","family":"Henriques","sequence":"additional","affiliation":[{"name":"Centre for Informatics and Systems of the University of Coimbra (CISUC), Department of Informatics Engineering, University of Coimbra, 3004-531 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar A. D.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Centre for Informatics and Systems of the University of Coimbra (CISUC), Department of Informatics Engineering, University of Coimbra, 3004-531 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0937-4044","authenticated-orcid":false,"given":"Paulo","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, School of Science and Technology, New University of Lisbon, 1099-085 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00facio","family":"Gama","sequence":"additional","affiliation":[{"name":"Department of Molecular and Comparative Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21218, USA"},{"name":"Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5556-8379","authenticated-orcid":false,"given":"Ang\u00e9lica E.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Infectious Diseases, Federal University of Esp\u00edrito Santo, Vit\u00f3ria 29075-910, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karilany D.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4860-9734","authenticated-orcid":false,"given":"Leonardo J.","family":"Galv\u00e3o-Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Innovation in Health (LAIS), Hospital Universit\u00e1rio Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-8593","authenticated-orcid":false,"given":"Ricardo A. 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