{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:26:12Z","timestamp":1789471572173,"version":"build-2803163510"},"reference-count":49,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["M\u00e9diterran\u00e9e Infection 10-IAHU-03"],"award-info":[{"award-number":["M\u00e9diterran\u00e9e Infection 10-IAHU-03"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Background: The first-line diagnosis of malaria in Mali is based on the use of rapid diagnostic tests (RDT) that detect the Histidin Rich Protein 2 (HRP2) antigen specific to Plasmodium falciparum. Our study, based on a real-time polymerase chain reaction (qPCR) gold standard, aimed to describe the distribution of the Plasmodium species in each administrative region of Mali and to assess the performance of RDTs. Methods: We randomly selected 150 malaria-negative and up to 30 malaria-positive RDTs in 41 sites distributed in 9 regions of Mali. DNA extracted from the RDT nitrocellulose strip was assayed with a pan-Plasmodium qPCR. Positive samples were then analyzed with P. falciparum-, P. malariae-, P.\u00a0vivax-, or P. ovale-specific qPCRs. Results: Of the 1496 RDTs, 258 (18.6%) were positive for Plasmodium spp., of which 96.9% were P. falciparum. The P. vivax prevalence reached 21.1% in the north. RDT displayed acceptable diagnostic indices; the lower CI95% bounds of Youden indices were all \u22650.50, except in the north (Youden index 0.66 (95% CI [0.44\u20130.82]) and 0.63 (95% CI [0.33\u20130.83]. Conclusions: Overall, RDT diagnostic indices are adequate for the biological diagnosis of malaria in Mali. We recommend the use of RDTs detecting P. vivax-specific antigens in the north.<\/jats:p>","DOI":"10.3390\/ijerph21020228","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T11:19:01Z","timestamp":1707995941000},"page":"228","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of the Performance of Rapid Diagnostic Tests for Malaria Diagnosis and Mapping of Different Plasmodium Species in Mali"],"prefix":"10.3390","volume":"21","author":[{"given":"Pascal","family":"Demb\u00e9l\u00e9","sequence":"first","affiliation":[{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection (IHU), Aix Marseille Universit\u00e9, 13005 Marseille, France"},{"name":"Aix-Marseille University, IRD, AP-HM, SSA, VITROME, 13005 Marseille, France"},{"name":"Programme National de Lutte Contre le Paludisme (PNLP), Bamako BP 233, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0200-8191","authenticated-orcid":false,"given":"Mady","family":"Cissoko","sequence":"additional","affiliation":[{"name":"Programme National de Lutte Contre le Paludisme (PNLP), Bamako BP 233, Mali"},{"name":"Malaria Research and Training Center (MRTC), FMOS-FAPH, Mali-NIAID-ICER, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako, Bamako BP 1805, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adama Zan","family":"Diarra","sequence":"additional","affiliation":[{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection (IHU), Aix Marseille Universit\u00e9, 13005 Marseille, France"},{"name":"Aix-Marseille University, IRD, AP-HM, SSA, VITROME, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lassana","family":"Doumbia","sequence":"additional","affiliation":[{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection (IHU), Aix Marseille Universit\u00e9, 13005 Marseille, France"},{"name":"Laboratoire de Biologie Mol\u00e9culaire Appliqu\u00e9e (LBMA), Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako, Badalabougou, Bamako BP 423, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u00efssata","family":"Kon\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Mol\u00e9culaire Appliqu\u00e9e (LBMA), Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako, Badalabougou, Bamako BP 423, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamadou H.","family":"Magassa","sequence":"additional","affiliation":[{"name":"Programme National de Lutte Contre le Paludisme (PNLP), Bamako BP 233, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maissane","family":"Mehadji","sequence":"additional","affiliation":[{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection (IHU), Aix Marseille Universit\u00e9, 13005 Marseille, France"},{"name":"Aix-Marseille University, IRD, AP-HM, SSA, VITROME, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2679-035X","authenticated-orcid":false,"given":"Mahamadou A.","family":"Thera","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center (MRTC), FMOS-FAPH, Mali-NIAID-ICER, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako, Bamako BP 1805, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-5276","authenticated-orcid":false,"given":"St\u00e9phane","family":"Ranque","sequence":"additional","affiliation":[{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection (IHU), Aix Marseille Universit\u00e9, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"ref_1","unstructured":"(2023, March 05). 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