{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:07:12Z","timestamp":1760144832560,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T00:00:00Z","timestamp":1716595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Science and Technology in Health\/Ministry of Health (DECIT\/MS)","award":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"],"award-info":[{"award-number":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"]}]},{"name":"the Oswaldo Cruz Foundation (Fiocruz)","award":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"],"award-info":[{"award-number":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"]}]},{"name":"the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro (FAPERJ)","award":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"],"award-info":[{"award-number":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"]}]},{"name":"Health Surveillance Secretariat\/Ministry of Health (SVS\/MS)","award":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"],"award-info":[{"award-number":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"]}]},{"name":"Federal University of Roraima","award":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"],"award-info":[{"award-number":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"]}]},{"name":"MFFC and CTDR are supported by CNPq, Brazil, through Research Productivity Fellowships","award":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"],"award-info":[{"award-number":["# 402675\/2018-5","# PAEF3 - IOC-008-FIO-22-2-60","# 260003\/001169\/2020","# TED 60\/2018","# 60\/2022","# 315727\/2021-7"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Multidrug- and artemisinin-resistant (ART-R) Plasmodium falciparum\u00a0(Pf) parasites represent a challenge for malaria elimination worldwide. Molecular monitoring in the Kelch domain region (pfk13) gene allows tracking mutations in parasite resistance to artemisinin. The increase in illegal miners in the Roraima Yanomami indigenous land (YIL) could favor ART-R parasites. Thus, this study aimed to investigate ART-R in patients from illegal gold mining areas in the YIL of Roraima, Brazil. A questionnaire was conducted, and blood was collected from 48 patients diagnosed with P. falciparum or mixed malaria (Pf + P. vivax). The DNA was extracted and the pfk13 gene was amplified by PCR. The amplicons were subjected to DNA-Sanger-sequencing and the entire amplified fragment was analyzed. Among the patients, 96% (46) were from illegal mining areas of the YIL. All parasite samples carried the wild-type genotypes\/ART-sensitive phenotypes. These data reinforce the continued use of artemisinin-based combination therapies (ACTs) in Roraima, as well as the maintenance of systematic monitoring for early detection of parasite populations resistant to ART, mainly in regions with an intense flow of individuals from mining areas, such as the YIL. This is especially true when the achievement of falciparum malaria elimination in Brazil is planned and expected by 2030.<\/jats:p>","DOI":"10.3390\/ijerph21060679","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T10:14:07Z","timestamp":1716804847000},"page":"679","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Surveillance of Artemisinin-Resistant Plasmodium falciparum Parasites in Mining Areas of the Roraima Indigenous Territory in Brazil"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8999-1722","authenticated-orcid":false,"given":"Jacqueline","family":"de Aguiar-Barros","sequence":"first","affiliation":[{"name":"Malaria Control Center, Epidemiological Surveillance Department, General Health Surveillance Coordination, SESAU-RR, Roraima 69305-080, Brazil"},{"name":"Postgraduate Program in Biodiversity and Biotechnology\u2013BIONORTE Network\/Roraima Federal University (UFRR), Roraima 69310-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"Granja","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biodiversity and Biotechnology\u2013BIONORTE Network\/Roraima Federal University (UFRR), Roraima 69310-000, Brazil"},{"name":"Biodiversity Research Centre, Roraima Federal University (UFRR), Roraima 69304-000, Brazil"},{"name":"Graduate Program in Natural Resources, Federal University of Roraima (UFRR), Roraima 69304-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"de Abreu-Fernandes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil"},{"name":"Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal)\/Reference Center for Malaria in the Extra-Amazon Region of the Brazilian Ministry of Health, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Tavares","family":"de Queiroz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil"},{"name":"Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal)\/Reference Center for Malaria in the Extra-Amazon Region of the Brazilian Ministry of Health, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"da Silva e Silva","sequence":"additional","affiliation":[{"name":"Biodiversity Research Centre, Roraima Federal University (UFRR), Roraima 69304-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9963-8167","authenticated-orcid":false,"given":"Arthur Camur\u00e7a","family":"Cit\u00f3","sequence":"additional","affiliation":[{"name":"Research Support Center in Roraima (NAPRR) of the National Institute for Amazonian Research (INPA), Roraima 69301-150, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia Ketrin Almeida-de-Oliveira","family":"Mocelin","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil"},{"name":"Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal)\/Reference Center for Malaria in the Extra-Amazon Region of the Brazilian Ministry of Health, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio Tadeu","family":"Daniel-Ribeiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil"},{"name":"Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal)\/Reference Center for Malaria in the Extra-Amazon Region of the Brazilian Ministry of Health, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3522-3792","authenticated-orcid":false,"given":"Maria de F\u00e1tima","family":"Ferreira-da-Cruz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Mal\u00e1ria, Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil"},{"name":"Centro de Pesquisa, Diagn\u00f3stico e Treinamento em Mal\u00e1ria (CPD-Mal)\/Reference Center for Malaria in the Extra-Amazon Region of the Brazilian Ministry of Health, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,25]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2023). 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