{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T04:24:05Z","timestamp":1784694245441,"version":"3.55.0"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Antibiotic resistance (AR) is a critical global health threat exacerbated by complex human\u2013animal\u2013environment interactions. Aquatic environments, particularly surface water systems, can serve as reservoirs and transmission routes for AR bacteria. This study investigated the prevalence of AR E. coli in Sinking Creek, a pathogen-impacted creek in Northeast Tennessee. Water samples were collected monthly from four sites along the creek over a 6-month period. E. coli isolates were cultured, identified, and tested for susceptibility to eight antibiotics using the Kirby\u2013Bauer disk diffusion method and broth disk elution method for colistin. Data were analyzed to determine the prevalence of AR and multidrug resistance (MDR) among isolates. Of the 122 water samples, 89.3% contained E. coli. Among the 177 isolates tested, resistance was highest to ciprofloxacin (64.2%) and nitrofurantoin (62.7%), and lowest to fosfomycin (14.1%) and colistin (6.0%). Significant differences in resistance to ceftriaxone and amoxicillin\/clavulanic acid were observed between sampling sites. MDR was prevalent in 47.5% of isolates, with 5.1% resistant to seven antibiotics. The most frequent MDR patterns (6.8%) included three antibiotics: ceftriaxone, ciprofloxacin, and nitrofurantoin. The high prevalence of AR E. coli in Sinking Creek poses a significant public health risk, highlighting the need for ongoing surveillance and intervention strategies to prevent the spread of AR bacteria.<\/jats:p>","DOI":"10.3390\/ijerph21101285","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T09:46:20Z","timestamp":1727343980000},"page":"1285","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prevalence of Antimicrobial Resistant Escherichia coli from Sinking Creek in Northeast Tennessee"],"prefix":"10.3390","volume":"21","author":[{"given":"Walid Q.","family":"Alali","sequence":"first","affiliation":[{"name":"Department of Biostatistics & Epidemiology, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip","family":"Scheuerman","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health and Safety Sciences, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"McClure","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health and Safety Sciences, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achala","family":"Ghimire","sequence":"additional","affiliation":[{"name":"Department of Biostatistics & Epidemiology, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscilla","family":"Owusu-Mensah","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health and Safety Sciences, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9174-3528","authenticated-orcid":false,"given":"Jacob","family":"Schultz","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health and Safety Sciences, College of Public Health, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2790-6817","authenticated-orcid":false,"given":"Timothy Andrew","family":"Joyner","sequence":"additional","affiliation":[{"name":"Department of Geosciences, College of Arts and Sciences, East Tennessee State University, Johnson City, TN 37614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"ref_1","unstructured":"WHO (World Health Organization) (2024, August 11). Antibiotic Resistance. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/antimicrobial-resistance."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"EclinicalMedicine (2021). Antimicrobial resistance: A top ten global public health threat. eClinicalMedicine, 41, 101221.","DOI":"10.1016\/j.eclinm.2021.101221"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.20506\/rst.31.1.2098","article-title":"Antimicrobial resistance: A complex issue","volume":"31","author":"Acar","year":"2012","journal-title":"Rev. Sci. Tech."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S1473-3099(12)70317-1","article-title":"The role of the natural environment in the emergence of antibiotic resistance in gram-negative bacteria","volume":"13","author":"Wellington","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kusi, J., Ojewole, C.O., Ojewole, A.E., and Nwi-Mozu, I. (2022). Antimicrobial Resistance Development Pathways in Surface Waters and Public Health Implications. Antibiotics, 11.","DOI":"10.3390\/antibiotics11060821"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Singh, A.K., Kaur, R., Verma, S., and Singh, S. (2022). Antimicrobials and Antibiotic Resistance Genes in Water Bodies: Pollution, Risk, and Control. Front. Environ. Sci., 10.","DOI":"10.3389\/fenvs.2022.830861"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Cho, S., Jackson, C.R., and Frye, J.G. (2023). Freshwater environment as a reservoir of extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae. J. Appl. Microbiol., 134.","DOI":"10.1093\/jambio\/lxad034"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bashir, I., Lone, F.A., Bhat, R.A., Mir, S.A., Dar, Z.A., and Dar, S.A. (2020). Concerns and Threats of Contamination on Aquatic Ecosystems. Bioremediation and Biotechnology, Springer Nature.","DOI":"10.1007\/978-3-030-35691-0_1"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Nappier, S.P., Liguori, K., Ichida, A.M., Stewart, J.R., and Jones, K.R. (2020). Antibiotic Resistance in Recreational Waters: State of the Science. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17218034"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7445","DOI":"10.1021\/es060413l","article-title":"Antibiotic resistance genes as emerging contaminants: Studies in northern Colorado","volume":"40","author":"Pruden","year":"2006","journal-title":"Environ. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.scitotenv.2013.04.067","article-title":"Genetic diversity and antimicrobial resistance of Escherichia coli from Tagus estuary (Portugal)","volume":"461\u2013462","author":"Pereira","year":"2013","journal-title":"Sci. Total Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.tim.2017.09.005","article-title":"Antibiotic-Resistance Genes in Waste Water","volume":"26","author":"Karkman","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.copbio.2008.05.006","article-title":"Antibiotics and antibiotic resistance in water environments","volume":"19","author":"Baquero","year":"2008","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Suzuki, S., Pruden, A., Virta, M., and Zhang, T. (2017). Editorial: Antibiotic Resistance in Aquatic Systems. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00014"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.envint.2017.11.003","article-title":"Exposure to and colonisation by antibiotic-resistant E. coli in UK coastal water users: Environmental surveillance, exposure assessment, and epidemiological study (Beach Bum Survey)","volume":"114","author":"Leonard","year":"2018","journal-title":"Environ. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1016\/j.envpol.2009.05.051","article-title":"Environmental pollution by antibiotics and by antibiotic resistance determinants","volume":"157","author":"Martinez","year":"2009","journal-title":"Environ. Pollut."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1111\/j.1574-6976.2011.00277.x","article-title":"Analysis of antibiotic resistance regions in Gram-negative bacteria","volume":"35","author":"Partridge","year":"2011","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"S347","DOI":"10.1038\/sj.bjp.0707607","article-title":"Plasmid encoded antibiotic resistance: Acquisition and transfer of antibiotic resistance genes in bacteria","volume":"153","author":"Bennett","year":"2008","journal-title":"Br. J. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"167","DOI":"10.2147\/IDR.S48820","article-title":"Horizontal gene transfer in the human gastrointestinal tract: Potential spread of antibiotic resistance genes","volume":"7","author":"Huddleston","year":"2014","journal-title":"Infect. Drug Resist."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1128\/AEM.72.1.612-621.2006","article-title":"Presence and growth of naturalized Escherichia coli in temperate soils from Lake Superior watersheds","volume":"72","author":"Ishii","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4684","DOI":"10.1128\/AEM.00698-13","article-title":"Evidence for coexistence of distinct Escherichia coli populations in various aquatic environments and their survival in estuary water","volume":"79","author":"Berthe","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Djordjevic, S.P., Stokes, H.W., and Roy Chowdhury, P. (2013). Mobile elements, zoonotic pathogens and commensal bacteria: Conduits for the delivery of resistance genes into humans, production animals and soil microbiota. Front. Microbiol., 4.","DOI":"10.3389\/fmicb.2013.00086"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/1574-6976.12031","article-title":"Microbial source tracking markers for detection of fecal contamination in environmental waters: Relationships between pathogens and human health outcomes","volume":"38","author":"Harwood","year":"2014","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Cho, S., Hiott, L.M., Barrett, J.B., McMillan, E.A., House, S.L., Humayoun, S.B., Adams, E.S., Jackson, C.R., and Frye, J.G. (2018). Prevalence and characterization of Escherichia coli isolated from the Upper Oconee Watershed in Northeast Georgia. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0197005"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"139401","DOI":"10.1016\/j.scitotenv.2020.139401","article-title":"Effects of concentrated poultry operations and cropland manure application on antibiotic resistant Escherichia coli and nutrient pollution in Chesapeake Bay watersheds","volume":"735","author":"Amato","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3862949","DOI":"10.1155\/2019\/3862949","article-title":"Antimicrobial-Resistant Escherichia coli from Environmental Waters in Northern Colorado","volume":"2019","author":"Haberecht","year":"2019","journal-title":"J. Environ. Public Health"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"166753","DOI":"10.1016\/j.scitotenv.2023.166753","article-title":"Simultaneous stream assessment of antibiotics, bacteria, antibiotic resistant bacteria, and antibiotic resistance genes in an agricultural region of the United States","volume":"904","author":"Givens","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"997","DOI":"10.2134\/jeq2008.0077","article-title":"Occurrence and Antibiotic Resistance of Escherichia coli O157:H7 in a Watershed in North-Central Indiana","volume":"38","author":"Fincher","year":"2009","journal-title":"J. Environ. Qual."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Dom\u00ednguez, D.C., Chac\u00f3n, L.M., and Wallace, D.J. (2021). Anthropogenic Activities and the Problem of Antibiotic Resistance in Latin America: A Water Issue. Water, 13.","DOI":"10.3390\/w13192693"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bong, C.W., Low, K.Y., Chai, L.C., and Lee, C.W. (2022). Prevalence and Diversity of Antibiotic Resistant Escherichia coli from Anthropogenic-Impacted Larut River. Front. Public Health, 10.","DOI":"10.3389\/fpubh.2022.794513"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Truong, T., Hoang, T.L., Tran, L.T., Pham, T.P.T., and Le, T.-H. (2021). Prevalence of Antibiotic Resistance Genes in the Saigon River Impacted by Anthropogenic Activities. Water, 13.","DOI":"10.3390\/w13162234"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e5610","DOI":"10.7717\/peerj.5610","article-title":"Maxent estimation of aquatic Escherichia coli stream impairment","volume":"6","author":"Gilfillan","year":"2018","journal-title":"PeerJ"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"358","DOI":"10.2134\/jeq2013.05.0190","article-title":"Application of multivariate statistical methodology to model factors influencing fate and transport of fecal pollution in surface waters","volume":"43","author":"Hall","year":"2014","journal-title":"J. Environ. Qual."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.2175\/106143017X14839994523901","article-title":"Development of Multiple Regression Models to Predict Sources of Fecal Pollution","volume":"89","author":"Hall","year":"2017","journal-title":"Water Environ. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"974","DOI":"10.2134\/jeq2017.12.0474","article-title":"Canonical Variable Selection for Ecological Modeling of Fecal Indicators","volume":"47","author":"Gilfillan","year":"2018","journal-title":"J. Environ. Qual."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/jeq2.20196","article-title":"Storm sampling to assess inclement weather impacts on water quality in a karst watershed: Sinking Creek, Watauga watershed, East Tennessee","volume":"50","author":"McCurdy","year":"2021","journal-title":"J. Environ. Qual."},{"key":"ref_37","unstructured":"TDEC (Tennessee Department of Environment and Conservation) (2024, July 29). Water Quality Rules, Reports & Publications, Available online: https:\/\/www.tn.gov\/environment\/program-areas\/wr-water-resources\/water-quality\/water-quality-reports---publications.html."},{"key":"ref_38","unstructured":"USEPA (2024, March 10). Method 1603 Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified Membrane-Thermotolerant Escherichia coli Agar (Modified m-TEC), EPA 821-R-02-023, Available online: https:\/\/nepis.epa.gov\/Exe\/ZyNET.exe\/P1008MZV.txt?ZyActionD=ZyDocument&Client=EPA&Index=2000%20Thru%202005&Docs=&Query=&Time=&EndTime=&SearchMethod=1&TocRestrict=n&Toc=&TocEntry=&QField=&QFieldYear=&QFieldMonth=&QFieldDay=&UseQField=&IntQFieldOp=0&ExtQFie."},{"key":"ref_39","unstructured":"CLSI (2021). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [31st ed.]. CLSI Supplement M100."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1017\/S0950268811001038","article-title":"The role of drinking water in the transmission of antimicrobial-resistant E. coli","volume":"140","author":"Coleman","year":"2012","journal-title":"Epidemiol. Infect."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/j.scitotenv.2018.09.018","article-title":"The potential human exposure to antibiotic resistant-Escherichia coli through recreational water","volume":"650","author":"Solimini","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e357","DOI":"10.1016\/S2542-5196(19)30130-5","article-title":"Attributable sources of community-acquired carriage of Escherichia coli containing \u03b2-lactam antibiotic resistance genes: A population-based modelling study","volume":"3","author":"Dierikx","year":"2019","journal-title":"Lancet Planet. Health"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6321","DOI":"10.1016\/j.watres.2011.09.018","article-title":"Escherichia coli virulence genes profile of surface waters as an indicator of water quality","volume":"45","author":"Masters","year":"2011","journal-title":"Water Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s11270-017-3426-1","article-title":"Influence of Sewered versus Septic Systems on Watershed Exports of E. coli","volume":"228","author":"Iverson","year":"2017","journal-title":"Water Air Soil Pollut."},{"key":"ref_45","unstructured":"USEPA (2024, June 10). Septic System Impacts on Water Sources, Available online: https:\/\/www.epa.gov\/septic\/septic-system-impacts-water-sources."},{"key":"ref_46","unstructured":"CDC (2024, June 10). All Antibiotic Classes|A.R. & Patient Safety Portal, Available online: https:\/\/arpsp.cdc.gov\/profile\/antibiotic-use\/all-classes."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2540","DOI":"10.1128\/AAC.46.8.2540-2545.2002","article-title":"Trends in Antimicrobial Resistance among Urinary Tract Infection Isolates of Escherichia coli from Female Outpatients in the United States","volume":"46","author":"Thornsberry","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1086\/345754","article-title":"Susceptibility of Antimicrobial-Resistant Urinary Escherichia coli Isolates to Fluoroquinolones and Nitrofurantoin","volume":"36","author":"Karlowsky","year":"2003","journal-title":"Clin. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.watres.2017.09.043","article-title":"Nutrient loading impacts on culturable E. coli and other heterotrophic bacteria fate in simulated stream mesocosms","volume":"126","author":"Gregory","year":"2017","journal-title":"Water Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Ibekwe, A.M., Murinda, S.E., and Graves, A.K. (2011). Genetic diversity and antimicrobial resistance of Escherichia coli from human and animal sources uncovers multiple resistances from human sources. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0020819"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"336","DOI":"10.3389\/fmicb.2015.00336","article-title":"Detection of multi-drug resistant Escherichia coli in the urban waterways of Milwaukee, WI","volume":"6","author":"Kappell","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0022-0981(03)00358-7","article-title":"Identification of sources of Escherichia coli in South Carolina estuaries using antibiotic resistance analysis","volume":"298","author":"Webster","year":"2004","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Odonkor, S.T., and Addo, K.K. (2018). Prevalence of Multidrug-Resistant Escherichia coli Isolated from Drinking Water Sources. Int. J. Microbiol., 2018.","DOI":"10.1155\/2018\/7204013"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"giaa125","DOI":"10.1093\/gigascience\/giaa125","article-title":"Localized effect of treated wastewater effluent on the resistome of an urban watershed","volume":"9","author":"Thornton","year":"2020","journal-title":"GigaScience"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Sidhu, A.S., Mikolajczyk, F.N., and Fisher, J.C. (2023). Antimicrobial Resistance Linked to Septic System Contamination in the Indiana Lake Michigan Watershed. Antibiotics, 12.","DOI":"10.3390\/antibiotics12030569"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Tabut, P., Yongyod, R., Ungcharoen, R., and Kerdsin, A. (2022). The Distribution of Mobile Colistin-Resistant Genes, Carbapenemase-Encoding Genes, and Fluoroquinolone-Resistant Genes in Escherichia coli Isolated from Natural Water Sources in Upper Northeast Thailand. Antibiotics, 11.","DOI":"10.3390\/antibiotics11121760"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s13756-021-00963-2","article-title":"Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa","volume":"10","author":"Snyman","year":"2021","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e02244-16","DOI":"10.1128\/AAC.02244-16","article-title":"Prevalence of mcr-1 in the Cecal Contents of Food Animals in the United States","volume":"61","author":"Meinersmann","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"107434","DOI":"10.1016\/j.foodcont.2020.107434","article-title":"Low prevalence of mobile colistin-resistance in U.S. meat, catfish, poultry and genomic characterization of a mcr-1 positive Escherichia coli strain","volume":"118","author":"Wang","year":"2020","journal-title":"Food Control"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1017\/ice.2019.177","article-title":"Identification of four patients with colistin-resistant Escherichia coli containing the mobile colistin resistance mcr-1 gene from a single health system in Michigan","volume":"40","author":"Henig","year":"2019","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1099\/jmm.0.001012","article-title":"Multidrug- and colistin-resistant Salmonella enterica 4,[5],12:i:- sequence type 34 carrying the mcr-3.1 gene on the IncHI2 plasmid recovered from a human","volume":"68","author":"Monte","year":"2019","journal-title":"J. Med. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Gelalcha, B.D., Mohammed, R.I., Gelgie, A.E., and Kerro Dego, O. (2023). Molecular epidemiology and pathogenomics of extended-spectrum beta-lactamase producing- Escherichia coli and - Klebsiella pneumoniae isolates from bulk tank milk in Tennessee, USA. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1283165"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Mondal, A.H., Khare, K., Saxena, P., Debnath, P., Mukhopadhyay, K., and Yadav, D. (2024). A Review on Colistin Resistance: An Antibiotic of Last Resort. Microorganisms, 12.","DOI":"10.3390\/microorganisms12040772"}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/21\/10\/1285\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:04:06Z","timestamp":1760112246000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/21\/10\/1285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,26]]},"references-count":63,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["ijerph21101285"],"URL":"https:\/\/doi.org\/10.3390\/ijerph21101285","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,26]]}}}