{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:09:44Z","timestamp":1791245384345,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the European Union (NextGeneration EU) through the MUR-PNRR project SAMOTHRACE","award":["ECS00000022"],"award-info":[{"award-number":["ECS00000022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Antimicrobial resistance (AMR) poses a global public health threat. Wastewater analysis provides valuable insights into antimicrobial resistance genes (ARGs), identifying sources and trends and evaluating AMR control measures. Between February 2022 and March 2023, pre-treatment urban wastewater samples were collected weekly from treatment plants in Pantano D\u2019Arci, Siracusa, and Giarre (Sicily, Italy). Monthly composite DNA extracts were prepared by combining weekly subsamples from each site, yielding 42 composite samples\u201414 from each treatment plant. Real-time PCR analysis targeted specific ARGs, including blaSHV, erm(A), erm(B), blaOXA, blaNDM, blaVIM, blaTEM, and blaCTX-M. The preliminary findings revealed that blaERM-B, blaOXA, blaTEM, and blaCTX-M were present in all samples, with erm(B) (median value: 8.51; range: 1.67\u201330.93), blaSHV (0.78; 0.00\u20136.36), and blaTEM (0.72; 0.34\u20134.30) showing the highest relative abundance. These results underscore the importance of integrating ARG data with broader research to understand the persistence and proliferation mechanisms of ARGs in wastewater environments. Future studies should employ metagenomic analyses to profile resistomes in urban, hospital, agricultural, and farm wastewater. Comparing these profiles will help identify contamination pathways and inform the development of targeted ARG surveillance programs. Monitoring shifts in ARG abundance could signal cross-sectoral contamination, enabling more effective AMR control strategies.<\/jats:p>","DOI":"10.3390\/ijerph22030351","type":"journal-article","created":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T08:04:44Z","timestamp":1740643484000},"page":"351","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Monitoring Antibiotic Resistance in Wastewater: Findings from Three Treatment Plants in Sicily, Italy"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2023-2135","authenticated-orcid":false,"given":"Roberta","family":"Magnano San Lio","sequence":"first","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2655-8574","authenticated-orcid":false,"given":"Andrea","family":"Maugeri","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0905-5003","authenticated-orcid":false,"given":"Martina","family":"Barchitta","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Favara","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Clara","family":"La Rosa","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"La Mastra","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4405-8162","authenticated-orcid":false,"given":"Antonella","family":"Agodi","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences and Advanced Technologies \u201cGF Ingrassia\u201d, University of Catania, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"ref_1","unstructured":"O\u2019NEILL, C.B.J. (2016). Tackling Drug-Resistant Infections Globally: Final Report and Recommendations."},{"key":"ref_2","unstructured":"World Health Organization, WHO (2025, January 23). Global Antimicrobial Resistance and Use Surveillance System (GLASS) Report: Early Implementation 2020. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240005587."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"107171","DOI":"10.1016\/j.envint.2022.107171","article-title":"Systematic review of wastewater surveillance of antimicrobial resistance in human populations","volume":"162","author":"Chau","year":"2022","journal-title":"Environ. Int."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mahfouz, N., Caucci, S., Achatz, E., Semmler, T., Guenther, S., Berendonk, T.U., and Schroeder, M. (2018). High genomic diversity of multi-drug resistant wastewater Escherichia coli. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-27292-6"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.trac.2018.06.004","article-title":"Wastewater-based epidemiology biomarkers: Past, present and future","volume":"105","author":"Choi","year":"2018","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"168056","DOI":"10.1016\/j.scitotenv.2023.168056","article-title":"Combating antibiotic resistance using wastewater surveillance: Significance, applications, challenges, and future directions","volume":"908","author":"Gholipour","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4395","DOI":"10.1080\/10643389.2021.2024739","article-title":"A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater","volume":"52","author":"Keenum","year":"2022","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"14982","DOI":"10.1021\/acs.est.1c08673","article-title":"Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes","volume":"56","author":"Garner","year":"2022","journal-title":"Environ. Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1128\/AAC.00419-13","article-title":"The Comprehensive Antibiotic Resistance Database","volume":"57","author":"Waglechner","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Husna, A., Rahman, M.M., Badruzzaman, A.T.M., Sikder, M.H., Islam, M.R., Rahman, M.T., Alam, J., and Ashour, H.M. (2023). Extended-Spectrum \u03b2-Lactamases (ESBL): Challenges and Opportunities. Biomedicines, 11.","DOI":"10.3390\/biomedicines11112937"},{"key":"ref_11","first-page":"000770","article-title":"Large-scale characterization of the macrolide resistome reveals high diversity and several new pathogen-associated genes","volume":"8","author":"Lund","year":"2022","journal-title":"Microb. Genom."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Meng, M., Li, Y., and Yao, H. (2022). Plasmid-Mediated Transfer of Antibiotic Resistance Genes in Soil. Antibiotics, 11.","DOI":"10.3390\/antibiotics11040525"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1111\/j.1574-6976.2011.00273.x","article-title":"Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens","volume":"35","author":"Stokes","year":"2011","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Salam, M.A., Al-Amin, M.Y., Salam, M.T., Pawar, J.S., Akhter, N., Rabaan, A.A., and Alqumber, M.A.A. (2023). Antimicrobial Resistance: A Growing Serious Threat for Global Public Health. Healthcare, 11.","DOI":"10.20944\/preprints202305.0555.v1"},{"key":"ref_15","unstructured":"La Rosa, G., Bonadonna, L., Suffredini, E., and Istituto Superiore di Sanit\u00e0 (2025, January 23). Protocollo Della Sorveglianza di SARS-CoV-2 in Reflui urbani (SARI)\u2014Rev. 3. Available online: https:\/\/zenodo.org\/records\/5758725."},{"key":"ref_16","unstructured":"Environmental Protection Agency\u2014United States (2025, January 23). NPDES Compliance Inspection Manual, Available online: https:\/\/www.epa.gov\/sites\/default\/files\/2017-03\/documents\/npdesinspect-chapter-05.pdf."},{"key":"ref_17","unstructured":"TaqPath\u2122 qPCR Master Mix, CG. (2025, January 23). USER GUIDE For Two-Step RT-PCR in Gene Expression Experiments or Quantitative Analysis. Applied Biosystems. Available online: https:\/\/assets.fishersci.com\/TFS-Assets\/LSG\/manuals\/TaqPath_qPCR_MasterMixCG_man.pdf."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"263","DOI":"10.4103\/0974-777X.68531","article-title":"Extended-spectrum \u03b2-lactamases in Gram Negative Bacteria","volume":"2","author":"Rawat","year":"2010","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1128\/AAC.00585-11","article-title":"Genetic features of blaNDM-1-positive Enterobacteriaceae","volume":"55","author":"Poirel","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kumari, M., Verma, S., Venkatesh, V., Gupta, P., Tripathi, P., Agarwal, A., Siddiqui, S.S., Arshad, Z., and Prakash, V. (2021). Emergence of blaNDM-1 and blaVIM producing Gram-negative bacilli in ventilator-associated pneumonia at AMR Surveillance Regional Reference Laboratory in India. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0256308"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"447","DOI":"10.5455\/javar.2022.i613","article-title":"Molecular identification of blaTEM gene of extended-spectrum beta-lactamase-producing Escherichia coli from healthy pigs in Malang district, East Java, Indonesia","volume":"9","author":"Effendi","year":"2022","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.bjm.2016.04.020","article-title":"CTX-M extended-spectrum \u03b2-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals","volume":"47","author":"Bialvaei","year":"2016","journal-title":"Braz. J. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1111\/j.1469-0691.2006.01486.x","article-title":"Prevalence of erm genes encoding macrolide-lincosamide-streptogramin (MLS) resistance among clinical isolates of Staphylococcus aureus in a Turkish university hospital","volume":"12","author":"Saribas","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mukherjee, M., Laird, E., Gentry, T.J., Brooks, J.P., and Karthikeyan, R. (2021). Increased Antimicrobial and Multidrug Resistance Downstream of Wastewater Treatment Plants in an Urban Watershed. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.657353"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"120761","DOI":"10.1016\/j.watres.2023.120761","article-title":"Candidate biomarkers of antibiotic resistance for the monitoring of wastewater and the downstream environment","volume":"247","author":"Teixeira","year":"2023","journal-title":"Water Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wa\u015bko, I., Kozi\u0144ska, A., Kotlarska, E., and Baraniak, A. (2022). Clinically Relevant \u03b2-Lactam Resistance Genes in Wastewater Treatment Plants. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph192113829"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cheney, C., Johnson, J.D., Ste Marie, J.P., Gacosta, K.Y.M., Denlinger Drumm, N.B., Jones, G.D., Waite-Cusic, J., and Navab-Daneshmand, T. (2024). Resolved genomes of wastewater ESBL-producing Escherichia coli and metagenomic analysis of source wastewater samples. Microbiol. Spectr., 12.","DOI":"10.1128\/spectrum.00717-24"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Coyte, K.Z., Stevenson, C., Knight, C.G., Harrison, E., Hall, J.P.J., and Brockhurst, M.A. (2022). Horizontal gene transfer and ecological interactions jointly control microbiome stability. PLoS Biol., 20.","DOI":"10.1371\/journal.pbio.3001847"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Sun, D., Jeannot, K., Xiao, Y., and Knapp, C.W. (2019). Editorial: Horizontal Gene Transfer Mediated Bacterial Antibiotic Resistance. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.01933"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Martinez, E.P., Cepeda, M., Jovanoska, M., Bramer, W.M., Schoufour, J., Glisic, M., Verbon, A., and Franco, O.H. (2019). Seasonality of antimicrobial resistance rates in respiratory bacteria: A systematic review and meta-analysis. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0221133"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Dai, D., Brown, C., B\u00fcrgmann, H., Larsson, D.G.J., Nambi, I., Zhang, T., Flach, C.-F., Pruden, A., and Vikesland, P.J. (2022). Long-read metagenomic sequencing reveals shifts in associations of antibiotic resistance genes with mobile genetic elements from sewage to activated sludge. Microbiome, 10.","DOI":"10.1186\/s40168-021-01216-5"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.2166\/wh.2022.026","article-title":"Research needs for optimising wastewater-based epidemiology monitoring for public health protection","volume":"20","author":"Robins","year":"2022","journal-title":"J. Water Health"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Barancheshme, F., and Munir, M. (2017). Strategies to Combat Antibiotic Resistance in the Wastewater Treatment Plants. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02603"}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/22\/3\/351\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:43:34Z","timestamp":1760028214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/22\/3\/351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["ijerph22030351"],"URL":"https:\/\/doi.org\/10.3390\/ijerph22030351","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]}}}