{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T21:47:43Z","timestamp":1792532863388,"version":"4.5.0"},"reference-count":33,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T00:00:00Z","timestamp":1758326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Infectious diseases remain a global threat, with low- and middle-income countries disproportionately affected due to socio-economic and demographic vulnerabilities. Robust laboratory systems are critical for early detection, outbreak containment, and guiding effective interventions. This study aimed to map and evaluate Jordan\u2019s laboratory diagnostic network for communicable diseases, identify gaps, and recommend strategies to strengthen capacity, harmonization, and alignment with international standards. A multi-method approach was employed in 2023 through collaboration between the Jordan Center for Disease Control and the Health Care Accreditation Council. Data were collected via (i) a desktop review of 226 national and international documents; (ii) 20 key informant interviews with stakeholders from the public, private, military, veterinary, and academic sectors; and (iii) 23 field visits across 27 laboratories in four Jordanian governorates. Data were analyzed thematically and synthesized using the LABNET framework, which outlined ten core laboratory capacities. Findings were validated through a multi-sectoral national workshop with 90 participants. The mapping revealed the absence of a unified national laboratory strategic plan, with governance dispersed across multiple authorities and limited inter-sectoral coordination. Standard operating protocols (SOPs) existed for high-priority diseases such as T.B, HIV, influenza, and COVID-19 but were lacking or outdated for other notifiable diseases, particularly zoonoses. Quality management was inconsistent, with limited participation in external quality assurance programs and minimal accreditation uptake. Biosafety and biosecurity frameworks were fragmented and insufficiently enforced, while workforce shortages, high turnover, and limited specialized training constrained laboratory performance. Despite these challenges, Jordan demonstrated strengths including skilled laboratory staff, established reference centers, and international collaborations, which provide a platform for improvement. Jordan\u2019s laboratory network has foundational strengths but faces systemic challenges in policy coherence, standardization, quality assurance, and workforce capacity. Addressing these gaps requires the development of a national laboratory strategic plan, strengthened legal and regulatory frameworks, enhanced quality management and accreditation, and integrated One Health coordination across human, animal, and environmental health sectors. These measures will improve diagnostic reliability, preparedness, and alignment with the global health security agenda.<\/jats:p>","DOI":"10.3390\/ijerph22091459","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T08:37:39Z","timestamp":1758530259000},"page":"1459","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Strengthening Jordan\u2019s Laboratory Capacity for Communicable Diseases: A Comprehensive Multi-Method Mapping Toward Harmonized National Laboratories and Evidence-Informed Public Health Planning"],"prefix":"10.3390","volume":"22","author":[{"given":"Dalia Kashef","family":"Zayed","sequence":"first","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruba A.","family":"Al-Smadi","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"},{"name":"Princess Iman Center for Research and Laboratory, Royal Medical Services, Amman 11855, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Almaayteh","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"},{"name":"Princess Iman Center for Research and Laboratory, Royal Medical Services, Amman 11855, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thekryat","family":"Al-Hjouj","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"},{"name":"Princess Iman Center for Research and Laboratory, Royal Medical Services, Amman 11855, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola","family":"Hamdan","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammar Abu","family":"Ghalyoun","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Alsaleh","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq","family":"Abu Touk","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saddam Nawaf","family":"Almaseidin","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6480-9472","authenticated-orcid":false,"given":"Thaira","family":"Madi","sequence":"additional","affiliation":[{"name":"Department of Accreditation, Health Care Accreditation Council, Amman 11181, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8070-2579","authenticated-orcid":false,"given":"Samar Khaled","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Accreditation, Health Care Accreditation Council, Amman 11181, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muna","family":"Horabi","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel","family":"Belbiesi","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0200-9845","authenticated-orcid":false,"given":"Tareq L.","family":"Mukattash","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"},{"name":"Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0862-0186","authenticated-orcid":false,"given":"Ala\u2019a B.","family":"Al-Tammemi","sequence":"additional","affiliation":[{"name":"Jordan Center for Disease Control, Amman 11183, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,20]]},"reference":[{"key":"ref_1","unstructured":"Preedy, V.R., and Watson, R.R. 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