{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:59:10Z","timestamp":1785405550420,"version":"3.56.0"},"reference-count":23,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["309610\/2023-0"],"award-info":[{"award-number":["309610\/2023-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["309610\/2023-0"],"award-info":[{"award-number":["309610\/2023-0"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-01552-21"],"award-info":[{"award-number":["APQ-01552-21"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"award":["APQ-01552-21"],"award-info":[{"award-number":["APQ-01552-21"]}],"id":[{"id":"https:\/\/ror.org\/00nc55f03","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Endodontic\u2013periodontal lesions are complex conditions in which endodontic infection and periodontal breakdown coexist and may create anaerobic microbial niches along the root surface. Although smoking is a well-established modifier of periodontal disease progression and subgingival microbial ecology, its influence on the depth-related distribution of anaerobic periodontal bacteria in teeth affected by endodontic\u2013periodontal lesions remains incompletely understood. This cross-sectional study investigated the distribution patterns and co-occurrence of selected anaerobic periodontal bacteria in smokers and non-smokers with endodontic\u2013periodontal lesions, considering periodontal pocket depth and anatomical site. Subgingival samples were collected from periodontal pockets of different probing depths (3\u20134 mm, 5\u20136 mm, and \u22657 mm), as well as from healthy gingival sulci and oral mucosa, in 26 patients with endodontic\u2013periodontal lesions. The presence of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Prevotella nigrescens, and Aggregatibacter actinomycetemcomitans was assessed. Detection was performed using polymerase chain reaction (PCR). Qualitative detection frequencies and microbial co-occurrence patterns were compared between smokers and non-smokers across sites and pocket depths. Non-smokers showed higher detection of Tannerella forsythia in pockets \u2265 7 mm (p &lt; 0.05). Overall microbial co-occurrence was lower in smokers in deeper periodontal pockets, whereas detection patterns in healthy gingival sulci and oral mucosa were broadly comparable between groups. Our findings suggest that smoking may be associated with an attenuated depth-related detection pattern and reduced co-occurrence of selected anaerobic periodontal bacteria in endodontic\u2013periodontal lesions.<\/jats:p>","DOI":"10.3390\/ijerph23070860","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:06:34Z","timestamp":1782875194000},"page":"860","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Smoking and Depth-Related Anaerobic Bacteria in Endodontic\u2013Periodontal Lesions: A Pilot Study"],"prefix":"10.3390","volume":"23","author":[{"given":"C\u00e1ssio Vicente","family":"Pereira","sequence":"first","affiliation":[{"name":"School of Dentistry, Centro Universit\u00e1rio de Lavras (UNILAVRAS), Lavras 37203-593, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1659-8524","authenticated-orcid":false,"given":"Nat\u00e1lia Galv\u00e3o","family":"Garcia","sequence":"additional","affiliation":[{"name":"School of Dentistry, Centro Universit\u00e1rio de Lavras (UNILAVRAS), Lavras 37203-593, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4069-4508","authenticated-orcid":false,"given":"Douglas Campideli","family":"Fonseca","sequence":"additional","affiliation":[{"name":"School of Dentistry, Centro Universit\u00e1rio de Lavras (UNILAVRAS), Lavras 37203-593, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2684-7739","authenticated-orcid":false,"given":"Pedro Gustavo","family":"Machado","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Health Sciences (FCS), Federal University of Lavras (UFLA), Lavras 37203-202, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele de F\u00e1tima","family":"Rezende","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Health Sciences (FCS), Federal University of Lavras (UFLA), Lavras 37203-202, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Ferreira Mattos","family":"Alc\u00e2ntara","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Health Sciences (FCS), Federal University of Lavras (UFLA), Lavras 37203-202, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9253-3108","authenticated-orcid":false,"given":"Eric Francelino","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Health Sciences (FCS), Federal University of Lavras (UFLA), Lavras 37203-202, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0502-2554","authenticated-orcid":false,"given":"Luciano Jos\u00e9","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Health Sciences (FCS), Federal University of Lavras (UFLA), Lavras 37203-202, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1902\/jop.1972.43.4.202","article-title":"The relationship of endodontic-periodontic lesions","volume":"43","author":"Simon","year":"1972","journal-title":"J. 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