{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:09:29Z","timestamp":1785506969210,"version":"3.56.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["FF-WU67-06"],"award-info":[{"award-number":["FF-WU67-06"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]},{"award":["FF-WU67-06"],"award-info":[{"award-number":["FF-WU67-06"]}],"id":[{"id":"https:\/\/ror.org\/000025p04","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010034","name":"Walailak University","doi-asserted-by":"publisher","award":["WU-CIA-04 505\/2025"],"award-info":[{"award-number":["WU-CIA-04 505\/2025"]}],"id":[{"id":"10.13039\/501100010034","id-type":"DOI","asserted-by":"publisher"}]},{"award":["WU-CIA-04 505\/2025"],"award-info":[{"award-number":["WU-CIA-04 505\/2025"]}],"id":[{"id":"https:\/\/ror.org\/04b69g067","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Environ. Res. Public Health","IJERPH"],"abstract":"<jats:p>Toxocara spp. infection is a neglected zoonotic disease associated with exposure to companion animals and contaminated environments. However, information regarding its seroprevalence and associated risk factors among cat owners in Thailand, particularly in remote island communities, remains limited. This cross-sectional study aimed to determine anti-Toxocara spp. IgG seropositivity and identify associated risk factors among cat owners in Koh Yao District, Phang Nga Province, Thailand. A total of 291 cat owners participated in the study. Sociodemographic characteristics, health-related behaviors, serum anti-Toxocara spp. IgG antibodies, and hematological parameters were collected. Univariate and multivariable logistic regression analyses were performed to identify factors associated with seropositivity. The overall prevalence of anti-Toxocara spp. IgG seropositivity was 34.36%. In the univariate analysis, failure to wash hands after cat contact was significantly associated with higher odds of seropositivity (odds ratio [OR] = 1.96, 95% confidence interval [CI]: 1.16\u20133.33; p = 0.011). This association remained significant in the multivariable logistic regression model. These findings indicate substantial exposure to Toxocara spp. among cat owners in this island community and highlight the importance of personal hygiene, particularly handwashing after cat contact, in reducing exposure to infective Toxocara eggs. Targeted health education programs and preventive interventions should be strengthened to reduce the risk of Toxocara spp. exposure in endemic communities.<\/jats:p>","DOI":"10.3390\/ijerph23081007","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T13:23:20Z","timestamp":1785504200000},"page":"1007","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence and Risk Factors Associated with Anti-Toxocara spp. IgG Seropositivity Among Cat Owners in an Island Community: A Cross-Sectional Study in Koh Yao District, Phang Nga Province, Thailand"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4976-3274","authenticated-orcid":false,"given":"Prasit","family":"Na-Ek","sequence":"first","affiliation":[{"name":"Department of Medical Science, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4826-9474","authenticated-orcid":false,"given":"Chuchard","family":"Punsawad","sequence":"additional","affiliation":[{"name":"Department of Medical Science, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8308-9930","authenticated-orcid":false,"given":"Aulia Rahmi","family":"Pawestri","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang 65145, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6894-8955","authenticated-orcid":false,"given":"Udomsak","family":"Narkkul","sequence":"additional","affiliation":[{"name":"Department of Medical Science, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1179\/136485910X12607012373957","article-title":"Human toxocariasis: Diagnosis, worldwide seroprevalences and clinical expression of the systemic and ocular forms","volume":"104","author":"Hirata","year":"2010","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e14","DOI":"10.1016\/S1473-3099(17)30331-6","article-title":"Human toxocariasis","volume":"18","author":"Ma","year":"2018","journal-title":"Lancet. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.vetpar.2011.07.014","article-title":"Role of pet dogs and cats in the transmission of helminthic zoonoses in Europe, with a focus on echinococcosis and toxocarosis","volume":"182","author":"Deplazes","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/bs.apar.2020.01.017","article-title":"Chapter Twenty-Eight\u2014Global prevalence of Toxocara infection in dogs","volume":"Volume 109","author":"Bowman","year":"2020","journal-title":"Advances in Parasitology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"392","DOI":"10.5455\/javar.2024.k788","article-title":"Prevalence and molecular analysis of Toxocara cati in Baghdad Province","volume":"11","author":"Alani","year":"2024","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_6","first-page":"550","article-title":"Survey of the prevalence of Toxocara cati in stray cats in Isfahan city, Iran by PCR method","volume":"34","author":"Torkan","year":"2017","journal-title":"Trop. Biomed."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chan, J.M., Chan, H., and Flores, M.J.C. (2023). Domesticated Cats as a Source of Environmental Contamination with Toxocara cati and Other Soil-Transmitted Helmminth Eggs in Urban Area, Manila-Philiphines. Insights Public Health J., 4.","DOI":"10.20884\/1.iphj.2023.4.1.9288"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bonilla-Aldana, J.L., Espinosa-Nu\u00f1ez, A.C., Bonilla-Aldana, D.K., and Rodriguez-Morales, A.J. (2024). Toxocara cati Infection in Cats (Felis catus): A Systematic Review and Meta-Analysis. Animals, 14.","DOI":"10.3390\/ani14071022"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1017\/S0022149X16000559","article-title":"The occurrence of Toxocara species in naturally infected broiler chickens revealed by molecular approaches","volume":"91","author":"Zibaei","year":"2017","journal-title":"J. Helminthol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rostami, A., Riahi, S.M., Holland, C.V., Taghipour, A., Khalili-Fomeshi, M., Fakhri, Y., Omrani, V.F., Hotez, P.J., and Gasser, R.B. (2019). Seroprevalence estimates for toxocariasis in people worldwide: A systematic review and meta-analysis. PLoS Neglected Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007809"},{"key":"ref_11","first-page":"e00101","article-title":"Human toxocariasis","volume":"38","author":"Padyala","year":"2025","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1111\/tmi.12607","article-title":"Identification of immunodominant antigens for the laboratory diagnosis of toxocariasis","volume":"20","author":"Zhan","year":"2015","journal-title":"Trop. Med. Int. Health"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e28","DOI":"10.1590\/s1678-9946201860028","article-title":"Seroprevalence of Toxoplasma gondii and Toxocara canis in a human rural population of Southern Rio Grande do Sul","volume":"60","author":"Araujo","year":"2018","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.actatropica.2015.03.018","article-title":"Seroprevalence, disease awareness, and risk factors for Toxocara canis infection among primary schoolchildren in Makoko, an urban slum community in Nigeria","volume":"146","author":"Gyang","year":"2015","journal-title":"Acta Trop."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Fu, C.-J., Chuang, T.-W., Lin, H.-S., Wu, C.-H., Liu, Y.-C., Langinlur, M.K., Lu, M.-Y., Hsiao, W.W.-W., and Fan, C.-K. (2014). Seroepidemiology of Toxocara canis infection among primary schoolchildren in the capital area of the Republic of the Marshall Islands. BMC Infect. Dis., 14.","DOI":"10.1186\/1471-2334-14-261"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.4269\/ajtmh.17-0542","article-title":"Toxocara Seroprevalence and Associated Risk Factors in the United States","volume":"97","author":"Berrett","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/bs.apar.2020.01.012","article-title":"Toxocara: Seroprevalence in Mexico","volume":"109","year":"2020","journal-title":"Adv. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Nicoletti, A., Cicero, C.E., Mantella, A., Giuliano, L., Rascun\u00e0, C., Paradisi, V., Bartoloni, A., Zappia, M., and Sofia, V. (2019). Seroprevalence of Toxocara Canis in the city of Catania, Italy. Mediterr. J. Hematol. Infect. Dis., 11.","DOI":"10.4084\/mjhid.2019.031"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"155","DOI":"10.18683\/germs.2018.1143","article-title":"Soil contamination by Toxocara canis and human seroprevalence in the Attica region, Greece","volume":"8","author":"Papavasilopoulos","year":"2018","journal-title":"Germs"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s41182-020-00211-0","article-title":"Seroprevalence of Toxocara canis infection and associated risk factors among primary schoolchildren in rural Southern Thailand","volume":"48","author":"Phasuk","year":"2020","journal-title":"Trop. Med. Health"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1186\/s13071-016-1780-2","article-title":"Seroprevalence of fascioliasis, toxocariasis, strongyloidiasis and cysticercosis in blood samples diagnosed in Medic Medical Center Laboratory, Ho Chi Minh City, Vietnam in 2012","volume":"9","author":"Nguyen","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_22","first-page":"551","article-title":"Toxocara egg soil contamination and its seroprevalence among public school children in Los Ba\u00f1os, Laguna, Philippines","volume":"44","author":"Fajutag","year":"2013","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Stull, J.W., Peregrine, A.S., Sargeant, J.M., and Weese, J.S. (2012). Household knowledge, attitudes and practices related to pet contact and associated zoonoses in Ontario, Canada. BMC Public Health, 12.","DOI":"10.1186\/1471-2458-12-553"},{"key":"ref_24","first-page":"31","article-title":"Gastrointestinal parasites of dogs and cats in a refuge in Nakhon Nayok, Thailand","volume":"45","author":"Rojekittikhun","year":"2014","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1292\/jvms.16-0293","article-title":"Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand","volume":"78","author":"Pumidonming","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Eslahi, A.V., Badri, M., Khorshidi, A., Majidiani, H., Hooshmand, E., Hosseini, H., Taghipour, A., Foroutan, M., Pestehchian, N., and Firoozeh, F. (2020). Prevalence of Toxocara and Toxascaris infection among human and animals in Iran with meta-analysis approach. BMC Infect. Dis., 20.","DOI":"10.1186\/s12879-020-4759-8"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Gillespie, S., and Bradbury, R.S. (2017). A Survey of Intestinal Parasites of Domestic Dogs in Central Queensland. Trop. Med. Infect. Dis., 2.","DOI":"10.3390\/tropicalmed2040060"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Merigueti, Y.F.F.B., Giuffrida, R., Silva, R.C.d., Kmetiuk, L.B., Santos, A.P.D., Biondo, A.W., and Santar\u00e9m, V.A. (2022). Dog and Cat Contact as Risk Factor for Human Toxocariasis: Systematic Review and Meta-Analysis. Front. Public Health, 10.","DOI":"10.3389\/fpubh.2022.854468"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"50","DOI":"10.26444\/aaem\/146318","article-title":"Serosurvey of anti-Toxocara antibodies and associated risk factors in domestic dogs and cats owners in Karaj, Alborz Province of Iran","volume":"29","author":"Zibaei","year":"2022","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_30","first-page":"265","article-title":"Seroepidemiological Study of Toxocariasis in the Owners of Domestic Cats and Dogs in Mashhad, Northeastern Iran","volume":"11","author":"Berenji","year":"2016","journal-title":"Iran. J. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s41182-022-00425-4","article-title":"Seroprevalence of anti-Toxocara canis antibodies and associated risk factors among dog owners in the rural community of Nakhon Si Thammarat province, southern Thailand","volume":"50","author":"Narkkul","year":"2022","journal-title":"Trop. Med. Health"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.jmii.2025.12.002","article-title":"Toxocara seroprevalence among suspected patients across different geographical regions in Vietnam: A retrospective study","volume":"59","author":"Que","year":"2025","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Punsawad, C., Na-ek, P., Narkkul, U., Rattanaburi, C., Kongsuk, A., Plub-on, T., Scholand, S.J., and Phasuk, N. (2026). Prevalence and Risk Factors of Soil-Transmitted Helminth Infections on Koh Yao Islands, Southern Thailand: A Community-Based Cross-Sectional Survey. Int. J. Environ. Res. Public Health, 23.","DOI":"10.3390\/ijerph23050595"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Na-Ek, P., Narkkul, U., Phasuk, N., Scholand, S.J., and Punsawad, C. (2026). Seroprevalence and Associated Factors of Anti-Strongyloides spp. IgG Among Primary School Children in Southern Thailand. Pathogens, 15.","DOI":"10.3390\/pathogens15060566"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Narkkul, U., Na-ek, P., Pawestri, A.R., and Punsawad, C. (2025). Prevalence of intestinal parasite contamination in raw vegetables and school soil samples in Koh Yao, Phang Nga Province, southern Thailand. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0320929"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100353","DOI":"10.1016\/j.onehlt.2021.100353","article-title":"One Health approach on human seroprevalence of anti-Toxocara antibodies, Toxocara spp. eggs in dogs and sand samples between seashore mainland and island areas of southern Brazil","volume":"13","author":"Delai","year":"2021","journal-title":"One Health"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e46235","DOI":"10.1097\/MD.0000000000046235","article-title":"Seroprevalence of Toxocara spp. infection and its associated factors among children in Vietnam: A cross-sectional study","volume":"104","author":"Huynh","year":"2025","journal-title":"Medicine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s40249-025-01312-w","article-title":"Prevalence of Toxocara infection and associated risk factors: A cross-sectional study in Zhejiang, China","volume":"14","author":"Yang","year":"2025","journal-title":"Infect. Dis. Poverty"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1016\/j.envpol.2018.07.087","article-title":"Toxocara eggs in public places worldwide\u2014A systematic review and meta-analysis","volume":"242","author":"Fakhri","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s40249-018-0437-0","article-title":"Toxocariasis: A silent threat with a progressive public health impact","volume":"7","author":"Chen","year":"2018","journal-title":"Infect. Dis. Poverty"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.vetpar.2012.12.035","article-title":"Veterinary and public health aspects of Toxocara spp.","volume":"193","author":"Overgaauw","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00430-021-00723-4","article-title":"Profiles of CD4+, CD8+, and regulatory T cells and circulating cytokines in hookworm-infected children in southern Thailand","volume":"211","author":"Phasuk","year":"2022","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.iac.2015.05.003","article-title":"Eosinophilia in Infectious Diseases","volume":"35","author":"Nutman","year":"2015","journal-title":"Immunol. Allergy Clin. N. Am."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/nri2992","article-title":"Diversity and dialogue in immunity to helminths","volume":"11","author":"Allen","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.jaci.2017.10.042","article-title":"Multifaceted roles of basophils in health and disease","volume":"142","author":"Karasuyama","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1128\/JCM.00001-09","article-title":"Development and Evaluation of a Sensitive and Specific Assay for Diagnosis of Human Toxocariasis by Use of Three Recombinant Antigens (TES-26, TES-30USM, and TES-120)","volume":"47","author":"Mohamad","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.actatropica.2008.01.008","article-title":"Application of Toxocara canis excretory\u2013secretory antigens and IgG subclass antibodies (IgG1-4) in serodiagnostic assays of human toxocariasis","volume":"106","author":"Watthanakulpanich","year":"2008","journal-title":"Acta Trop."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2907585","DOI":"10.1155\/jotm\/2907585","article-title":"Soil-Transmitted Helminth Infections and Associated Risk Factors Among Primary Schoolchildren in the Koh Yao Islands of Southern Thailand","volume":"2025","author":"Phasuk","year":"2025","journal-title":"J. Trop. Med."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/23\/8\/1007\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T13:28:28Z","timestamp":1785504508000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/23\/8\/1007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,31]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijerph23081007"],"URL":"https:\/\/doi.org\/10.3390\/ijerph23081007","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,31]]}}}