{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:01:57Z","timestamp":1790262117941,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Lead (Pb) is a common environmental contaminant found in soils. Unlike other metals, Pb has no biological role, and is potentially toxic to microorganisms. Effects of low (1 ppm) and high (500-2000) levels of lead (Pb) upon the soil microbial community was investigated by the PCR\/DGGE analysis of the 16S and nirK gene markers, indicative of general microbial community and denitrifying community, respectively. Community analysis by use of those markers had shown that Pb has detectable effects upon the community diversity even at the lowest concentration tested. Analysis of sample diversity and similarity between the samples suggested that there are several thresholds crossed as metal concentration increase, each causing a substantial change in microbial diversity. Preliminary data obtained in this study suggest that the denitrifying microbial community adapts to elevated levels of Pb by selecting for metal-resistant forms of nitrite reductases.<\/jats:p>","DOI":"10.3390\/ijerph5050450","type":"journal-article","created":{"date-parts":[[2008,12,28]],"date-time":"2008-12-28T09:09:16Z","timestamp":1230455356000},"page":"450-456","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":203,"title":["Effects of Heavy Metal Contamination upon Soil Microbes: Lead-induced Changes in General and Denitrifying Microbial Communities as Evidenced by Molecular Markers"],"prefix":"10.3390","volume":"5","author":[{"given":"Dmitri","family":"Sobolev","sequence":"first","affiliation":[{"name":"Department of Biology, P. O. Box 18540, College of Science, Engineering and Technology, Jackson State University, 1400 J.R. Lynch St.., Jackson, MS 39217 U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Begonia","sequence":"additional","affiliation":[{"name":"Department of Biology, P. O. Box 18540, College of Science, Engineering and Technology, Jackson State University, 1400 J.R. Lynch St.., Jackson, MS 39217 U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s00128-004-0528-4","article-title":"Phosphatase activity and populations of microorganisms from cadmium and lead-contaminated soils","volume":"73","author":"Begonia","year":"2004","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_2","unstructured":"Beveridge, TJ, and Doyle, RJ (1989). Metal Ions and Bacteria, John Wiley & Sons."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1128\/jb.127.3.1502-1518.1976","article-title":"Uptake and retention of metals by cell walls of Bacillus subtilis","volume":"127","author":"Beveridge","year":"1976","journal-title":"J. Bacteriol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.aquatox.2004.11.012","article-title":"Mechanism of acute silver toxicity in marine invertebrates","volume":"72","author":"Bianchini","year":"2005","journal-title":"Aquatic Toxicol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1128\/AEM.64.10.3769-3775.1998","article-title":"Development of PCR primer systems for amplification of nitrite reductase genes (nirk and nirs) to detect denitrifying bacteria in environmental samples","volume":"64","author":"Braker","year":"1998","journal-title":"Appl. Envir. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.femsec.2004.11.002","article-title":"Phylogenetic analysis of nitric oxide reductase gene homologues from aerobic ammonia-oxidizing bacteria","volume":"52","author":"Casciotti","year":"2005","journal-title":"FEMS Microbiol. Ecol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ecoleng.2004.07.005","article-title":"Plant effects on microbial assemblages and remediation of acidic coal pile runoff in mesocosm treatment wetlands","volume":"23","author":"Collins","year":"2004","journal-title":"Ecol. Engineering"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1128\/aem.58.5.1592-1600.1992","article-title":"Heavy metals alter the electrokinetic properties of bacteria, yeasts, and clay minerals","volume":"58","author":"Collins","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/j.gca.2004.09.013","article-title":"Effects of sediment iron mineral composition on microbially mediated changes in divalent metal speciation: Importance of ferrihydrite","volume":"69","author":"Cooper","year":"2005","journal-title":"Geochim. Cosmochim. Acta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1002\/qj.49709640815","article-title":"The influence of nitrogen oxides on the atmospheric ozone content","volume":"96","author":"Crutzen","year":"1970","journal-title":"Quart. Rev. J. Meteorol. Soc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1399-302X.2005.00218.x","article-title":"Isolation of silver and antibiotic-resistant Enterobacter cloacae from teeth","volume":"20","author":"Davis","year":"2005","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1128\/aem.62.8.2970-2977.1996","article-title":"Development of metal tolerance in soil bacterial communities exposed to experimentally increase metal levels","volume":"62","author":"Baath","year":"1996","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0929-1393(95)00086-0","article-title":"Thymidine and leucine incorporation into bacteria experimentally contaminated with heavy metals","volume":"3","author":"Baath","year":"1996","journal-title":"Appl. Soil. Ecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/319109a0","article-title":"Future global warming from atmospheric trace gases","volume":"319","author":"Dickinson","year":"1986","journal-title":"Nature"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0038-0717(79)90007-5","article-title":"Effects of lead on the soil bacterial microflora","volume":"1","author":"Doelman","year":"1979","journal-title":"Soil Biol. Biochem"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/BF02184325","article-title":"Short-term and long-term effects of Cd, Cr, Cu, Ni, Pb, and Zn on microbial respiration in relation to abiotic soil factors","volume":"79","author":"Doelman","year":"1984","journal-title":"Plant Soil"},{"key":"ref_17","unstructured":"Allen, HE, Huang, CP, Bailey, GW, and Bowers, AR (1995). Metal speciation and contamination of soil, CRC Press."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.chemosphere.2004.12.026","article-title":"Patterns of metals and arsenic poisoning in Vibrio fischeri","volume":"60","author":"Fulladosa","year":"2005","journal-title":"Chemosphere"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.chemosphere.2004.08.007","article-title":"Study on the toxicity of binary equitoxic mixtures of metals using the luminescent bacteria Vibrio fischeri as a biological target","volume":"58","author":"Fulladosa","year":"2005","journal-title":"Chemosphere"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5488","DOI":"10.1128\/AEM.66.12.5488-5491.2000","article-title":"Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition","volume":"66","author":"Griffiths","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.scitotenv.2004.09.005","article-title":"Microbiology of a wetland ecosystem constructed to remediate mine drainage from a heavy metal mine","volume":"338","author":"Hallberg","year":"2005","journal-title":"Science Total Environ"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1016\/j.gca.2004.11.006","article-title":"Resolving and modeling the effects of Fe and Mn redox cycling on trace metal behavior in a seasonally anoxic lake","volume":"69","author":"Smith","year":"2005","journal-title":"Geochim. Cosmochim. Acta."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/BF02406152","article-title":"Microbial populations and enzyme activity in soil treated with heavy metals","volume":"95","author":"Hemida","year":"1997","journal-title":"Water Air Soil Pollut"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00248-005-0144-9","article-title":"Improved strategy for comparing microbial assemblage fingerprints","volume":"51","author":"Hewson","year":"2006","journal-title":"Microbial Ecology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1016\/j.soilbio.2004.07.003","article-title":"Soil moisture pre-treatment effects on enzyme activities as indicators of heavy metal-contaminated and reclaimed soils","volume":"36","author":"Hinojosa","year":"2004","journal-title":"Soil Biol. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S0038-0717(02)00055-X","article-title":"Heavy metals tolerance of soil denitrifying communities: N2O dynamics","volume":"34","author":"Bechmann","year":"2002","journal-title":"Soil Biol. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/S0141-0229(03)00134-0","article-title":"Polypeptides for heavy-metal biosorption: capacity and specificity of two heterogeneous MerP proteins","volume":"33","author":"Huang","year":"2003","journal-title":"Enzyme Microbial Technol"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1016\/0016-7037(92)90353-K","article-title":"Pyritization of trace metals in anoxic marine sediments","volume":"56","author":"Morse","year":"1992","journal-title":"Geochim. Cosmochim. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1006\/enrs.2000.4228","article-title":"Fine-scale tissue distribution of cadmium, inorganic mercury, and methylmercury in nymphs of the burrowing mayfly Hexagenia rigida studied by whole-body autoradiography","volume":"85","author":"Inza","year":"2001","journal-title":"Environmental Res"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1007\/s003740000268","article-title":"Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil","volume":"32","author":"Kandeler","year":"2000","journal-title":"Biol. Fertil. Soils"},{"key":"ref_31","unstructured":"Oehme, FW (1978). Toxicity of Heavy Metals in the Environment, Marcel Dekker Inc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1130\/0091-7613(1993)021<1103:MSAMPB>2.3.CO;2","article-title":"Metal sorption and mineral precipitation by bacteria in two Amazonian river systems: Rio Solimoes and Rio Negro, Brazil","volume":"21","author":"Konhauser","year":"1993","journal-title":"Geology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3109\/10408417709102311","article-title":"Lipids in bacterial taxonomy- A taxonomist\u2019s view","volume":"5","author":"Lechevalier","year":"1977","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1128\/am.28.1.80-85.1974","article-title":"Comparison of rapid methods for analysis of bacterial fatty acids","volume":"28","author":"Moss","year":"1974","journal-title":"Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/aem.59.3.695-700.1993","article-title":"Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA","volume":"59","author":"Muyzer","year":"1993","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0378-1097(03)00325-2","article-title":"Diversity of mercury resistance determinants among Bacillus strains isolated from sediment of Minamata Bay","volume":"223","author":"Narita","year":"2003","journal-title":"FEMS Microbiol. Lett"},{"key":"ref_37","first-page":"431","article-title":"Limits to protect people eating cadmium in rice, based on epidemiological studies","volume":"21","author":"Nogawa","year":"1987","journal-title":"Trace Subst. Environ. Health."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/5\/5\/450\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:21:19Z","timestamp":1760221279000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/5\/5\/450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,31]]},"references-count":37,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2008,12]]}},"alternative-id":["ijerph5050450"],"URL":"https:\/\/doi.org\/10.3390\/ijerph5050450","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,31]]}}}