{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:03:25Z","timestamp":1791007405214,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T00:00:00Z","timestamp":1331683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern Mumbai, India in March and October, 2006 were tested for seven different enteric viruses. Among the 218 specimens tested, 95 (43.6%) were positive, 73 (76.8%) for a single virus and 22 (23.2%) for multiple viruses. Single viral infections in both, March and October showed predominance of enterovirus (EV, 33.3% and 40%) and rotavirus A (RVA, 33.3% and 25%). The other viruses detected in these months were norovirus (NoV, 12.1% and 10%), rotavirus B (RVB, 12.1% and 10%), enteric adenovirus (AdV, 6.1% and 7.5%), Aichivirus (AiV, 3% and 7.5%) and human astrovirus (HAstV, 3% and 0%). Mixed viral infections were largely represented by two viruses (84.6% and 88.9%), a small proportion showed presence of three (7.7% and 11%) and four (7.7% and 0%) viruses in the two outbreaks. Genotyping of the viruses revealed predominance of RVA G2P[4], RVB G2 (Indian Bangladeshi lineage), NoV GII.4, AdV-40, HAstV-8 and AiV B types. VP1\/2A junction region based genotyping showed presence of 11 different serotypes of EVs. Although no virus was detected in the tested water samples, examination of both water and sewage pipelines in gastroenteritis affected localities indicated leakages and possibility of contamination of drinking water with sewage water. Coexistence of multiple enteric viruses during the two outbreaks of gastroenteritis emphasizes the need to expand such investigations to other parts of India.<\/jats:p>","DOI":"10.3390\/ijerph9030895","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T14:17:19Z","timestamp":1331734639000},"page":"895-915","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Diversity in the Enteric Viruses Detected in Outbreaks of Gastroenteritis from Mumbai, Western India"],"prefix":"10.3390","volume":"9","author":[{"given":"Shobha","family":"Chitambar","sequence":"first","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varanasi","family":"Gopalkrishna","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeti","family":"Chhabra","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Patil","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsha","family":"Verma","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anismrita","family":"Lahon","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Arora","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaishali","family":"Tatte","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujata","family":"Ranshing","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganesh","family":"Dhale","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra","family":"Kolhapure","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Tikute","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagannath","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Department of Pathology, Gokuldas Tejpal Hospital, Lokmanya Tilak Marg, Fort, Mumbai 400001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Bhardwaj","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sir Jamshedjee Jeejeebhoy Hospital, Byculla, Mumbai 400008, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulbha","family":"Akarte","sequence":"additional","affiliation":[{"name":"Department of Preventive and Social Medicine, Sir Jamshedjee Jeejeebhoy Hospital, Byculla, Mumbai 400008, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sashikant","family":"Pawar","sequence":"additional","affiliation":[{"name":"Department of Preventive and Social Medicine, Sir Jamshedjee Jeejeebhoy Hospital, Byculla, Mumbai 400008, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2012,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/ncpgasthep1264","article-title":"Mechanisms of infectious diarrhea","volume":"5","author":"Navaneethan","year":"2008","journal-title":"Nat. Clin. Pract. Gastroenterol. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1046\/j.1469-0691.2003.00560.x","article-title":"Viruses causing gastroenteritis","volume":"9","author":"Wilhelmi","year":"2003","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/jmv.20445","article-title":"Identification of enteroviral infection among infants and children admitted to hospital with acute gastroentritis in Ho Chi Minh City, Vietnam","volume":"77","author":"Phan","year":"2005","journal-title":"J. Med. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.3201\/eid1608.100087","article-title":"Picornavirus salivirus\/klassevirus in children with diarrhea, China","volume":"16","author":"Shan","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1007\/s00705-011-1006-z","article-title":"Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG).","volume":"156","author":"Matthijnssens","year":"2011","journal-title":"Arch. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.jcv.2006.02.009","article-title":"RT-PCR based diagnosis revealed importance of human group B rotavirus infection in childhood diarrhoea","volume":"36","author":"Barman","year":"2006","journal-title":"J. Clin. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1002\/jmv.21613","article-title":"Detection of group B rotavirus in an adult with acute gastroenteritis in Yangon, Myanmar","volume":"81","author":"Aung","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_8","first-page":"1","article-title":"Updated norovirus outbreak management and disease prevention guidelines","volume":"60","author":"Hall","year":"2011","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1128\/jcm.28.7.1525-1529.1990","article-title":"Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986","volume":"28","author":"Brown","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1002\/jmv.21331","article-title":"Identification and characterization of enteric adenoviruses in infants and children hospitalized for acute gastroenteritis","volume":"81","author":"Verma","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_11","unstructured":"Fields, B.N., Knipe, D.M., Howley, P.M., Chanock, R.M., Monath, T.P., Melnick, J.L., Roizman, B., and Straus, S.E. (2007). Fields Virology, Lippincott, Williams & Wilkins. [5th]."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.virusres.2007.04.001","article-title":"Molecular identification of 13 new enterovirus types, EV79\u201388, EV97, and EV100\u2013101, members of the species human enterovirus B","volume":"128","author":"Oberste","year":"2007","journal-title":"Virus Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1128\/JCM.02140-07","article-title":"Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients","volume":"46","author":"Lorrot","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.meegid.2006.02.001","article-title":"Molecular epidemiology of human astrovirus infections in Kolkata, India","volume":"6","author":"Bhattacharya","year":"2006","journal-title":"Infect. Genet. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1002\/jmv.21458","article-title":"Epidemiological, clinical and molecular features of norovirus infections in western India","volume":"81","author":"Chhabra","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S147","DOI":"10.1086\/605031","article-title":"Multicenter, hospital-based surveillance of rotavirus disease and strains among Indian children","volume":"200","author":"Kang","year":"2009","journal-title":"J. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1002\/jmv.21708","article-title":"Characterization of group A rotavirus infections in adolescents and adults from Pune, India: 1993-1996 and 2004-2007","volume":"82","author":"Tatte","year":"2010","journal-title":"J. Med. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.meegid.2010.01.008","article-title":"Astrovirus associated acute gastroenteritis in western India: predominance of dual serotype strains","volume":"10","author":"Verma","year":"2010","journal-title":"Infect. Genet. Evol."},{"key":"ref_19","unstructured":"Available online:http:\/\/en.wikipedia.org\/wiki\/Mumbai."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1128\/jcm.28.6.1300-1307.1990","article-title":"Removal of inhibitory substances from human faecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reaction","volume":"28","author":"Wilde","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1128\/jcm.30.6.1365-1373.1992","article-title":"Identification of group A rotavirus gene 4 types by polymerase chain reaction","volume":"30","author":"Gentsch","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1002\/jmv.21228","article-title":"Enhancement of detection and quantification of rotavirus in stool using a modified real time RT-PCR assay","volume":"80","author":"Freeman","year":"2008","journal-title":"J. Med. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1128\/jcm.33.1.64-71.1995","article-title":"Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization","volume":"33","author":"Ando","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1002\/jmv.10145","article-title":"Foodborne outbreak caused by a Norwalk- like virus in India","volume":"67","author":"Girish","year":"2002","journal-title":"J. Med. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2659","DOI":"10.1128\/jcm.28.12.2659-2667.1990","article-title":"Polymerase chain reaction for detection of adenoviruses in stool samples","volume":"28","author":"Allard","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1128\/jcm.33.4.797-801.1995","article-title":"Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing","volume":"33","author":"Noel","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3201\/eid1502.080865","article-title":"Enteroviruses in patients with acute encephalitis, Uttar Pradesh, India","volume":"15","author":"Sapkal","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2955","DOI":"10.1128\/JCM.38.8.2955-2961.2000","article-title":"Application of a reverse transcription-PCR for identification and differentiation of aichi virus, a new member of the Picornavirus family associated with gastroenteritis in humans","volume":"38","author":"Yamashita","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0166-0934(01)00404-9","article-title":"Genogroup-specific PCR primers for detection of Norwalk-like viruses","volume":"100","author":"Kojima","year":"2002","journal-title":"J. Virol. Methods."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1128\/JCM.37.5.1288-1293.1999","article-title":"Typing of human enteroviruses by partial sequencing of VP1","volume":"37","author":"Oberste","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","first-page":"760","article-title":"Molecular characterization of hepatitis A virus from a large outbreak from Kerala, India","volume":"123","author":"Arankalle","year":"2006","journal-title":"Indian. J. Med. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1017\/S0950268804003218","article-title":"Detection of multiple enteric virus strains within a food borne outbreak of gastroenteritis: an indication of the source of contamination","volume":"133","author":"Gallimore","year":"2005","journal-title":"Epidemiol. Infect."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1002\/jmv.21380","article-title":"Molecular characterization of rotaviruses, noroviruses, sapoviruses, and adenoviruses in patients with acute gastroenteritis in Thailand","volume":"81","author":"Kittigul","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1017\/S0022172400062902","article-title":"An epidemic of acute diarrhoea in rural southern India associated with echovirus type 11 infection","volume":"95","author":"Patel","year":"1985","journal-title":"J. Hyg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1017\/S0950268803001912","article-title":"An epidemic of rotavirus diarrhoea in Jawhar Taluk, Thane District, Maharashtra, India, December 2000-January 2001","volume":"132","author":"Kelkar","year":"2004","journal-title":"Epidemiol. Infect."},{"key":"ref_37","first-page":"399","article-title":"Occurrence of group B rotavirus infections in the outbreaks of acute gastroenteritis from western India","volume":"134","author":"Chitambar","year":"2011","journal-title":"Ind. J. Med. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1017\/S0950268803001353","article-title":"Large infantile gastroenteritis outbreak in Albania caused by multiple emerging rotavirus genotypes","volume":"131","author":"Villena","year":"2003","journal-title":"Epidemiol. Infect."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1080\/00365540500532134","article-title":"An outbreak of rotavirus infection among adults in an institution for rehabilitation: long-term residence in a closed community as a risk factor for rotavirus illness","volume":"38","author":"Iijima","year":"2006","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1017\/S0950268809991671","article-title":"Mixed viral infections causing acute gastroenteritis in children in a waterborne outbreak","volume":"138","author":"Lappalainen","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1002\/jmv.21151","article-title":"Recombinant norovirus implicated in gastroenteritis outbreaks in Hiroshima prefecture, Japan","volume":"80","author":"Fukuda","year":"2008","journal-title":"J. Med. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1056\/NEJM199307013290103","article-title":"Enteric viruses and diarrhea in HIV-infected patients","volume":"329","author":"Grohmann","year":"1993","journal-title":"N. Engl. J. Med."},{"key":"ref_43","unstructured":"Available online:http:\/\/www.medindia.net\/news\/view_news_main.asp?x=8583."},{"key":"ref_44","unstructured":"Available online:http:\/\/www.dnaindia.com\/mumbai\/report_malaria-dengue-and-now-cholera_1058561."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/9\/3\/895\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:49:23Z","timestamp":1760219363000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/9\/3\/895"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,14]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3]]}},"alternative-id":["ijerph9030895"],"URL":"https:\/\/doi.org\/10.3390\/ijerph9030895","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,14]]}}}