{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:39:58Z","timestamp":1760243998177,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2009,7,3]],"date-time":"2009-07-03T00:00:00Z","timestamp":1246579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 \u00b1 2 nm diameter) and short tails (32 \u00b1 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage\u2019s basic mechanisms and practical applications.<\/jats:p>","DOI":"10.3390\/ijms10072999","type":"journal-article","created":{"date-parts":[[2009,7,3]],"date-time":"2009-07-03T11:29:49Z","timestamp":1246620589000},"page":"2999-3011","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage"],"prefix":"10.3390","volume":"10","author":[{"given":"Cai-Zhong","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Epidemiology, College of Preventive Medicine, Third Military Medical University \/ Gaotanyan road 30, Shapingba district, Chongqing 400038, China"},{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC \/ Liu zi No.5, Changping district, Beijing, 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Yan","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Preventive Medicine, Third Military Medical University \/ Gaotanyan road 30, Shapingba district, Chongqing 400038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Combine Injury, School of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University \/ Gaotanyan road 30, Shapingba district, Chongqing 400038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bu-Yun","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC \/ Liu zi No.5, Changping district, 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Preventive Medicine, Third Military Medical University \/ Gaotanyan road 30, Shapingba district, Chongqing 400038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang-Yu","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Preventive Medicine, Third Military Medical University \/ Gaotanyan road 30, Shapingba district, Chongqing 400038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Ming","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, College of Preventive Medicine, Third Military Medical University \/ Gaotanyan road 30, Shapingba district, Chongqing 400038, 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2009,7,3]]},"reference":[{"key":"ref_1","first-page":"1599","article-title":"Obtention of lysogenic strains of Corynebacterium parvum and Brucella melitensis","volume":"150","author":"Carrere","year":"1956","journal-title":"CR Seances Soc. 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