{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:47:18Z","timestamp":1792730838114,"version":"4.5.0"},"reference-count":58,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2011,8,5]],"date-time":"2011-08-05T00:00:00Z","timestamp":1312502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Potential epidemics of infectious diseases and the constant threat of bioterrorism demand rapid, scalable, and cost-efficient manufacturing of therapeutic proteins. Molecular farming of tobacco plants provides an alternative for the recombinant production of therapeutics. We have developed a transient production platform that uses Agrobacterium infiltration of Nicotiana benthamiana plants to express a novel anthrax receptor decoy protein (immunoadhesin), CMG2-Fc. This chimeric fusion protein, designed to protect against the deadly anthrax toxins, is composed of the von Willebrand factor A (VWA) domain of human capillary morphogenesis 2 (CMG2), an effective anthrax toxin receptor, and the Fc region of human immunoglobulin G (IgG). We evaluated, in N. benthamiana intact plants and detached leaves, the expression of CMG2-Fc under the control of the constitutive CaMV 35S promoter, and the co-expression of CMG2-Fc with nine different viral suppressors of post-transcriptional gene silencing (PTGS): p1, p10, p19, p21, p24, p25, p38, 2b, and HCPro. Overall, transient CMG2-Fc expression was higher on intact plants than detached leaves. Maximum expression was observed with p1 co-expression at 3.5 days post-infiltration (DPI), with a level of 0.56 g CMG2-Fc per kg of leaf fresh weight and 1.5% of the total soluble protein, a ten-fold increase in expression when compared to absence of suppression. Co-expression with the p25 PTGS suppressor also significantly increased the CMG2-Fc expression level after just 3.5 DPI.<\/jats:p>","DOI":"10.3390\/ijms12084975","type":"journal-article","created":{"date-parts":[[2011,8,5]],"date-time":"2011-08-05T12:29:59Z","timestamp":1312547399000},"page":"4975-4990","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Transient Co-Expression of Post-Transcriptional Gene Silencing Suppressors for Increased in Planta Expression of a Recombinant Anthrax Receptor Fusion Protein"],"prefix":"10.3390","volume":"12","author":[{"given":"Lucas","family":"Arzola","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kittipong","family":"Rattanaporn","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Maclean","sequence":"additional","affiliation":[{"name":"Planet Biotechnology Inc., 25571 Clawiter Road, Hayward, CA 94545, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5145-9968","authenticated-orcid":false,"given":"Karen A.","family":"McDonald","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, University of California, Davis, One Shields Ave, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2011,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e15559","DOI":"10.1371\/journal.pone.0015559","article-title":"Preclinical and Clinical Development of Plant-Made Virus-Like Particle Vaccine against Avian H5N1 Influenza","volume":"5","author":"Landry","year":"2010","journal-title":"PLoS One"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"14645","DOI":"10.1073\/pnas.0607089103","article-title":"Monoclonal antibodies from plants: A new speed record","volume":"103","author":"Hiatt","year":"2006","journal-title":"Proc. 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