{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T02:04:26Z","timestamp":1769825066360,"version":"3.49.0"},"reference-count":56,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T00:00:00Z","timestamp":1320624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sustained high concentration of glucose has been verified toxic to \u03b2-cells. Glucose augments Ca2+-stimulated insulin release in pancreatic \u03b2-cells, but chronic high concentration of glucose could induce a sustained level of Ca2+ in \u03b2-cells, which leads to cell apoptosis. However, the mechanism of high glucose-induced \u03b2-cell apoptosis remains unclear. In this study, we use a calcium channel blocker, nifedipine, to investigate whether the inhibition of intracellular Ca2+ concentration could protect \u03b2-cells from chronic high glucose-induced apoptosis. It was found that in a concentration of 33.3 mM, chronic stimulation of glucose could induce INS-1 \u03b2-cells apoptosis at least through the endoplasmic reticulum stress pathway and 10 \u03bcM nifedipine inhibited Ca2+ release to protect \u03b2-cells from high glucose-induced endoplasmic reticulum stress and apoptosis. These results indicated that inhibition of Ca2+ over-accumulation might provide benefit to attenuate islet \u03b2-cell decompensation in a high glucose environment.<\/jats:p>","DOI":"10.3390\/ijms12117569","type":"journal-article","created":{"date-parts":[[2011,11,7]],"date-time":"2011-11-07T11:36:28Z","timestamp":1320665788000},"page":"7569-7580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Nifedipine Protects INS-1 \u03b2-Cell from High Glucose-Induced ER Stress and Apoptosis"],"prefix":"10.3390","volume":"12","author":[{"given":"Yao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Southeast University, No.87 Dingjiaqiao Road, Nanjing, Jiangsu 210009, China"},{"name":"Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Southeast University, No.87 Dingjiaqiao Road, Nanjing, Jiangsu 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Southeast University, No.87 Dingjiaqiao Road, Nanjing, Jiangsu 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Southeast University, No.87 Dingjiaqiao Road, Nanjing, Jiangsu 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2011,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0092-8674(00)81125-3","article-title":"Type 2 diabetes: When insulin secretion fails to compensate for insulin resistance","volume":"92","author":"Kahn","year":"1998","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1530\/eje.0.1490099","article-title":"Beta-cell apoptosis in the pathogenesis of human type 2 diabetes mellitus","volume":"149","author":"Leonardi","year":"2003","journal-title":"Eur. 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