{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T20:41:17Z","timestamp":1778186477560,"version":"3.51.4"},"reference-count":65,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Young leaves of Manihot esculenta Crantz (Euphorbiaceae), Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae) and Pteridium aquilinum (Dennstaedtiaceae) are currently consumed as green vegetables by peoples in sub-Saharan Africa, Latin America, Asia and their migrants living in Western Europe. Sub-Saharan peoples use Manihot, Abelmoschus and Hibiscus also in the folk medicine to alleviate fever and pain, in the treatment of conjunctivitis, rheumatism, hemorrhoid, abscesses, ... The present study investigates the effects of aqueous extracts of those plants on the production of reactive oxygen species (ROS) and the release of myeloperoxidase (MPO) by equine neutrophils activated with phorbol 12-myristate 13-acetate (PMA). The ROS production was measured by lucigenin-enhanced chemiluminescence (CL), and the release of total MPO by an ELISA method. The study also investigates the effect of the extracts on the activity of MPO by studying its nitration activity on tyrosine and by using a new technique called SIEFED (Specific Immunological Extraction Followed by Enzymatic Detection) that allows studying the direct interaction of compounds with the enzyme. In all experiments, the aqueous extracts of the plants developed concentration-dependent inhibitory effects. A moderate heat treatment did not significantly modify the inhibitory capacity of the extracts in comparison to not heated ones. Total polyphenol and flavonoid contents were determined with an HPLC-UV\/DAD analysis and a spectroscopic method using Folin-Ciocalteu reagent. Some polyphenols with well-known antioxidant activities (caffeic acid, chlorogenic acid, hyperoside, rosmarinic acid and rutin) were found in the extracts and may partly explain the inhibitory activities observed. The role of those dietary and medicinal plants in the treatment of ROS-dependent inflammatory diseases could have new considerations for health.<\/jats:p>","DOI":"10.3390\/ijms13010628","type":"journal-article","created":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T11:56:17Z","timestamp":1326110177000},"page":"628-650","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Polyphenol Content and Modulatory Activities of Some Tropical Dietary Plant Extracts on the Oxidant Activities of Neutrophils and Myeloperoxidase"],"prefix":"10.3390","volume":"13","author":[{"given":"Cesar N.","family":"Tsumbu","sequence":"first","affiliation":[{"name":"Interfacultary Centre of Drug Research (CIRM), Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Hospital Avenue 1, B36, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"},{"name":"Centre for Oxygen Research and Development (CORD), Institute of Chemistry B6a, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ginette","family":"Deby-Dupont","sequence":"additional","affiliation":[{"name":"Centre for Oxygen Research and Development (CORD), Institute of Chemistry B6a, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Tits","sequence":"additional","affiliation":[{"name":"Interfacultary Centre of Drug Research (CIRM), Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Hospital Avenue 1, B36, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc","family":"Angenot","sequence":"additional","affiliation":[{"name":"Interfacultary Centre of Drug Research (CIRM), Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Hospital Avenue 1, B36, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Frederich","sequence":"additional","affiliation":[{"name":"Interfacultary Centre of Drug Research (CIRM), Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Hospital Avenue 1, B36, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephane","family":"Kohnen","sequence":"additional","affiliation":[{"name":"Centre for Oxygen Research and Development (CORD), Institute of Chemistry B6a, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ange","family":"Mouithys-Mickalad","sequence":"additional","affiliation":[{"name":"Centre for Oxygen Research and Development (CORD), Institute of Chemistry B6a, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Serteyn","sequence":"additional","affiliation":[{"name":"Centre for Oxygen Research and Development (CORD), Institute of Chemistry B6a, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"},{"name":"Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine, B41, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Franck","sequence":"additional","affiliation":[{"name":"Centre for Oxygen Research and Development (CORD), Institute of Chemistry B6a, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"},{"name":"Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine, B41, University of Li\u00e8ge, Sart Tilman, 4000 Li\u00e8ge 1, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2012,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.micinf.2003.09.008","article-title":"Neutrophil granules and secretory vesicles in inflammation","volume":"5","author":"Faurschou","year":"2003","journal-title":"Microbes Infect"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1007\/s003900050270","article-title":"Neutrophil myeloperoxidase revisited: it\u2019s role in health and disease","volume":"36","author":"Deby","year":"1999","journal-title":"Intensivmed. Notfallmed"},{"key":"ref_3","first-page":"79","article-title":"La my\u00e9loperoxydase des neutrophiles, une enzyme de d\u00e9fense aux capacit\u00e9s oxydantes","volume":"147","author":"Serteyn","year":"2003","journal-title":"Ann. M\u00e9d. V\u00e9t"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1152\/physrev.00031.2007","article-title":"Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production","volume":"88","author":"Powers","year":"2008","journal-title":"Physiol. Rev"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"20597","DOI":"10.1074\/jbc.M000181200","article-title":"Mechanism of reaction of myeloperoxidase with nitrite","volume":"275","author":"Burner","year":"2000","journal-title":"J. Biolog. Chem"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8080","DOI":"10.1021\/jf071741n","article-title":"Resveratrol inhibits the activity of equine neutrophil myeloperoxidase by a direct interaction with the enzyme","volume":"55","author":"Kohnen","year":"2007","journal-title":"J. Agric. Food Chem"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1161\/01.ATV.20.10.2175","article-title":"Modulation of protein kinase activity and gene expression by reactive oxygen species and their role in vascular physiology and pathophysiology","volume":"20","author":"Griendling","year":"2000","journal-title":"Arterioscler. Thromb. Vasc. Biol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.biocel.2006.07.001","article-title":"Free radicals and antioxidants in normal physiological functions and human disease","volume":"39","author":"Valko","year":"2007","journal-title":"Int. J. Biochem. 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Pharmacol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1517\/13543776.2011.584870","article-title":"NADPH oxidase inhibitors: a patent review","volume":"21","author":"Kim","year":"2011","journal-title":"Expert Opin. Ther. Pat"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1002\/ijc.23161","article-title":"Targeting NOX, INOS and COX-2 in inflammatory cells: Chemoprevention using food phytochemicals","volume":"121","author":"Murakami","year":"2007","journal-title":"Int. J. Cancer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/S0955-2863(02)00208-5","article-title":"Flavonoid antioxidants: Chemistry, metabolism and structure\u2013activity relationships","volume":"13","author":"Heim","year":"2002","journal-title":"J. Nutr. Biochem"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/0891-5849(95)02227-9","article-title":"Structure-antioxidant activity relationships of flavonoids and phenolic acids","volume":"20","author":"Miller","year":"1996","journal-title":"Free Radical Biol. Med"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.jnutbio.2006.10.007","article-title":"A review of the interaction among dietary antioxidants and reactive oxygen species","volume":"18","author":"Seifried","year":"2007","journal-title":"J. Nutr. Biochem"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1007\/s11095-008-9661-9","article-title":"Cancer is a preventable disease that requires major lifestyle changes","volume":"25","author":"Anand","year":"2008","journal-title":"Pharm. Res"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"570S","DOI":"10.1093\/ajcn\/78.3.570S","article-title":"Fruits and vegetables in the prevention of cellular oxidative damage","volume":"78","author":"Prior","year":"2003","journal-title":"Am. J. Clin. Nutr"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.3390\/nu2111106","article-title":"Polyphenols and human health: prevention of disease and mechanisms of action","volume":"2","author":"Vauzour","year":"2010","journal-title":"Nutrients"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/S0278-6915(99)00086-1","article-title":"Mechanisms of action of antioxidants as exemplified in vegetables, tomatoes and tea","volume":"3","author":"Weisburger","year":"1999","journal-title":"Food Chem. Toxicol"},{"key":"ref_22","first-page":"305","article-title":"Influence of cassava mosaic virus on cassava leaf-vegetable quality","volume":"66","author":"Almazan","year":"1989","journal-title":"Trop. Agric"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.jfca.2006.02.007","article-title":"Mani\u00e7oba, a quercetin-rich Amazona dish","volume":"19","author":"Kubo","year":"2006","journal-title":"J. Food Compos. Anal"},{"key":"ref_24","first-page":"3","article-title":"Analgesic activity of ethanolic extract of Manihot esculenta Crantz leaves in mice","volume":"30","author":"Miladiyah","year":"2011","journal-title":"Universa Med"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jep.2007.07.023","article-title":"Herbal medicines as diuretics: a review of the scientific evidence","volume":"114","author":"Wright","year":"2007","journal-title":"J. Ethnopharmacol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"662","DOI":"10.5897\/AJB2003.000-1122","article-title":"African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents","volume":"2","author":"Farombi","year":"2003","journal-title":"Afr. J. Biotechnol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.foodchem.2005.07.058","article-title":"Antioxidant activities of aqueous extracts of selected plants","volume":"99","author":"Wong","year":"2006","journal-title":"Food Chem"},{"key":"ref_28","first-page":"1819","article-title":"Anthocyanins from Indian cassava (Manihot esculenta Crantz) and its antioxidant properties","volume":"2","author":"Suresh","year":"2011","journal-title":"Int. J. Pharm. Sci. Res"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1021\/jf035372g","article-title":"Screening of antioxidant activity in edible plant products: Comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay","volume":"52","author":"Katsube","year":"2004","journal-title":"J. Agric. Food Chem"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3923\/rjbsci.2010.310.313","article-title":"Antioxidative activities and phenolic content of extracts from Okra (Abelmoschus esculentus L.)","volume":"5","author":"Khomsug","year":"2010","journal-title":"Res. J. Biologic. Sci"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/j.fct.2009.01.036","article-title":"Chemical composition and the antioxidative properties of Nigerian Okra seed (Abelmoschus esculentus Moench) Flour","volume":"47","author":"Adelakun","year":"2009","journal-title":"Food Chem. Toxicol"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1002\/jsfa.2630","article-title":"Distribution of 127 edible plant species for antioxidant activities by two assays","volume":"86","author":"Yang","year":"2006","journal-title":"J. Sci. Food Agric"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/S0031-9422(97)00623-7","article-title":"Kaempferol-7-O-Rhamnoside-4\u2032-O-glucoside from Pteridium Aquilinum","volume":"47","author":"Imperato","year":"1998","journal-title":"Phytochemistry"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.foodchem.2004.09.019","article-title":"Dihydrocynnamic acids in Pteridium Aquilinum","volume":"93","author":"Mendez","year":"2005","journal-title":"Food Chem"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"818","DOI":"10.3390\/nu3090818","article-title":"Antioxidant and antiradical activities of Manihot esculenta Crantz (Euphorbiaceae) leaves and other selected tropical green vegetables investigated on lipoperoxidation and PMA activated monocytes","volume":"3","author":"Tsumbu","year":"2011","journal-title":"Nutrients"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1097\/00007890-196411000-00001","article-title":"Evaluation of the Trypan blue technique for determination of cell viability","volume":"2","author":"Tenant","year":"1964","journal-title":"Transplantation"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1177\/104063870501700502","article-title":"Development of an enzyme-linked immunosorbent assay for specific neutrophil myeloperoxidase measurement in blood","volume":"17","author":"Franck","year":"2005","journal-title":"J. Vet. Diagn. Invest"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1177\/104063870601800402","article-title":"A specific method for measurement of equine active myeloperoxidase in biological samples and in in vitro tests","volume":"18","author":"Franck","year":"2006","journal-title":"J. Vet. Diagn. Invest"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1172\/JCI112190","article-title":"Activation of the human neutrophil nicotinamide adenine dinucleotide phosphate (NADPH)-Oxidase by protein kinase C","volume":"76","author":"Cox","year":"1985","journal-title":"J. Clinic. Invest"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1055\/s-2003-45141","article-title":"Anti-inflammatory compounds of plant origin. Part I. Action on arachidonic acid pathway, nitric oxide and nuclear factor kappa B (NF-kappa B)","volume":"69","author":"Calixto","year":"2003","journal-title":"Planta Med"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1055\/s-2004-815483","article-title":"Anti-inflammatory compounds of plant origin. Part II. Modulation of pro-inflammatory cytokines, chemokines and adhesion molecules","volume":"70","author":"Calixto","year":"2004","journal-title":"Planta Med"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0009-8981(02)00015-3","article-title":"Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome C reduction","volume":"319","author":"Walrand","year":"2002","journal-title":"Clin. Chim. Acta"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1074\/jbc.273.4.2015","article-title":"Validation of lucigenin (Bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems","volume":"273","author":"Yunbo","year":"1998","journal-title":"J. Biol. Chem"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1016\/0006-2952(89)90309-2","article-title":"Protein kinase C inhibition by plant flavonoids","volume":"38","author":"Ferriola","year":"1989","journal-title":"Biochem. Pharmacol"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1080\/10715760400016139","article-title":"Caffeic acid derivates: In vitro and in vivo anti-inflammatory properties","volume":"38","author":"Dunna","year":"2004","journal-title":"Free Radic. Res"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1142\/S0192415X11008737","article-title":"Anti-inflammatory activity of hyperoside through the suppression of nuclear factor - \u03ba B activation in mouse peritoneal macrophages","volume":"39","author":"Kim","year":"2011","journal-title":"Am. J. Chin. Med"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.rvsc.2009.04.002","article-title":"Method for co-purification of equine neutrophil elastase and myeloperoxidase from a limited blood volume","volume":"87","author":"Serteyn","year":"2009","journal-title":"Res. Vet. Sci"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gach, O., Magne, J., Franck, T., Derochette, S., Deby, G., Serteyn, D., Defraigne, J.O., Lancelotti, P., Legrand, V., and Pierard, L.A. (2011). Clinical significance of active myeloperoxidase in carotid atherosclerotic plaques. Int. J. Cardiol.","DOI":"10.1016\/j.ijcard.2011.07.068"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1152\/ajpcell.00384.2006","article-title":"The actin cytoskeleton regulates exocytosis of all neutrophils subsets","volume":"292","author":"Jog","year":"2007","journal-title":"Am. J. Physiol. Cell Physiol"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3503","DOI":"10.1182\/blood.V89.10.3503","article-title":"Granules of the human neutrophilic polymorphonuclear leukocytes","volume":"89","author":"Borregard","year":"1997","journal-title":"Blood"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1078\/0940-2993-00260","article-title":"Anti-inflammatory effects of rutin on rat paw oedema and on neutrophils chemotaxis and degranulation","volume":"54","author":"Sheloum","year":"2003","journal-title":"Exp. Toxicol. Pathol"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"8747","DOI":"10.1021\/jm1009988","article-title":"Structure-based design, synthesis, and pharmacological evaluation of 3-(aminoalkyl)-5- fluoroindoles as myeloperoxidase inhibitors","volume":"53","author":"Soubhye","year":"2010","journal-title":"J. Med. Chem"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.abb.2009.12.013","article-title":"(\u2212)-Epicatechin enhances the chlorinating activity of human myeloperoxidase","volume":"495","author":"Kirchner","year":"2010","journal-title":"Arch. Biochem. Biophys"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"577","DOI":"10.33549\/physiolres.931086","article-title":"Inhibitory effect of curcuminoids and tetracurcuminoids on equine activated neutrophils and myeloperoxidase activity","volume":"57","author":"Franck","year":"2008","journal-title":"Physiol. Res"},{"key":"ref_55","first-page":"261","article-title":"Modulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity","volume":"28","author":"Boly","year":"2011","journal-title":"Int. J. Mol. Med"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1021\/tx8000835","article-title":"Flavonoids as substrates and inhibitors of myeloperoxidase: Molecular actions of aglycone and metabolites","volume":"21","author":"Shiba","year":"2008","journal-title":"Chem. Res. Toxicol"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.freeradbiomed.2007.09.017","article-title":"Myricitrin as substrate and inhibitor of myeloperoxidase: Implications for the pharmacological effects of flavonoids","volume":"44","author":"Meotti","year":"2008","journal-title":"Free Radic. Biol. Med"},{"key":"ref_58","unstructured":"Hahn, S.K., Tresh, J.M., and Belloti, A.C. (2001). Cassava: Biology, Production and Utilization, CABI Publishing."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1541-4337.2008.00064.x","article-title":"Processing techniques to reduce toxicity and antinutrients of cassava for use as a staple food","volume":"8","author":"Montagnac","year":"2009","journal-title":"Comp. Rev. Food Sci. Food Saf"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3010","DOI":"10.1021\/jf0115589","article-title":"Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity","volume":"50","author":"Dewanto","year":"2002","journal-title":"J. Agric. Food Chem"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1021\/jf049899k","article-title":"Effect of heat treatment on the antioxidant activity of extracts of citrus peels","volume":"52","author":"Jeong","year":"2004","journal-title":"J. Agric. Food Chem"},{"key":"ref_62","first-page":"53","article-title":"Effect of heat treatment on antioxidant activity of some spices","volume":"4","author":"Temitope","year":"2010","journal-title":"Cont. J. Food Sci. Technol"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0034-5288(18)30727-6","article-title":"Rapid, single-step isolation of equine neutrophils on a discontinuous Percoll density gradient","volume":"42","author":"Pycock","year":"1987","journal-title":"Res. Vet. Sci"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0034-5288(96)90112-5","article-title":"Experimental model for the study by chemiluminescence of the activation of isolated equine leucocytes","volume":"61","author":"Benbarek","year":"1996","journal-title":"Res. Vet. Sci"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0076-6879(99)99017-1","article-title":"Analysis of total phenols and other oxidation substrates and antioxidants by average of Folin-Ciocalteu reagent","volume":"299","author":"Singleton","year":"1999","journal-title":"Methods Enzymol"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/13\/1\/628\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:48:25Z","timestamp":1760219305000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/13\/1\/628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,9]]},"references-count":65,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,1]]}},"alternative-id":["ijms13010628"],"URL":"https:\/\/doi.org\/10.3390\/ijms13010628","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,9]]}}}