{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T21:49:29Z","timestamp":1761428969560,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T00:00:00Z","timestamp":1337299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Bromeliads are of great economic importance in flower production; however little information is available with respect to genetic characterization of cultivated bromeliads thus far. In the present study, a selection of cultivated bromeliads was characterized via inter-simple sequence repeat (ISSR) markers with an emphasis on genetic diversity and population structure. Twelve ISSR primers produced 342 bands, of which 287 (~84%) were polymorphic, with polymorphic bands per primer ranging from 17 to 34. The Jaccard\u2019s similarity ranged from 0.08 to 0.89 and averaged ~0.30 for the investigated bromeliads. The Bayesian-based approach, together with the un-weighted paired group method with arithmetic average (UPGMA)-based clustering and the principal coordinate analysis (PCoA), distinctly grouped the bromeliads from Neoregelia, Guzmania, and Vriesea into three separately clusters, well corresponding with their botanical classifications; whereas the bromeliads of Aechmea other than the recently selected hybrids were not well assigned to a cluster. Additionally, ISSR marker was proven efficient for the identification of hybrids and bud sports of cultivated bromeliads. The findings achieved herein will further our knowledge about the genetic variability within cultivated bromeliads and therefore facilitate breeding for new varieties of cultivated bromeliads in future as well.<\/jats:p>","DOI":"10.3390\/ijms13056040","type":"journal-article","created":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T11:26:32Z","timestamp":1337340392000},"page":"6040-6052","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR) Markers"],"prefix":"10.3390","volume":"13","author":[{"given":"Fei","family":"Zhang","sequence":"first","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaying","family":"Ge","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyong","family":"Wang","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinying","family":"Yu","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolan","family":"Shen","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxin","family":"Liu","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojing","family":"Liu","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danqing","family":"Tian","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuquan","family":"Shen","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongming","family":"Yu","sequence":"additional","affiliation":[{"name":"Flower Research and Development Centre, Zhejiang Academy of Agricutural Sciences, Hangzhou 311202, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2012,5,18]]},"reference":[{"unstructured":"Luther, H.E. (2004). An Alphabetical List of Bromeliad Binomials, The Bromeliad Society International, Inc. [9th ed].","key":"ref_1"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3732\/ajb.92.2.337","article-title":"Phylogenetic relationships in subfamily Tillandsioideae (Bromeliaceae) based on DNA sequence data from seven plastid regions","volume":"92","author":"Barfuss","year":"2005","journal-title":"Am. J. Bot"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1007\/s10531-007-9157-7","article-title":"Bromeliaceae diversity and conservation in Minas Gerais state, Brazil","volume":"16","author":"Versieux","year":"2007","journal-title":"Biodivers. Conserv"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1071\/BT99043","article-title":"High genetic diversity in the only known population of Aechmea tuitensis (Bromeliaceae)","volume":"48","author":"Izquierdo","year":"2000","journal-title":"Aust. J. Bot"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.ympev.2010.01.005","article-title":"Phylogenetic estimation of the core Bromeliads with an emphasis on the genus Aechmea (Bromeliaceae)","volume":"55","author":"Sass","year":"2010","journal-title":"Mol. Phylogenet. Evol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/hdy.2008.65","article-title":"Within-population spatial genetic structure in four naturally fragmented species of a neotropical inselberg radiation, Alcantarea imperialis, A. geniculata, A glaziouana, and A. regina (Bromeliaceae)","volume":"101","author":"Lexer","year":"2008","journal-title":"Heredity"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1093\/aob\/mcn226","article-title":"Genetic relationships and variation in reproductive strategies in four closely related bromeliads adapted to neotropical \u201cinselbergs\u201d: Alcantarea glaziouana, A. regina, A. geniculata and A. imperialis (Bromeliaceae)","volume":"103","author":"Martinelli","year":"2009","journal-title":"Ann. Bot"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1093\/aob\/mch171","article-title":"Diversity and genetic structure of the Mexican endemic epiphyte Tillandsia achyrostachys E. Morr. ex Baker var. achyrostachys (Bromeliaceae)","volume":"94","author":"Vovides","year":"2004","journal-title":"Ann. Bot"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/hdy.2009.116","article-title":"Range-wide patterns of nuclear and chloroplast DNA diversity in Vriesea gigantean (Bromeliaceae), a neotropical forest species","volume":"103","author":"Lexer","year":"2009","journal-title":"Heredity"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1095-8339.2010.01088.x","article-title":"Seed dispersal and population structure in Vriesea gigantea, a bromeliad from the Brazilian Atlantic Rainforest","volume":"164","author":"Paggi","year":"2010","journal-title":"Bot. J. Linn. Soc"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4357","DOI":"10.1007\/s10531-005-3741-5","article-title":"Genetic variation in three endangered species of Encholirium (Bromeliaceae) from Cadeia do Espinhaco, Brazil, detected using RAPD Markers","volume":"15","author":"Cavallari","year":"2006","journal-title":"Biodivers. Conserv"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1055\/s-2004-817802","article-title":"A survey of genetic diversity and reproductive biology of Puya raimondii (Bromeliaceae), the endangered queen of the Andes","volume":"6","author":"Sgorbati","year":"2004","journal-title":"Plant Biol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.scienta.2012.03.002","article-title":"Genetic relatedness among Aechmea species and hybrids inferred from AFLP markers and pedigree data","volume":"139","author":"Zhang","year":"2012","journal-title":"Sci. Hortic"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1006\/geno.1994.1151","article-title":"Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification","volume":"20","author":"Zietkiewicz","year":"1994","journal-title":"Genomics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1046\/j.1439-0523.2003.00924.x","article-title":"Genetic maps of RAPD, AFLP and ISSR markers in Ananas bracteatus and A. comosus using the pseudo-testcross strategy","volume":"123","author":"Carlier","year":"2008","journal-title":"Plant Breed"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s11032-010-9543-9","article-title":"A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers","volume":"29","author":"Carlier","year":"2012","journal-title":"Mol. Breed"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4321","DOI":"10.1093\/nar\/8.19.4321","article-title":"Rapid isolation of high molecular weight plant DNA","volume":"8","author":"Murray","year":"1980","journal-title":"Nucleic Acids Res"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1002\/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q","article-title":"Improved silver staining protocols for high sensitivity protein identification using matrix-assisted desorption\/ionization-time of flight analysis","volume":"1","author":"Mortz","year":"2001","journal-title":"Proteomics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1093\/bioinformatics\/bti282","article-title":"PowerMarker: An integrated analysis environment for genetic marker analysis","volume":"21","author":"Liu","year":"2005","journal-title":"Bioinformatics"},{"unstructured":"Rohlf, F.J. (2005). NTSYSpc Numerical Taxonomy and Multivariate Analysis System, version 2.2, Exeter Software.","key":"ref_20"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1099\/00207713-51-3-731","article-title":"Construction and bootstrap of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: Application to trichomonad parasites","volume":"51","author":"Hampl","year":"2001","journal-title":"Int. J. Syst. Evol. Microbiol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1093\/genetics\/155.2.945","article-title":"Inference of population structure using multilocus genotype data","volume":"1555","author":"Pritchard","year":"2000","journal-title":"Genetics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1111\/j.1755-0998.2009.02591.x","article-title":"Inferring weak population structure with the assistance of sample group information","volume":"9","author":"Hubisz","year":"2009","journal-title":"Mol. Ecol. Resour"},{"unstructured":"Pritchard, J.K., and Falus, D. (2009). Documentation for STRUCTURE Software, version 2.3, The University of Chicago Press.","key":"ref_24"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1111\/j.1365-294X.2005.02553.x","article-title":"Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study","volume":"14","author":"Evanno","year":"2005","journal-title":"Mol. Ecol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s10535-010-0021-3","article-title":"Genetic diversity in important members Cucurbitaceae using isozyme, RAPD and ISSR markers","volume":"54","author":"Sikdar","year":"2010","journal-title":"Biol. Plant"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"383","DOI":"10.3390\/ijms13010383","article-title":"Assessment of genetic diversity of Bermudagrass (Cynodon dactylon) using ISSR markers","volume":"13","author":"Farsani","year":"2012","journal-title":"Int. J. Mol. Sci"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1111\/j.1439-0523.2010.01835.x","article-title":"Genetic relationship between cultivated and wild hazelnuts (Corylus avellana L.) collected in northern Spain","volume":"130","author":"Campa","year":"2011","journal-title":"Plant Breed"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s00606-010-0412-0","article-title":"Genetic diversity among endangered Uruguayan populations of Butia Becc. Species based on ISSR","volume":"292","author":"Gaiero","year":"2011","journal-title":"Plant Syst. Evol"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.scienta.2012.04.017","article-title":"Genetic diversity and population structure of cultivated bromeliad accessions assessed by SRAP markers","volume":"141","author":"Zhang","year":"2012","journal-title":"Sci. Hortic"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s11032-010-9418-0","article-title":"Identification of F1 hybrids of artichoke by ISSR markers and morphological analysis","volume":"27","author":"Bianco","year":"2011","journal-title":"Mol. Breed"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/j.1439-0523.2008.01526.x","article-title":"The use of ISSR markers to identify Texas bluegrass interspecific hybrids","volume":"127","author":"Goldman","year":"2008","journal-title":"Plant Breed"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s10681-005-2839-x","article-title":"DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs","volume":"143","author":"Carvalho","year":"2005","journal-title":"Euphytica"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1007\/s11105-011-0302-9","article-title":"Genetic variability of Thermal Nymphaea (Nymphaeaceae) population based on ISSR markers: Implications on relationships, hybridization, and conservation","volume":"29","author":"Poczai","year":"2011","journal-title":"Plant Mol. Biol. Rep"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1007\/s001220050577","article-title":"Identification of closely related cultivars with inter-simple sequence repeat markers","volume":"95","author":"Fang","year":"1997","journal-title":"Theor. Appl. Genet"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1590\/S0102-05362011000400029","article-title":"Characterization of new variety of Chrysanthemum by using ISSR markers","volume":"29","author":"Palai","year":"2011","journal-title":"Hortic. Bras"},{"key":"ref_37","first-page":"84","article-title":"Genetic relationship of grap cultivar by ISSR (inter-simple sequence repeats) markers","volume":"73","author":"Sabir","year":"2008","journal-title":"Eur. J. Hort. Sci"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"861","DOI":"10.2307\/2657038","article-title":"Genetic structure of the saxicole Pitcairnia geyskesii (Bromeliaceae) on inselbergs in French Guiana","volume":"88","author":"Sarthou","year":"2001","journal-title":"Am. J. Bot"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/j.1600-0587.2009.05446.x","article-title":"Genetic structure of the xerophilous bromliad Pitcairnia geyskesii on inselbergs in French Guiana\u2014A test of the forest refuge hypothesis","volume":"33","author":"Leblois","year":"2010","journal-title":"Ecography"},{"unstructured":"Available online: http:\/\/fcbs.org.","key":"ref_40"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1093\/aob\/mcr283","article-title":"Somatic mutations contribute to genotypic diversity in sterile and fertile populations of the threatened shrub, Grevillea rhizomatosa (Proteaceae)","volume":"109","author":"Gross","year":"2012","journal-title":"Ann. Bot"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1007\/s12033-011-9403-9","article-title":"Study of intra-varietal genetic variability in grapevine cultivars by PCR-derived molecular markers and correlations with the geographic origins","volume":"50","author":"Meneghetti","year":"2012","journal-title":"Mol. Biotechnol"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s12033-011-9423-5","article-title":"Inter- and intra-varietal genetic variability in Malvasia cultivars","volume":"50","author":"Meneghetti","year":"2012","journal-title":"Mol. Biotechnol"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/13\/5\/6040\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:50:20Z","timestamp":1760219420000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/13\/5\/6040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,18]]},"references-count":43,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2012,5]]}},"alternative-id":["ijms13056040"],"URL":"https:\/\/doi.org\/10.3390\/ijms13056040","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2012,5,18]]}}}