{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T13:55:36Z","timestamp":1768398936680,"version":"3.49.0"},"reference-count":66,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T00:00:00Z","timestamp":1342656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>The present study investigated the capacity of formulated Berberis vulgaris extract\/\u03b2-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg\/kg\/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL\/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris\/\u03b2-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies.<\/jats:p>","DOI":"10.3390\/ijms13079014","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T14:56:05Z","timestamp":1342709765000},"page":"9014-9034","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Hepatoprotective Effects of Berberis vulgaris L. Extract\/\u03b2 Cyclodextrin on Carbon Tetrachloride\u2013Induced Acute Toxicity in Mice"],"prefix":"10.3390","volume":"13","author":[{"given":"Anca","family":"Hermenean","sequence":"first","affiliation":[{"name":"Department of Histology, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, 1 Feleacului, Arad 310396, Romania"},{"name":"Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Popescu","sequence":"additional","affiliation":[{"name":"Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurel","family":"Ardelean","sequence":"additional","affiliation":[{"name":"Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6273-8540","authenticated-orcid":false,"given":"Miruna","family":"Stan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoleta","family":"Hadaruga","sequence":"additional","affiliation":[{"name":"Food Quality Department, Faculty of Food Processing Technology, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine, 119 Calea Aradului, Timisoara 300645, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciprian-Valentin","family":"Mihali","sequence":"additional","affiliation":[{"name":"Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marieta","family":"Costache","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anca","family":"Dinischiotu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2012,7,19]]},"reference":[{"key":"ref_1","unstructured":"Zakin, D., and Boyer, T.D. (1996). Anatomy of the Normal Liver. Hepatology: A Textbook of Liver Disease, WB Saunders. [3rd ed]."},{"key":"ref_2","unstructured":"Zimmerman, H.J. (1978). Hepatotoxicity: The Adverse Effects of Drugs and other Chemicals on the Liver, Appleton-Century Crofts Publisher."},{"key":"ref_3","first-page":"357","article-title":"The role of the liver in xenobiotic biotransformation. Part I. The role of the liver and its cells and their interactions","volume":"72","author":"Kaminski","year":"2007","journal-title":"Probl. Forensic Sci"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1080\/713611034","article-title":"Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model","volume":"33","author":"Weber","year":"2003","journal-title":"Crit. Rev. Toxicol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1196\/annals.1371.017","article-title":"Toxicoproteomics in liver injury and inflammation","volume":"1076","author":"Merrick","year":"2006","journal-title":"Ann. N. Y. Acad. Sci"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1080\/10590500701569398","article-title":"Postulated carbon tetrachloride mode of action: A review","volume":"25","author":"Manibusan","year":"2007","journal-title":"J. Environ. Sci. Health C"},{"key":"ref_7","first-page":"410","article-title":"A review of plants used in the treatment of liver disease: Part 1","volume":"3","author":"Luper","year":"1998","journal-title":"Altern. Med. Rev"},{"key":"ref_8","first-page":"178","article-title":"A review of plants used in the treatment of liver disease: Part 2","volume":"4","author":"Luper","year":"1999","journal-title":"Altern. Med. Rev"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/jat.1310","article-title":"Beneficial drugs for liver diseases","volume":"28","author":"Murriel","year":"2008","journal-title":"J. Appl. Toxicol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/S0192-0561(96)00047-1","article-title":"Study on the anti-inflamatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids","volume":"18","author":"Ivanovska","year":"1996","journal-title":"Int. J. Immunopharmacol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1023\/B:CONC.0000033929.60336.bb","article-title":"Phytochemical studies of Berberis vulgaris","volume":"40","author":"Saied","year":"2004","journal-title":"Chem. Nat. Compd"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/ptr.2399","article-title":"Pharmacological and therapeutical effects of Berberis vulgaris and its active constituent, berberine","volume":"22","author":"Imanshahidi","year":"2008","journal-title":"Phytother. Res"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/S0367-326X(03)00156-4","article-title":"Antimicrobial activity of aqueolus extracts and of berberine isolates from Berberis heterophylla","volume":"74","author":"Freile","year":"2003","journal-title":"Fitoterapia"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/ptr.1108","article-title":"In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis","volume":"17","author":"Mahady","year":"2003","journal-title":"Phytother. Res"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0304-3835(99)00264-5","article-title":"Inhibitory effect of Coptidis rhizoma and berberine on the proliferation of human esophageal cancer cell lines","volume":"148","author":"Iizuka","year":"2000","journal-title":"Cancer Lett"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.cbi.2008.09.027","article-title":"Effect of berberine on the antioxidant status, ultrastructural modifications and protein bound carbohydrates in azoxymethane-induced colon cancer in rats","volume":"177","author":"Thirupurasundari","year":"2009","journal-title":"Chem. Biol. Interact"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1002\/jcb.22869","article-title":"Berberine induces autophagic cell death and mitochondrial apoptosis in liver cancer cells: The cellular mechanism","volume":"111","author":"Wang","year":"2010","journal-title":"J. Cell. Biochem"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.canlet.2003.09.002","article-title":"The anti-inflamatory potential of berberine in vitro and in vivo","volume":"203","author":"Kuo","year":"2004","journal-title":"Cancer Lett"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/S0024-3205(02)02200-2","article-title":"A comparative study on the anti-inflamatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the root of Turkish Berberis species","volume":"72","author":"Kupeli","year":"2002","journal-title":"Life Sci"},{"key":"ref_20","first-page":"64","article-title":"Berberine: Alkaloid with wide spectrum of pharmacological activities","volume":"3","author":"Singh","year":"2010","journal-title":"J. Nat. Prod"},{"key":"ref_21","first-page":"663","article-title":"Berberine-Induced bile bilirubin secretion in the rat","volume":"35","author":"Hobara","year":"1984","journal-title":"Curr. Ther. Res. Clin. Exp"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1093\/infdis\/155.5.979","article-title":"Randomized controlled trial of berberine sulfate therapy for diarrhea due to enterotoxigenic Escherichia coli and Vibrio cholera","volume":"155","author":"Rabbani","year":"1978","journal-title":"J. Infect. Dis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/S0022-3565(24)39141-4","article-title":"The effect of berberine chloride on experimental cholitis in rats in vivo and in vitro","volume":"294","author":"Zhou","year":"2000","journal-title":"J. Pharmachol. Exp. Ther"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jep.2005.05.019","article-title":"A pharmacological study on Berberis vulgaris fruit extract","volume":"102","author":"Fatehi","year":"2005","journal-title":"J. Ethnopharmacol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1055\/s-2006-957575","article-title":"Mechanism of the aortic relaxation induced by low concentrations of berberine","volume":"64","author":"Wong","year":"1998","journal-title":"Planta Med"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0002-9149(03)00533-2","article-title":"Efficacy and safety of berberine for congestive heart failure secondary to ischemic or idiopathic dilated cardiomyophathy","volume":"92","author":"Zeng","year":"2003","journal-title":"Am. J. Cardiol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1093\/ecam\/nem070","article-title":"Inhibitory Effects of Coptidis rhizoma and Berberine on Cocaine-induced Sensitization","volume":"6","author":"Lee","year":"2009","journal-title":"Evid. Complement. Altern. Med"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1749-8546-5-33","article-title":"Hepatoprotective effects of berberine on carbon tetrachloride-induced acute hepatotoxicity in rats","volume":"5","author":"Feng","year":"2010","journal-title":"Chin. Med"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0367-326X(99)00098-2","article-title":"Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents","volume":"71","author":"Janbaz","year":"2000","journal-title":"Fitoterapia"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1007\/s00204-002-0351-9","article-title":"Inhibitory effect of berberine on tert-butyl hydroperoxide-induced oxidative damage in rat liver","volume":"76","author":"Hwang","year":"2002","journal-title":"Arch. Toxicol"},{"key":"ref_31","first-page":"45","article-title":"The effects of Taraxacum officinale L. and Berberis vulgaris L. root extracts on carbon tetrachloride induced liver toxicity in rats","volume":"9","author":"Fallah","year":"2010","journal-title":"J. Med. Plants"},{"key":"ref_32","first-page":"669","article-title":"Berberis vulgaris extract\/\u03b2 cyclodextrin nanoparticles synthesis and characterization","volume":"61","author":"Hadaruga","year":"2010","journal-title":"Rev. Chim. (Bucharest)"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1002\/ptr.1236","article-title":"The hepatoprotective effects of Limonium sinense against carbon tetrachloride and beta-D-galactosamine intoxication in rats","volume":"17","author":"Chaung","year":"2003","journal-title":"Phytother. Res"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1159\/000157141","article-title":"Mechanism of carbon tetrachloride hepatotoxicity","volume":"8","author":"Clawson","year":"1989","journal-title":"Pathol. Immunopathol. Res"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bhadauria, M (2012). Propolis prevents hepatorenal injury induced by chronic exposure to carbon tetrachloride. Evid. Complement. Altern. Med.","DOI":"10.1155\/2012\/235358"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.fct.2009.12.011","article-title":"Sylimarin, the antioxidant component and Silybum marianum extracts prevent liver damage","volume":"48","author":"Shaker","year":"2010","journal-title":"Food Chem. Toxicol"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Xu, L., Gao, J., Wang, Y., Yu, W., Zhao, X., Yang, X., Zhong, Z., and Qian, Z.M. (2011). Myrica rubra extracts protect the liver from CCl4-induced damage. Evid. Complement. Altern. Med.","DOI":"10.1093\/ecam\/nep196"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0300-483X(03)00058-1","article-title":"Chloroform, carbon tetrachloride and glutathione depletion induce secondary genotoxicity in liver cells via oxidative stress","volume":"187","author":"Beddowes","year":"2003","journal-title":"Toxicology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1080\/01926230290166724","article-title":"Oxidation of biological systems: Oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification","volume":"30","author":"Kohen","year":"2010","journal-title":"Toxicol. Pathol"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"648","DOI":"10.3923\/jbs.2005.648.653","article-title":"Evaluation of phenolic content and total antioxidant activity in Berberis vulgaris fruit extract","volume":"5","author":"Motalleb","year":"2005","journal-title":"J. Biol. Sci"},{"key":"ref_41","first-page":"47","article-title":"Using HPLC to determination the composition and antioxidant activity of Berberis vulgaris","volume":"29","author":"Parichehr","year":"2009","journal-title":"Eur. J. Sci. Res"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1016\/j.fct.2010.05.025","article-title":"Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat","volume":"48","author":"Zovko","year":"2010","journal-title":"Food Chem. Toxicol"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1017\/S0007114509991322","article-title":"Protective effect of apricot (Prunus armeniaca L.) on hepatic steatosis and damage induced by carbon tetrachloride in Wistar rats","volume":"102","author":"Ozturk","year":"2009","journal-title":"Br. J. Nutr"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1354\/vp.37-3-231","article-title":"Reduction of carbon tetrachloride-induced hepatotoxic effects by oral administration of betaine in male Han-Wistar rats: A morphometric histological study","volume":"37","author":"Junnila","year":"2000","journal-title":"Vet. Pathol"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1248\/bpb.32.1533","article-title":"Berberine inhibits hepatic stellate cell proliferation and prevents experimental liver fibrosis","volume":"32","author":"Sun","year":"2009","journal-title":"Biol. Pharm. Bull"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Zhang, Q., Xiao, X., Feng, K., Wang, T., Li, W., Yuan, T., Sun, X., Sun, Q., Xiang, H., and Wang, H (2011). Berberine moderates glucose and lipid metabolism through multipathway mechanism. Evid. Complement. Altern. Med.","DOI":"10.1155\/2011\/924851"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1248\/bpb.31.1169","article-title":"Chronic effects of berberine on blood, liver glucolipid metabolism and liver PPARs expression in diabetic hyperlipidemic rats","volume":"31","author":"Zhou","year":"2008","journal-title":"Biol. Pharm. Bull"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4897","DOI":"10.3748\/wjg.14.4897","article-title":"Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury","volume":"14","author":"Tasci","year":"2008","journal-title":"World Gastroenterol"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1289\/ehp.11922","article-title":"Oxidatively damaged DNA in rats exposed by oral gavage to C60 fullerenes and single-walled carbon nanotubes","volume":"117","author":"Folkmann","year":"2009","journal-title":"Environ. Health Perspect"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S1383-5742(97)00041-0","article-title":"Adduct formation, mutagenesis and nucleotide excision repair of DNA damage produced by reactive oxygen species and lipid peroxidation product","volume":"410","author":"Moller","year":"1998","journal-title":"Mutat. Res"},{"key":"ref_51","first-page":"683","article-title":"Methyl green pyronin stain distinguishes prolifferating from differentiated nonproliferating cell nuclei after acid denaturation of DNA","volume":"34","author":"Iseki","year":"1986","journal-title":"J. Histochem"},{"key":"ref_52","first-page":"272","article-title":"Modification and application of methyl green-pyronin stain after acid denaturation of DNA","volume":"5","author":"Sen","year":"1999","journal-title":"Prog. Anat. Sci"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"6538","DOI":"10.3748\/wjg.v11.i41.6538","article-title":"Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer","volume":"7","author":"Wang","year":"2005","journal-title":"World J. Gastroenterol"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Manna, P., Bhattacharyya, S., Das, J., Ghosh, J., Parames, C., and Sil, C (2011). Phytomedicinal role of Pithecellobium dulce against CCl4-mediated hepatic oxidative impairments and necrotic cell death. Evid. Complement. Altern. Med.","DOI":"10.1093\/ecam\/neq065"},{"key":"ref_55","first-page":"116","article-title":"Ameliorative effect of aqueous leave extract of Ocimum basilicum on CCl4-induced hepatotoxicity and apoptosis in albino rats","volume":"7","author":"Sakr","year":"2011","journal-title":"J. Am. Sci"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6882-11-102","article-title":"Effect of aqueous extract of Tinospora cordifolia on functions of peritoneal macrophages isolated from CCl4 intoxicated male albino mice","volume":"11","author":"Sengupta","year":"2011","journal-title":"BMC Complement. Altern. Med"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1111\/j.1872-034X.2009.00545.x","article-title":"An insight into the protection of rat liver against ischemia\/reperfusion injury by 2-selenium-bridged beta-cyclodextrin","volume":"39","author":"Linn","year":"2009","journal-title":"Hepatol. Res"},{"key":"ref_58","first-page":"229","article-title":"Tumor-specific cytotoxicity and type of cell death induced by beta-cyclodextrin benzaldehyde inclusion compound","volume":"28","author":"Liu","year":"2008","journal-title":"Anticancer Res"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.bcp.2010.06.022","article-title":"Cyclodextrin-complexed cucurmin exhibits anti-inflamatory and antiproliferative activities superior to those of cucurmin through higher cellular uptake","volume":"80","author":"Yadav","year":"2010","journal-title":"Biochem. Pharmacol"},{"key":"ref_60","first-page":"994","article-title":"Biomaterials: Thermal stability of the oleic acid\/\u03b1 and \u03b2 cyclodextrin complexes","volume":"59","author":"Hadaruga","year":"2008","journal-title":"Rev. Chim. (Bucharest)"},{"key":"ref_61","unstructured":"Bergmayer, H.U. (1974). Catalase in Methods of Enzymatic Analysis, Academic Press. [2nd ed]."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","article-title":"Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels","volume":"44","author":"Beauchamp","year":"1971","journal-title":"Anal. Biochem"},{"key":"ref_63","unstructured":"Beutler, E (1974). Red Cell Metabolism: A Manual of Biochemical Methods, Grune & Stratton Inc. [3rd ed]."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1373\/49.4.690","article-title":"Rapid fluorimetric method to detect total plasma malondialdehyde with mild derivatization conditions","volume":"49","author":"Pellegrini","year":"2003","journal-title":"Clin. Chem"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem"},{"key":"ref_66","first-page":"193","article-title":"A sriking correaltion between lethal activity and apoptotic DNA fragmentation of liver in response of D-galactosamine-sintetized mice to a non-lethal amount of lipopolysaccharide","volume":"24","author":"Zhou","year":"2003","journal-title":"Acta Pharmacol. Sin"}],"updated-by":[{"DOI":"10.3390\/ijms27020788","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T00:00:00Z","timestamp":1342656000000}}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/13\/7\/9014\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T18:40:21Z","timestamp":1768329621000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/13\/7\/9014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,19]]},"references-count":66,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2012,7]]}},"alternative-id":["ijms13079014"],"URL":"https:\/\/doi.org\/10.3390\/ijms13079014","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,19]]}}}