{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:53:14Z","timestamp":1773251594311,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T00:00:00Z","timestamp":1358208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a  dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment.<\/jats:p>","DOI":"10.3390\/ijms14011655","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T11:11:19Z","timestamp":1358248279000},"page":"1655-1666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents"],"prefix":"10.3390","volume":"14","author":[{"given":"Myeong-Jin","family":"Goh","sequence":"first","affiliation":[{"name":"Skin Research Institute, Amorepacific R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin 446-729, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hae-Kwang","family":"Lee","sequence":"additional","affiliation":[{"name":"Skin Research Institute, Amorepacific R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin 446-729, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, 1320 Beijing Road (W), Shanghai 200040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De-Yun","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, 1320 Beijing Road (W), Shanghai 200040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Ho","family":"Yeon","sequence":"additional","affiliation":[{"name":"Amorepacific Shanghai R&I Center, 383 Yumin Road, Jiading District, Shanghai 201801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan-Quan","family":"He","sequence":"additional","affiliation":[{"name":"Amorepacific Shanghai R&I Center, 383 Yumin Road, Jiading District, Shanghai 201801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Cheol","family":"Cho","sequence":"additional","affiliation":[{"name":"Skin Research Institute, Amorepacific R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin 446-729, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Na","sequence":"additional","affiliation":[{"name":"Skin Research Institute, Amorepacific R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin 446-729, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1002\/(SICI)1521-1878(199809)20:9<712::AID-BIES4>3.0.CO;2-I","article-title":"Human pigmentation genetics: The difference is only skin deep","volume":"20","author":"Sturm","year":"1998","journal-title":"Bioessays"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1111\/j.1751-1097.2007.00226.x","article-title":"The Protective Role of Melanin Against UV Damage in Human Skin","volume":"84","author":"Brenner","year":"2008","journal-title":"Photochem. 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