{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T08:27:37Z","timestamp":1773476857276,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2013,2,25]],"date-time":"2013-02-25T00:00:00Z","timestamp":1361750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Coxsackievirus B3 (CVB3) is a causative agent of viral myocarditis, meningitis and pancreatitis. CVB3 overcome their host cells by usurping the translation machinery to benefit viral gene expression. This is accomplished through alternative translation initiation in a cap independent manner at the viral internal ribosomal entry site. The 5\u2019 untranslated region (5\u2019UTR) of CVB3 genomic RNA is highly structured. It is the site of multiple RNA-protein and RNA-RNA interactions and it plays a critical role during translation initiation. Similar to the 5\u2019UTR, CVB3 3\u2019 untranslated region (3\u2019UTR) also contains secondary structural elements consisting of three stem-loops followed by a poly (A) tail sequence. Long-range RNA-RNA interactions between 5\u2019 and 3\u2019 ends of some viral genomes have been observed. Because of their dual role in translation and replication, the 5\u2019 and 3\u2019UTRs represent promising candidates for the study of CVB3 cardiovirulence. Taking into account that efficient initiation of mRNA translation depends on a temporally and spatially orchestrated sequence of protein-protein, protein-RNA and RNA-RNA interactions, and that, at present, little is known about RNA-RNA interactions between CVB3 5\u2019 and 3\u2019UTRs, we aimed in the present study, to assess a possible RNA-RNA interaction between 5\u2019 and 3\u2019UTRs during the initiation of translation of a wild-type and a previously characterized mutant (Sabin3-like) CVB3 strains and to investigate the effect of the Sabin3-like mutation on these potential interactions. For this purpose, \u201cElectrophoretic Mobility Shift\u201d assays were carried out. Data obtained did not show any RNA-RNA direct interactions between the 5\u2019- and 3\u2019- ends. Therefore, we can suggest that the possible mechanism by which 3\u2019UTR enhances CVB3 IRES activity may be by bridging the 5\u2019 to the 3\u2019 end through RNA-protein interaction and not through RNA-RNA direct contact. However, these findings need to be confirmed by carrying out further experiments.<\/jats:p>","DOI":"10.3390\/ijms14034525","type":"journal-article","created":{"date-parts":[[2013,2,25]],"date-time":"2013-02-25T10:53:32Z","timestamp":1361789612000},"page":"4525-4544","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Analysis of RNA-RNA Interactions between  5\u2019 and 3\u2019 Untranslated Regions during the Initiation of  Translation of a Cardiovirulent and a Live-Attenuated Coxsackievirus B3 Strains"],"prefix":"10.3390","volume":"14","author":[{"given":"Amira","family":"Souii","sequence":"first","affiliation":[{"name":"Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99-ES27), Facult\u00e9 de Pharmacie de Monastir, Avenue Avicenne, Monastir 5000, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jawhar","family":"Gharbi","sequence":"additional","affiliation":[{"name":"Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99-ES27), Facult\u00e9 de Pharmacie de Monastir, Avenue Avicenne, Monastir 5000, Tunisia"},{"name":"Institut Sup\u00e9rieur de Biotechnologie de Monastir, Universit\u00e9 de Monastir, Avenue Tahar Hadded, BP 74, Monastir 5000, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manel","family":"M'hadheb-Gharbi","sequence":"additional","affiliation":[{"name":"Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives (LR99-ES27), Facult\u00e9 de Pharmacie de Monastir, Avenue Avicenne, Monastir 5000, Tunisia"},{"name":"Institut Sup\u00e9rieur de Biotechnologie de Monastir, Universit\u00e9 de Monastir, Avenue Tahar Hadded, BP 74, Monastir 5000, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,2,25]]},"reference":[{"key":"ref_1","first-page":"31","article-title":"Coxsackie B viruses and human heartn disease","volume":"223","author":"Baboonian","year":"1997","journal-title":"Curr. 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