{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:20:01Z","timestamp":1790097601721,"version":"4.0.1"},"reference-count":49,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2013,5,15]],"date-time":"2013-05-15T00:00:00Z","timestamp":1368576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hydrogen peroxide (H2O2) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H2O2 production caused by salicylic acid (SA), chitosan (QN), and H2O2 elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 (pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and  18 mM H2O2 concentrations, induced an endogenous H2O2 and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H2O2 production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress.<\/jats:p>","DOI":"10.3390\/ijms140510178","type":"journal-article","created":{"date-parts":[[2013,5,15]],"date-time":"2013-05-15T12:29:39Z","timestamp":1368620979000},"page":"10178-10196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":112,"title":["Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan  Foliar Applications"],"prefix":"10.3390","volume":"14","author":[{"given":"Laura","family":"Mej\u00eda-Teniente","sequence":"first","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flor de Dalia","family":"Dur\u00e1n-Flores","sequence":"additional","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Chapa-Oliver","sequence":"additional","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irineo","family":"Torres-Pacheco","sequence":"additional","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Cruz-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Gonz\u00e1lez-Chavira","sequence":"additional","affiliation":[{"name":"Biotechnology Group, National Institute for Forestry, Agriculture and Livestock Research (INIFAP), Celaya-San Miguel de Allende, km 6, CP 38010, Celaya, Guanajuato, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosal\u00eda","family":"Ocampo-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"Guevara-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University,  C.U Cerro de las Campanas, S\/N, colonia Las Campanas, CP 76010, Santiago de Quer\u00e9taro, Quer\u00e9taro, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1093\/jexbot\/53.372.1237","article-title":"Hydrogen peroxide and nitric oxide as signalling molecules in plants","volume":"53","author":"Neill","year":"2002","journal-title":"J. Exp. Bot"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1083\/jcb.200409170","article-title":"Hydrogen peroxide as a signal controlling plant programmed cell death","volume":"168","author":"Gechev","year":"2005","journal-title":"J. Cell Biol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1104\/pp.106.079160","article-title":"Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants","volume":"141","author":"Pitzschke","year":"2006","journal-title":"Plant Physiol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2011.02449.x","article-title":"Reactive oxygen and oxidative stress tolerance in plant pathogenic Pseudomonas","volume":"327","author":"Fones","year":"2011","journal-title":"FEMS Microbiol. Lett"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev.arplant.55.031903.141701","article-title":"Reactive oxygen species: Metabolism, oxidative stress, and signal transduction","volume":"55","author":"Apel","year":"2004","journal-title":"Annu. Rev. Plant Biol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.tplants.2004.08.009","article-title":"Reactive oxygen gene network of plants","volume":"9","author":"Mittler","year":"2004","journal-title":"Trends. Plant Sci"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1365-3040.2011.02323.x","article-title":"A subcellular localization compendium of hydrogen peroxide-induced proteins","volume":"35","author":"Vanderauwera","year":"2012","journal-title":"Plant Cell. Environ"},{"key":"ref_8","first-page":"17","article-title":"Potential for the use of exogenous chemical elicitors in disease and insect pest management of conifer seedling production","volume":"2","author":"Holopainen","year":"2009","journal-title":"Open For. Sci. J"},{"key":"ref_9","first-page":"9155","article-title":"Use of elicitors as an approach for sustainable agriculture","volume":"9","year":"2010","journal-title":"Afr. J. Biotechnol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nri3141","article-title":"How do plants achieve immunity? Defence without specialized immune cells","volume":"12","author":"Spoel","year":"2012","journal-title":"Nat. Rev. Immunol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.postharvbio.2003.09.016","article-title":"Elicitors of induced disease resistance in postharvest horticultural crops: A brief review","volume":"32","author":"Terry","year":"2004","journal-title":"Postharvest Biol. Technol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4203","DOI":"10.3390\/ijms14024203","article-title":"Agriculture and Bioactives: Achieving Both Crop Yield and Phytochemicals","volume":"14","year":"2013","journal-title":"Int. J. Mol. Sci"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1134\/S1062359011050177","article-title":"Involvement of salicylic acid in induction of nematode resistance in plants","volume":"38","author":"Zinovieva","year":"2011","journal-title":"Biol. Bull"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1046\/j.1364-3703.2003.00150.x","article-title":"Pathogen derived elicitors: Searching for receptors in plants","volume":"4","author":"Montesano","year":"2003","journal-title":"Mol. Plant Pathol"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/BF02931186","article-title":"Enhancement of growth and secondary metabolite biosynthesis: Effect of elicitors derived from plants and insects","volume":"10","author":"Jeong","year":"2005","journal-title":"Biotechnol. Bioprocess Eng"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1016\/j.foodchem.2009.01.078","article-title":"The effects of exogenous ethylene and methyl jasmonate on the accumulation of phenolic antioxidants in selected whole and wounded fresh produce","volume":"115","author":"Heredia","year":"2009","journal-title":"Food Chem"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1046\/j.1365-313X.1997.11061187.x","article-title":"Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction","volume":"11","author":"Zhang","year":"1997","journal-title":"Plant J"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1146\/annurev.phyto.050908.135202","article-title":"Salicylic acid, a multifaceted hormone to combat isease","volume":"47","author":"Vlot","year":"2009","journal-title":"Annu. Rev. Phytopathol"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.pmpp.2012.01.005","article-title":"Expression of a germin-like protein gene (CchGLP) from a geminivirus-resistant pepper (Capsicum chinense Jacq.) enhances tolerance to geminivirus infection in transgenic tobacco","volume":"78","year":"2012","journal-title":"Physiol. Mol. Plant Pathol"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1094\/MPMI-06-10-0126","article-title":"Characterization of Geminivirus Resistance in an Accession of Capsicum chinense Jacq","volume":"24","year":"2011","journal-title":"Mol. Plant. Microbe Interact"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.enzmictec.2004.11.002","article-title":"Jasmonic acid elicitation of anthraquinones with some associated enzymic and non-enzymic antioxidant responses in Morinda elliptica","volume":"36","author":"Chong","year":"2005","journal-title":"Enzyme Microb. Tech"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1094\/MPMI-18-0428","article-title":"Salicylic acid-induced resistance to Cucumber mosaic virus in squash and Arabidopsis thaliana: Contrasting mechanisms of induction and antiviral action","volume":"18","author":"Mayers","year":"2005","journal-title":"Mol. Plant Microbe. Interact"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Iseri, O.D., Korpe, D.A., Sahin, F.I., and Haberal, M. (2013). Hydrogen peroxide pretreatment of roots enhanced oxidative stress response of tomato under cold stress. Acta Physiol Plant.","DOI":"10.1007\/s11738-013-1228-7"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1042\/bj3300115","article-title":"Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on gene expression in Arabidopsis suspension cultures","volume":"330","author":"Desikan","year":"1998","journal-title":"Biochem. J"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.jplph.2004.10.003","article-title":"Hydrogen peroxide mediates defence reponses induced by chitosans of different molecular weights in rice","volume":"162","author":"Lin","year":"2005","journal-title":"J. Plant Physiol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1007\/s00344-011-9253-5","article-title":"The regulation of exogenous jasmonic acid on UV-B stress tolerance in Wheat","volume":"31","author":"Liu","year":"2012","journal-title":"J Plant Growth Regul"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1365-3040.2011.02310.x","article-title":"Metabolism of reactive oxygen species and reactive nitrogen species in pepper (Capsicum annuum L.) plants under low temperature stresspce","volume":"35","author":"Airaki","year":"2012","journal-title":"Plant Cell Environ"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mutlu, S., Karadagoglu, O., Atici, O., and Nalbantoglu, B. (2013). Protective role of salicylic acid applied before cold stress on antioxidative system and protein patterns in barley apoplast. Biol. Plant.","DOI":"10.1007\/s10535-013-0322-4"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111","DOI":"10.57182\/jbiopestic.2.1.111-114","article-title":"Induced resistance in bhendi against powdery mildew by foliar application of salicylic acid","volume":"2","author":"Vimala","year":"2009","journal-title":"J. Biopesticides"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.cropro.2005.03.010","article-title":"Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities","volume":"25","year":"2006","journal-title":"Crop Prot"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1271\/bbb.70684","article-title":"Elicitor and catalase activity of conidia suspensions of various strains of Magnaporthe grisea in suspensions-cultured cells of rice","volume":"72","author":"Tanabe","year":"2008","journal-title":"Biosci. Biotechnol. Biochem"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1080\/09583150902752012","article-title":"Induction of H2O2 and related enzymes in tomato roots infected with root knot nematode (M. javanica) by several chemical and microbial elicitors","volume":"19","author":"Sahebani","year":"2009","journal-title":"Biocontrol. Sci. Technol"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4806","DOI":"10.1093\/emboj\/16.16.4806","article-title":"Differential expression of catalase genes in Nicotiana plumbaginifolia (L.)","volume":"16","author":"Willekens","year":"1994","journal-title":"EMBO J"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S1016-8478(23)13224-9","article-title":"Differential expression of three catalase genes in hot pepper (Capsicum annuum L.)","volume":"20","author":"Lee","year":"2005","journal-title":"Mol. Cells"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.enzmictec.2004.08.038","article-title":"O2\u2212 from elicitor-induced oxidative burst is necessary for triggering phenylalanine ammonia-lyase activation and catharanthine synthesis in Catharanthus roseus cell cultures","volume":"36","author":"Xu","year":"2005","journal-title":"Enzyme Microb. Technol"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1023\/A:1026592509060","article-title":"Hypersensitive response-related death","volume":"44","author":"Heath","year":"2000","journal-title":"Plant Mol. Biol"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1104\/pp.107.103325","article-title":"Hydrogen peroxide generation by the pepper extracellular peroxidase CaPO2 activates local and systemic cell death and defense response to bacterial pathogens","volume":"145","author":"Choi","year":"2007","journal-title":"Plant Physiol"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/j.1365-2745.2010.01752.x","article-title":"Elicitation of foliar resistance mechanisms transiently impairs root association with arbuscular mycorrhizal fungi","volume":"99","author":"Wyatt","year":"2011","journal-title":"J. Ecol"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1016\/j.jprot.2011.05.016","article-title":"Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins","volume":"74","author":"Huang","year":"2011","journal-title":"J. Proteomics"},{"key":"ref_41","unstructured":"Steiner, A.A. (May, January 29). The universal nutrient solution. Wageningen, The Netherlands."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4161\/psb.4.1.7408","article-title":"Chitosan as a MAMP, searching for a PRR","volume":"4","author":"Iriti","year":"2009","journal-title":"Plant Signal Behav"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s12600-011-0147-7","article-title":"Effect of foliar salicylic acid and methyl jasmonate applications on protection against pill-bugs in lettuce plants (Lactuca sativa)","volume":"39","year":"2011","journal-title":"Phytoparasitica"},{"key":"ref_44","first-page":"6439","article-title":"Chitosan application in maize (Zea mays) to counteract the effects of abiotic stress at seedling level","volume":"10","year":"2011","journal-title":"Afr. J. Biotechnol"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.5897\/AJB08.924","article-title":"In vitro evaluation of interactions between acetone extracts of Garcinia kola seeds and some antibiotics","volume":"7","author":"Sibanda","year":"2008","journal-title":"Afr. J. Biotechnol"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0304-4211(83)90136-0","article-title":"Purification and partial characterization of three genetically defined catalases of maize","volume":"29","author":"Chandlee","year":"1983","journal-title":"Plant Sci. Lett"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem"},{"key":"ref_48","first-page":"103","article-title":"Role of l-phenylalanine ammonia lyase in the induced resistance and susceptibility of potato plants","volume":"41","author":"Gerasimova","year":"2005","journal-title":"Appl. Biochem. Microbiol"},{"key":"ref_49","unstructured":"(2002). JMP, version 5.0.1; Software for dynamic data visualisation and deep analytics, SAS Institute."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/5\/10178\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:46:45Z","timestamp":1760219205000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/5\/10178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,15]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5]]}},"alternative-id":["ijms140510178"],"URL":"https:\/\/doi.org\/10.3390\/ijms140510178","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,15]]}}}