{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T03:47:31Z","timestamp":1775274451609,"version":"3.50.1"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T00:00:00Z","timestamp":1369094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Salmonella Enteritidis (SE) is a major foodborne pathogen in the United States and one of the most frequently reported Salmonella serotypes globally. Eggs are the most common food product associated with SE infections in humans. The pathogen colonizes the intestinal tract in layers, and migrates to reproductive organs systemically. Since adhesion to and invasion of chicken oviduct epithelial cells (COEC) is critical for SE colonization in reproductive tract, reducing these virulence factors could potentially decrease egg yolk contamination. This study investigated the efficacy of sub-inhibitory concentrations of three plant-derived antimicrobials (PDAs), namely carvacrol, thymol and eugenol in reducing SE adhesion to and invasion of COEC, and survival in chicken macrophages. In addition, the effect of PDAs on SE genes critical for oviduct colonization and macrophage survival was determined using real-time quantitative PCR (RT-qPCR). All PDAs significantly reduced SE adhesion to and invasion of COEC (p &lt; 0.001).  The PDAs, except thymol consistently decreased SE survival in macrophages (p &lt; 0.001). RT-qPCR results revealed down-regulation in the expression of genes involved in SE colonization and macrophage survival (p &lt; 0.001). The results indicate that PDAs could potentially be used to control SE colonization in chicken reproductive tract; however,  in vivo studies validating these results are warranted.<\/jats:p>","DOI":"10.3390\/ijms140510608","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T14:22:12Z","timestamp":1369146132000},"page":"10608-10625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Effect of Plant Derived Antimicrobials on Salmonella Enteritidis Adhesion to and Invasion of Primary Chicken Oviduct Epithelial Cells in vitro and Virulence Gene Expression"],"prefix":"10.3390","volume":"14","author":[{"given":"Indu","family":"Upadhyaya","sequence":"first","affiliation":[{"name":"Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhinav","family":"Upadhyay","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anup","family":"Kollanoor-Johny","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Darre","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumar","family":"Venkitanarayanan","sequence":"additional","affiliation":[{"name":"Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,5,21]]},"reference":[{"key":"ref_1","unstructured":"Available online: http:\/\/www.ers.usda.gov\/topics\/animal-products\/poultry-eggs\/background.aspx."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0168-1605(02)00004-1","article-title":"Sensitivity analysis of Salmonella Enteritidis levels in contaminated shell eggs using a biphasic growth model","volume":"75","author":"Latimer","year":"2002","journal-title":"Int. J. Food Microbiol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1093\/ps\/83.12.2071","article-title":"Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs","volume":"83","author":"Bialka","year":"2004","journal-title":"Poult. Sci"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.prevetmed.2007.09.002","article-title":"Salmonella in Belgian laying hens: An identification of risk factors","volume":"83","author":"Namata","year":"2008","journal-title":"Prev. Vet. Med"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6361","DOI":"10.1128\/AEM.00961-09","article-title":"Quantification of horizontal transmission of Salmonella Enterica serovar Enteritidis bacteria in pair-housed groups of laying hens","volume":"75","author":"Thomas","year":"2009","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.3382\/ps.2009-00203","article-title":"Effect of the housing system on shedding and colonization of gut and internal organs of laying hens with Salmonella Enteritidis","volume":"88","author":"Ducatelle","year":"2009","journal-title":"Poult. Sci"},{"key":"ref_7","unstructured":"Available online: http:\/\/www.fda.gov\/ForConsumers\/ConsumerUpdates\/ucm170640.htm."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0378-1135(99)00114-5","article-title":"Colonization of the chicken caecum by afimbriate and aflagellate derivatives of Salmonella Enterica serotype Enteritidis","volume":"69","author":"Woodward","year":"1999","journal-title":"Vet. Microbiol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.3382\/ps.2008-00140","article-title":"Salmonella species and Campylobacter jejuni cecal colonization model in broilers","volume":"87","author":"Stern","year":"2008","journal-title":"Poult. Sci"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1111\/j.1574-6976.2008.00161.x","article-title":"Mechanisms of egg contamination by Salmonella Enteritidis","volume":"33","author":"Gantois","year":"2009","journal-title":"FEMS Microbiol. Rev"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"296","DOI":"10.2307\/1592181","article-title":"Salmonella Enteritidis contamination of eggs from hens inoculated by vaginal, cloacal, and intravenous routes","volume":"41","author":"Miyamoto","year":"1997","journal-title":"Avian Dis"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2307\/1593012","article-title":"Differences among six Salmonella serovars in abilities to colonize reproductive organs and to contaminate eggs in laying hens","volume":"45","author":"Okamura","year":"2001","journal-title":"Avian Dis"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1637\/0005-2086(2007)051[0040:COSROT]2.0.CO;2","article-title":"Colonization of specific regions of the reproductive tract and deposition at different locations inside eggs laid by hens infected with Salmonella Enteritidis or Salmonella Heidelberg","volume":"51","author":"Gast","year":"2007","journal-title":"Avian Dis"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6616","DOI":"10.1128\/AEM.01087-08","article-title":"Salmonella Enterica serovar Enteritidis genes induced during oviduct colonization and egg contamination in laying hens","volume":"74","author":"Gantois","year":"2008","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3382\/ps.0730089","article-title":"Mechanism of transovarian transmission of Salmonella Enteritidis in laying hens","volume":"73","author":"Thiagarajan","year":"1994","journal-title":"Poult. Sci"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5015","DOI":"10.1128\/iai.64.12.5015-5021.1996","article-title":"In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella Enteritidis phage type 8","volume":"64","author":"Thiagarajan","year":"1996","journal-title":"Infect. Immun"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1017\/S0950268800050639","article-title":"A comparison of Salmonella Enteritidis phage types from egg-associated outbreaks and implicated laying flocks","volume":"110","author":"Altekruse","year":"1993","journal-title":"Epidemiol. Infect"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1128\/iai.63.7.2443-2449.1995","article-title":"Salmonella Enteritidis colonization of the reproductive tract and forming and freshly laid eggs of chickens","volume":"63","author":"Keller","year":"1995","journal-title":"Infect Immun"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1128\/AEM.72.2.1055-1064.2006","article-title":"Identification of genes associated with survival of Salmonella enterica serovar Enteritidis in chicken egg albumen","volume":"72","author":"Clavijo","year":"2006","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_20","first-page":"45","article-title":"Occurrence of flavonoid aglycones in medicinal plants","volume":"180","author":"Wollenweber","year":"1988","journal-title":"Prog. Clin. Biol. Res"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0168-1605(95)00055-0","article-title":"Bacteriocins: Modes of action and potentials in food preservation and control of food poisoning","volume":"28","author":"Abee","year":"1995","journal-title":"Int. J. Food Microbiol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.bse.2007.03.012","article-title":"Chemical compositions, antimicrobial and herbicidal effects of essential oils isolated from Turkish Tanacetum aucheranum and Tanacetum chiliophyllum var. chiliophyllum","volume":"35","author":"Salamci","year":"2007","journal-title":"Biochem. Syst. Ecol"},{"key":"ref_23","first-page":"171","article-title":"Inhibition of aflatoxin production and growth of Aspergillus flavus by eugenol and onion and garlic extracts","volume":"96","author":"Bilgrami","year":"1992","journal-title":"Indian J Med Res"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ijfoodmicro.2004.03.022","article-title":"Essential oils: Their antibacterial properties and potential applications in foods\u2014A review","volume":"94","author":"Burt","year":"2004","journal-title":"Int. J. Food Microbiol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.fm.2004.08.006","article-title":"Improvement of shelf life and safety of perishable foods by plant essential oils and smoke antimicrobials","volume":"22","author":"Holley","year":"2005","journal-title":"Food Microbiol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.4315\/0362-028X-65.10.1545","article-title":"Bactericidal activities of plant essential oils and some of there isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica","volume":"65","author":"Friedman","year":"2002","journal-title":"J. Food Prot"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1002\/jsfa.2176","article-title":"Daily consumption of phenolics and total antioxi- dant capacity from fruit and vegetables in the American diet","volume":"85","author":"Chun","year":"2005","journal-title":"J. Sci. Food Agric"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1476-0711-4-20","article-title":"Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori","volume":"4","author":"Ali","year":"2005","journal-title":"Ann. Clin. Microbiol. Antimicrob"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0031-9422(00)97014-6","article-title":"Yield and composition of the essential oil of Thymus serpylloides subsp. serpylloides","volume":"36","author":"Arrebola","year":"1994","journal-title":"Phytochemistry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.fct.2003.08.021","article-title":"The FEMA GRAS assessment of cinnamyl derivatives used as flavor ingredients","volume":"42","author":"Adams","year":"2004","journal-title":"Food Chem. Toxicol"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.fct.2004.12.018","article-title":"The FEMA GRAS assessment of hydroxyl- and alkoxy-substituted benzyl derivatives used as flavor ingredients","volume":"43","author":"Adams","year":"2005","journal-title":"Food Chem. Toxicol"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.tox.2009.12.018","article-title":"Investigation of antioxidant, anti- inflammatory and DNA-protective properties of eugenol in thioacetamide-induced liver injury in rats","volume":"268","author":"Baskaran","year":"2010","journal-title":"Toxicology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"490","DOI":"10.3382\/japr.2008-00051","article-title":"Antibacterial effect of trans- cinnamaldehyde on Salmonella Enteritidis and Campylobacter jejuni in chicken drinking water","volume":"17","author":"Darre","year":"2008","journal-title":"J. Appl. Poult. Res"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3382\/japr.2010-00181","article-title":"Antibacterial effect of trans-cinnamaldehyde, eugenol, thymol and carvacrol against Salmonella Enteritidis and Campylobacter jejuni in vitro","volume":"19","author":"Darre","year":"2010","journal-title":"J. Appl. Poult. Res"},{"key":"ref_35","first-page":"2981","article-title":"Reduction of Salmonella enterica serovar Enteritidis colonization in 20-day-old broiler chickens by the plant-derived compounds trans-cinnamaldehyde and eugenol","volume":"8","author":"Mattson","year":"2012","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1016\/j.juro.2010.11.078","article-title":"Trans-cinnamaldehyde decreases attachment and invasion of uropathogenic Escherichia coli in urinary tract epithelial cells by modulating virulence gene expression","volume":"185","author":"Amalaradjou","year":"2011","journal-title":"J. Urol"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0378-1135(03)00038-5","article-title":"Adhesion of Salmonella enterica serotype Enteritidis isolates to chicken isthmal glandular secretions","volume":"93","author":"Meulemans","year":"2003","journal-title":"Vet. Microbiol"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1136\/vr.133.16.391","article-title":"Bacteriological, serological, histological and immunohistochemical findings in laying hens with naturally acquired Salmonella Enteritidis phage type 4 infection","volume":"133","author":"Hoop","year":"1993","journal-title":"Vet. Rec"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1093\/ps\/83.3.352","article-title":"Tubular glands of the isthmus are the predominant colonization sites of Salmonella Enteritidis in the upper oviduct of laying hens","volume":"83","author":"Pasmans","year":"2004","journal-title":"Poult. Sci"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1093\/ps\/79.11.1571","article-title":"Salmonella penetration of eggshells and proliferation in broiler hatching eggs-a review","volume":"79","author":"Cox","year":"2000","journal-title":"Poult. Sci"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1365-2672.2004.02294.x","article-title":"Colonization of the chicken reproductive tract and egg contamination by Salmonella","volume":"97","author":"Haesebrouck","year":"2004","journal-title":"J. Appl. Microbiol"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ebers, K.L., Zhang, C.Y., Zhang, M.Z., Bailey, R.H., and Zhang, S. (2009). Transcriptional profiling avian beta-defensins in chicken oviduct epithelial cells before and after infection with Salmonella entericaserovar Enteritidis. BMC Microbiol, 9.","DOI":"10.1186\/1471-2180-9-153"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1095\/biolreprod.111.092023","article-title":"Characterization and application of oviductal epithelial cells in vitro in Gallus domesticus","volume":"85","author":"Jung","year":"2011","journal-title":"Biol. Reprod"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0014-5793(94)80517-2","article-title":"Gallinacins: Cysteine- rich antimicrobial peptides of chicken leukocytes","volume":"342","author":"Harwig","year":"1994","journal-title":"FEBS Lett"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1007\/s00251-004-0675-0","article-title":"Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken","volume":"56","author":"Lynn","year":"2004","journal-title":"Immunogenetics"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1471-2164-5-56","article-title":"A genome-wide screen identifies a single beta-defensin gene cluster in the chicken: Implications for the origin and evolution of mammalian defensins","volume":"5","author":"Xiao","year":"2004","journal-title":"BMC Genomics"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.rvsc.2010.10.010","article-title":"Transcriptional profiling of antimicrobial peptides avian \u03b2-defensins in the chicken ovary during sexual maturation and in response to Salmonella Enteritidis infection","volume":"92","author":"Michailidis","year":"2012","journal-title":"Res. Vet. Sci"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"979","DOI":"10.3382\/ps.2007-00283","article-title":"Expression of avian beta defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens","volume":"87","author":"Mageed","year":"2008","journal-title":"Poult. Sci"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1080\/03079450902737771","article-title":"Functions exerted by the virulence-associated type-three secretion systems during Salmonella enterica serovar Enteritidis invasion into and survival within chicken oviduct epithelial cells and macrophages","volume":"38","author":"Li","year":"2009","journal-title":"Avian Pathol"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"17025","DOI":"10.1073\/pnas.252607699","article-title":"Transcriptional modulation of bacterial gene expression by subinhibitory concentrations of antibiotics","volume":"99","author":"Goh","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1099\/jmm.0.45637-0","article-title":"Effect of subinhibitory concentration of piperacillin\/tazobactam on Pseudomonas aeruginosa","volume":"53","author":"Fonseca","year":"2004","journal-title":"J. Med. Microbiol"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1080\/0307945042000220561","article-title":"Effect of type 1 fimbriae of Salmonella enterica serotype Enteritidis on bacteremia and reproductive tract infection in laying hens","volume":"33","author":"Haesebrouck","year":"2004","journal-title":"Avian Pathol"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"45","DOI":"10.2307\/1592442","article-title":"Effect of Inv mutations on Salmonella virulence and colonization in 1-day-old White Leghorn chicks","volume":"41","author":"Porter","year":"1997","journal-title":"Avian Dis"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"6734","DOI":"10.1128\/IAI.71.12.6734-6741.2003","article-title":"Association of Salmonella enterica serovar Enteritidis yafD with resistance to chicken egg albumen","volume":"71","author":"Lu","year":"2003","journal-title":"Infect. Immun"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1863-2378.2008.01195.x","article-title":"The Salmonella Enteritidis lipopolysaccharide biosynthesis gene rfbH is required for survival in egg albumen","volume":"56","author":"Gantois","year":"2009","journal-title":"Zoonoses Public Health"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1089\/fpd.2011.1009","article-title":"Salmonella Enteritidis strains from poultry exhibit differential responses to acid stress, oxidative stress, and survival in the egg albumen","volume":"9","author":"Shah","year":"2012","journal-title":"Foodborne Pathog. Dis"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.vetmic.2007.06.025","article-title":"The Salmonella Pathogenicity Island 2 regulator ssrA promotes reproductive tract but not intestinal colonization in chickens","volume":"126","author":"Bohez","year":"2008","journal-title":"Vet. Microbiol"},{"key":"ref_58","first-page":"3997","article-title":"Periplasmic superoxide dismutase protects Salmonella from products of phagocyte NADPH-oxidase and nitric oxide synthase","volume":"94","author":"Ochsner","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1046\/j.1365-2958.2001.02360.x","article-title":"The Salmonella spvB virulence gene encodes an enzyme that ADP-ribosylates actin and destabilizes the cytoskeleton of eukaryotic cells","volume":"39","author":"Lesnick","year":"2001","journal-title":"Mol. Microbiol"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1046\/j.1365-2958.2000.02064.x","article-title":"The spvB gene-product of the Salmonella enterica virulence plasmid is a mono (ADP-ribosyl) transferase","volume":"37","author":"Otto","year":"2000","journal-title":"Mol. Microbiol"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e5551","DOI":"10.1371\/journal.pone.0005551","article-title":"Characterization of MgtC, a virulence factor of Salmonella enterica serovar Typhi","volume":"4","author":"Retamal","year":"2009","journal-title":"PloS One"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3538","DOI":"10.1099\/mic.0.2007\/009316-0","article-title":"Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat","volume":"153","author":"Townsend","year":"2007","journal-title":"Microbiology"},{"key":"ref_63","unstructured":"Erturk, H.N. (1999). Responses of Superoxide Dismutases to Oxidative Stress in Arabidopsis thaliana. [Ph.D. Thesis, Virginia Polytechnic Institute and State University]."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"722","DOI":"10.4315\/0362-028X-72.4.722","article-title":"Prophylactic supplementation of caprylic acid in feed reduces Salmonella enteritidis colonization in commercial broiler chicks","volume":"72","author":"Baskaran","year":"2009","journal-title":"J. Food Prot"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"6894","DOI":"10.1128\/AEM.00928-06","article-title":"Inactivation of adhesion and invasion of food-borne Listeria monocytogenes by bacteriocin-producing Bifidobacterium strains of human origin","volume":"72","author":"Moroni","year":"2006","journal-title":"Appl. Environ. Microbiol"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1196\/annals.1415.028","article-title":"Identification and characterization of thymosin beta-4 in chicken macrophages using whole cell MALDI-TOF","volume":"1112","author":"Kannan","year":"2007","journal-title":"Ann. N. Y. Acad. Sci"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/5\/10608\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:46:52Z","timestamp":1760219212000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/5\/10608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,21]]},"references-count":66,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5]]}},"alternative-id":["ijms140510608"],"URL":"https:\/\/doi.org\/10.3390\/ijms140510608","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,21]]}}}