{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T04:50:12Z","timestamp":1778043012209,"version":"3.51.4"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T00:00:00Z","timestamp":1377734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The purpose of this study was to investigate the possible protective effect of  N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I\/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I\/R, and I\/R + NAS. The hepatic I\/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.<\/jats:p>","DOI":"10.3390\/ijms140917680","type":"journal-article","created":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T12:45:08Z","timestamp":1377780308000},"page":"17680-17693","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Shuna","family":"Yu","sequence":"first","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zheng","sequence":"additional","affiliation":[{"name":"Departments of Pathology, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengchen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caixing","family":"Shi","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Li","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Du","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiying","family":"Jiang","sequence":"additional","affiliation":[{"name":"Departments of Anatomy, Weifang Medical University, Weifang 261053, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School,  Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.suc.2010.04.003","article-title":"Molecular biology of liver ischemia\/reperfusion injury: Established mechanisms and recent advancements","volume":"90","author":"Klune","year":"2010","journal-title":"Surg. 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