{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:54:21Z","timestamp":1791226461381,"version":"4.1.0"},"reference-count":29,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2013,9,27]],"date-time":"2013-09-27T00:00:00Z","timestamp":1380240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>BTG (B-cell translocation gene) can inhibit cell proliferation, metastasis, and angiogenesis and regulate cell cycle progression and differentiation in a variety of cell types. We aimed to clarify the role of BTG1 in ovarian carcinogenesis and progression. A BTG1-expressing plasmid was transfected into ovarian carcinoma cells and their phenotypes and related proteins were examined. BTG1 mRNA expression was detected in ovarian normal tissue (n = 17), ovarian benign tumors (n = 12), and ovarian carcinoma (n = 64) using real-time RT-PCR. Ectopic BTG1 expression resulted in lower growth rate, high cisplatin sensitivity, G1 arrest, apoptosis, and decreased migration and invasion. Phosphoinositide 3-kinase, protein kinase B, Bcl-xL, survivin, vascular endothelial growth factor, and matrix metalloproteinase-2 mRNA and protein expression was reduced in transfectants as compared to control cells. There was higher expression of BTG1 mRNA in normal tissue than in carcinoma tissue (p = 0.001) and in benign tumors than in carcinoma tissue (p = 0.027). BTG1 mRNA expression in International Federation of Gynecology and Obstetrics (FIGO) stage I\/II ovarian carcinomas was higher than that in FIGO stage III\/IV ovarian carcinomas (p = 0.038). Altered BTG1 expression might play a role in the pathogenesis and progression of ovarian carcinoma by modulating proliferation, migration, invasion, the cell cycle, and apoptosis.<\/jats:p>","DOI":"10.3390\/ijms141019670","type":"journal-article","created":{"date-parts":[[2013,9,27]],"date-time":"2013-09-27T12:42:41Z","timestamp":1380285761000},"page":"19670-19680","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas"],"prefix":"10.3390","volume":"14","author":[{"given":"Yang","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Gynecology, the First Affiliated Hospital of China Medical University,  Shenyang 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Feng","family":"Gou","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Institute of Pathology and Pathophysiology, College of Basic Medicine, China Medical University, Shenyang 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gynecology, the First Affiliated Hospital of China Medical University,  Shenyang 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Takano","sequence":"additional","affiliation":[{"name":"Clinical Cancer Institute, Kanagawa Cancer Center, Yokohama 241-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin-Ling","family":"Xiu","sequence":"additional","affiliation":[{"name":"Department of Gynecology, the First Affiliated Hospital of China Medical University,  Shenyang 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua-Chuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Institute of Pathology and Pathophysiology, College of Basic Medicine, China Medical University, Shenyang 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/978-0-387-68969-2_7","article-title":"Ovarian carcinogenesis: An alternative hypothesis","volume":"622","author":"Piek","year":"2008","journal-title":"Adv. Exp. Med. Biol"},{"key":"ref_2","first-page":"399","article-title":"Advances in the understanding of risk factors for ovarian cancer","volume":"50","author":"Bandera","year":"2005","journal-title":"J. Reprod. Med"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6630","DOI":"10.1038\/sj.onc.1207890","article-title":"Nuclear localization of Tob is important for regulation of its antiproliferative activity","volume":"23","author":"Suzuki","year":"2004","journal-title":"Oncogene"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1002\/jcp.21919","article-title":"The mammalian anti-proliferative BTG\/Tob protein family","volume":"222","author":"Winkler","year":"2010","journal-title":"J. Cell. Physiol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.1038\/sj.onc.1204398","article-title":"Identification of functional domains involved in BTG1 cell localization","volume":"20","author":"Rodier","year":"2001","journal-title":"Oncogene"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"15034","DOI":"10.1074\/jbc.271.25.15034","article-title":"The mammalian immediate-early TIS21 protein and the leukemia-associated BTG1 protein interact with a protein-arginine N-methyltransferase","volume":"271","author":"Lin","year":"1996","journal-title":"J. Biol. Chem"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/gcc.2870030106","article-title":"A chromosome 12 coding region is juxtaposed to the MYC protooncogene locus in a t(8;12)(q24;q22) translocation in a case of B-cell chronic lymphocytic leukemia","volume":"3","author":"Rimokh","year":"1991","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3456","DOI":"10.1002\/pmic.200400968","article-title":"Identification of B-cell translocation gene 1 as a biomarker for monitoring the remission of acute myeloid leukemia","volume":"4","author":"Cho","year":"2004","journal-title":"Proteomics"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1002\/j.1460-2075.1992.tb05213.x","article-title":"BTG1, a member of a new family of antiproliferative genes","volume":"11","author":"Rouault","year":"1992","journal-title":"EMBO J"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1038\/sj.onc.1208373","article-title":"Coactivation of nuclear receptors and myogenic factors induces the major BTG1 influence on muscle differentiation","volume":"24","author":"Busson","year":"2005","journal-title":"Oncogene"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"22563","DOI":"10.1074\/jbc.273.35.22563","article-title":"Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex","volume":"273","author":"Rouault","year":"1998","journal-title":"J. Biol. Chem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.1016\/j.yexcr.2007.03.021","article-title":"Role for Btg1 and Btg2 in growth arrest of WEHI-231 cells through arginine methylation following membrane immunoglobulin engagement","volume":"313","author":"Hata","year":"2007","journal-title":"Exp. Cell Res"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1083\/jcb.200307056","article-title":"FoxO3a regulates erythroid differentiation and induces BTG1, an activator of protein arginine methyl transferase 1","volume":"164","author":"Bakker","year":"2004","journal-title":"J. Cell. Biol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1042\/bj3360471","article-title":"Human carbon catabolite repressor protein (CCR4)-associative factor 1: Cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1","volume":"336","author":"Bogdan","year":"1998","journal-title":"Biochem. J"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7432","DOI":"10.1038\/sj.onc.1203193","article-title":"Tob2, a novel anti-proliferative Tob\/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases","volume":"18","author":"Ikematsu","year":"1999","journal-title":"Oncogene"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1158\/1535-7163.MCT-06-0133","article-title":"B cell translocation gene 1 contributes to antisense Bcl-2-mediated apoptosis in breast cancer cells","volume":"5","author":"Nahta","year":"2006","journal-title":"Mol. Cancer Ther"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1074\/jbc.275.1.147","article-title":"The leukemia-associated protein Btg1 and the p53-regulated protein Btg2 interact with the homeoprotein Hoxb9 and enhance its transcriptional activation","volume":"275","author":"Prevot","year":"2000","journal-title":"J. Biol. Chem"},{"key":"ref_18","first-page":"847","article-title":"Antiproliferative gene BTG1 is highly expressed in apoptotic cells in macrophage-rich areas of advanced lesions in Watanabe heritable hyperlipidemic rabbit and human","volume":"78","author":"Corjay","year":"1998","journal-title":"Lab. Invest"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1006\/excr.1999.4486","article-title":"BTG1: A triiodothyronine target involved in the myogenic influence of the hormone","volume":"249","author":"Rodier","year":"1999","journal-title":"Exp. Cell Res"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.freeradbiomed.2005.07.017","article-title":"Repression by oxidative stress of iNOS and cytokine gene induction in macrophages results from AP-1 and NF-kappaB inhibition mediated by B cell translocation gene-1 activation","volume":"39","author":"Cho","year":"2005","journal-title":"Free Radic. Biol. Med"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3894","DOI":"10.1210\/en.2008-1650","article-title":"The B cell translocation gene (BTG) family in the rat ovary: Hormonal induction, regulation, and impact on cell cycle kinetics","volume":"150","author":"Li","year":"2009","journal-title":"Endocrinology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1002\/jcp.20522","article-title":"Transcriptomic analysis of an in vitro murine model of ovarian carcinoma: Functional similarity to the human disease and identification of prospective tumoral markers and targets","volume":"206","author":"Urzua","year":"2006","journal-title":"J. Cell. Physiol"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e1002533","DOI":"10.1371\/journal.pgen.1002533","article-title":"The origin and nature of tightly clustered BTG1 deletions in precursor B-cell acute lymphoblastic leukemia support a model of multiclonal evolution","volume":"8","author":"Waanders","year":"2012","journal-title":"PLoS Genet"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Deng, B., Zhao, Y., Gou, W., Chen, S., Mao, X., Takano, Y., and Zheng, H (2013). Decreased expression of BTG3 was linked to carcinogenesis, aggressiveness, and prognosis of ovarian carcinoma. Tumour Biol.","DOI":"10.1007\/s13277-013-0811-2"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.canlet.2005.12.027","article-title":"Gene expression in the LNCaP human prostate cancer progression model: Progression associated expression in vitro corresponds to expression changes associated with prostate cancer progression in vivo","volume":"244","author":"Chen","year":"2006","journal-title":"Cancer Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/j.1745-7262.2005.00031.x","article-title":"Change of the cell cycle after flutamide treatment in prostate cancer cells and its molecular mechanism","volume":"7","author":"Wang","year":"2005","journal-title":"Asian J. Androl"},{"key":"ref_27","first-page":"3579","article-title":"Expressions of MMP-2, MMP-9 and VEGF are closely linked to growth, invasion, metastasis and angiogenesis of gastric carcinoma","volume":"26","author":"Zheng","year":"2006","journal-title":"Anticancer Res"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"29","DOI":"10.2174\/157489210789702208","article-title":"Inhibitors of the PI3K\/Akt\/mTOR pathway: New hope for breast cancer patients","volume":"5","author":"Ghayad","year":"2010","journal-title":"Recent Pat. Anticancer Drug Discov"},{"key":"ref_29","first-page":"1271","article-title":"Simultaneous siRNA- mediated knockdown of antiapoptotic BCL2, Bcl-xL, XIAP and survivin in bladder cancer cells","volume":"41","author":"Kunze","year":"2012","journal-title":"Int. J. Oncol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/10\/19670\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:49:34Z","timestamp":1760219374000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/10\/19670"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,27]]},"references-count":29,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2013,10]]}},"alternative-id":["ijms141019670"],"URL":"https:\/\/doi.org\/10.3390\/ijms141019670","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,27]]}}}