{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:33:57Z","timestamp":1760243637393,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T00:00:00Z","timestamp":1384992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The \u03b1-(1,2) fucosyltransferases (Fut1 and Fut2) and \u03b1-(1,3) fucosyltransferases (Fut4, Fut9) are responsible for the synthesis of Lewis X (LeX) and Lewis Y (LeY) conjugated to glycoproteins. We recently reported that these fucosyltransferases were differentially expressed in the reproductive tract of male mouse. Here, we studied the effect of androgen on fucosyltransferase expression through the use of mouse castration models. We found that Fut1 mRNA and Fut4 mRNA were upregulated, while Fut2 mRNA and Fut9 mRNA were downregulated by androgen in the caput epididymis. However, in the vas deferens and prostate, only Fut4 mRNA and Fut2 mRNA were respectively upregulated following exposure to androgen. In the seminal vesicle, all fucosyltransferases, with the exception of Fut9, were upregulated. We identified the androgen receptor binding sites (ARBSs) of Fut2, Fut4 and Fut9 in the caput epididymis. Luciferase assay for these ARBSs is able to provide an indication as to why Fut4 and Fut9 are differently expressed and regulated by androgen, although they catalyze the same \u03b1-(1,3) fucose linkage. Our study showed that androgen could differentially regulate the expression of these fucosyltransferases and provided an insight into the characteristic distribution of each fucosyltransferase responsible for LeX\/LeY biosynthesis in the male reproductive tract.<\/jats:p>","DOI":"10.3390\/ijms141123188","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T15:46:34Z","timestamp":1385048794000},"page":"23188-23202","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Different Effects of Androgen on the Expression of Fut1, Fut2, Fut4 and Fut9 in Male Mouse Reproductive Tract"],"prefix":"10.3390","volume":"14","author":[{"given":"Chun-Mei","family":"Wang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang-Gang","family":"Hu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-Fei","family":"Ru","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-Xin","family":"Yao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu-Bin","family":"Ma","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Hua","family":"Gu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shou-Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuo-Min","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Chuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-Lian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences,  Chinese Academy of Sciences, Shanghai 200031, China"},{"name":"Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41R","DOI":"10.1093\/glycob\/cwg054","article-title":"Fucose: Biosynthesis and biological function in mammals","volume":"13","author":"Becker","year":"2003","journal-title":"Glycobiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"158R","DOI":"10.1093\/glycob\/cwl040","article-title":"Fucosylation in prokaryotes and eukaryotes","volume":"16","author":"Ma","year":"2006","journal-title":"Glycobiology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8336","DOI":"10.1128\/MCB.21.24.8336-8345.2001","article-title":"Deficiency of reproductive tract \u03b1(1,2)fucosylated glycans and normal fertility in mice with targeted deletions of the Fut1 or Fut2 \u03b1(1,2)fucosyltransferase locus","volume":"21","author":"Domino","year":"2001","journal-title":"Mol. Cell. Biol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S1074-7613(01)00166-2","article-title":"The \u03b1(1,3)fucosyltransferases Fuct-iv and Fuct-vii exert collaborative control over selectin-dependent leukocyte recruitment and lymphocyte homing","volume":"15","author":"Homeister","year":"2001","journal-title":"Immunity"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4221","DOI":"10.1128\/MCB.24.10.4221-4228.2004","article-title":"Normal embryonic and germ cell development in mice lacking \u03b1 1,3-fucosyltransferase ix (Fut9) which show disappearance of stage-specific embryonic antigen 1","volume":"24","author":"Kudo","year":"2004","journal-title":"Mol. Cell. Biol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1600-0609.1999.tb01849.x","article-title":"A cloned cd15s-negative variant of hl60 cells is deficient in expression of Fut7 and does not adhere to cytokine-stimulated endothelial cells","volume":"63","author":"Weston","year":"1999","journal-title":"Eur. J. Haematol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4723","DOI":"10.1074\/jbc.M809312200","article-title":"Activity, splice variants, conserved peptide motifs, and phylogeny of two new \u03b11,3-fucosyltransferase families (Fut10 and Fut11)","volume":"284","author":"Mollicone","year":"2009","journal-title":"J. Biol. Chem"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1093\/glycob\/cwq152","article-title":"Biosynthesis and distribution of Lewis X- and Lewis Y-containing glycoproteins in the murine male reproductive system","volume":"21","author":"Wang","year":"2011","journal-title":"Glycobiology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1042\/bj1880667","article-title":"Hormonal regulation of protein synthesis in the rat epididymis. Characterization of androgen-dependent and testicular fluid-dependent proteins","volume":"188","author":"Jones","year":"1980","journal-title":"Biochem. J"},{"key":"ref_10","first-page":"47","article-title":"Testicular regulation of epididymal gene expression","volume":"53","author":"Hinton","year":"1998","journal-title":"J. Reprod. Fertil"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Robaire, B., and Hinton, B.T. (2002). The Epididymis: From Molecules to Clinical Practice, Kluwer Academic\/Plenum Publishers.","DOI":"10.1007\/978-1-4615-0679-9"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1210\/me.2010-0226","article-title":"Research resource: Genome-wide mapping of in vivo androgen receptor binding sites in mouse epididymis","volume":"24","author":"Hu","year":"2010","journal-title":"Mol. Endocrinol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.domaniend.2006.09.003","article-title":"Estrogen and androgen receptor expression in relation to steroid concentrations in the adult boar epididymis","volume":"33","author":"Pearl","year":"2007","journal-title":"Domest. Anim. Endocrinol"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1095\/biolreprod.103.026302","article-title":"Androgen-regulated genes in the murine epididymis","volume":"71","author":"Chauvin","year":"2004","journal-title":"Biol. Reprod"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/0018-506X(77)90038-1","article-title":"A quantitative study of serum testosterone, sex accessory organ growth, and the development of intermale aggression in the mouse","volume":"8","author":"Barkley","year":"1977","journal-title":"Horm. Behav"},{"key":"ref_16","unstructured":"Available online: http:\/\/www.genomatix.de\/index.html."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"711","DOI":"10.2164\/jandrol.110.012914","article-title":"Regional expression of androgen receptor coregulators and androgen action in the mouse epididymis","volume":"32","author":"Sipila","year":"2011","journal-title":"J. Androl"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1507\/endocrj.49.75","article-title":"Castration induces apoptosis in the mouse epididymis during postnatal development","volume":"49","author":"Kuhara","year":"2002","journal-title":"Endocr. J"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1074\/jbc.M109958200","article-title":"Tumor necrosis factor \u03b1 increases the expression of glycosyltransferases and sulfotransferases responsible for the biosynthesis of sialylated and\/or sulfated Lewis X epitopes in the human bronchial mucosa","volume":"277","author":"Delmotte","year":"2002","journal-title":"J. Biol. Chem"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.bbagen.2004.03.006","article-title":"Expression of cell surface Lewis X and Y antigens and Fut4 mRNA is increased in jurkat cells undergoing apoptosis","volume":"1672","author":"Azuma","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1248\/bpb.30.655","article-title":"Increased expression of Lewis X and Y antigens on the cell surface and Fut4 mrna during granzyme b-induced jurkat cell apoptosis","volume":"30","author":"Azuma","year":"2007","journal-title":"Biol. Pharm. Bull"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1210\/en.2002-220915","article-title":"Androgen downregulated and region-specific expression of germ cell nuclear factor in mouse epididymis","volume":"144","author":"Hu","year":"2003","journal-title":"Endocrinology"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/11\/23188\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:50:47Z","timestamp":1760219447000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/14\/11\/23188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,21]]},"references-count":22,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11]]}},"alternative-id":["ijms141123188"],"URL":"https:\/\/doi.org\/10.3390\/ijms141123188","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2013,11,21]]}}}