{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:04:38Z","timestamp":1789574678511,"version":"build-2803163510"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T00:00:00Z","timestamp":1387152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Maternal perinatal nutrition may program offspring metabolic features. Epigenetic regulation is one of the candidate mechanisms that may be affected by maternal dietary methyl donors intake as potential controllers of plasma homocysteine levels. Thirty-two Wistar pregnant rats were randomly assigned into four dietary groups during lactation: control, control supplemented with methyl donors, high-fat-sucrose and  high-fat-sucrose supplemented with methyl donors. Physiological outcomes in the offspring were measured, including hepatic mRNA expression and global DNA methylation after weaning. The newborns whose mothers were fed the obesogenic diet were heavier longer and with a higher adiposity and intrahepatic fat content. Interestingly, increased levels of plasma homocysteine induced by the maternal high-fat-sucrose  dietary intake were prevented in both sexes by maternal methyl donors supplementation. Total hepatic DNA methylation decreased in females due to maternal methyl donors administration, while Dnmt3a hepatic mRNA levels decreased accompanying the  high-fat-sucrose consumption. Furthermore, a negative association between Dnmt3a liver mRNA levels and plasma homocysteine concentrations was found. Maternal  high-fat-sucrose diet during lactation could program offspring obesity features, while methyl donors supplementation prevented the onset of high hyperhomocysteinemia. Maternal dietary intake also affected hepatic DNA methylation metabolism, which could be linked with the regulation of the methionine-homocysteine cycle.<\/jats:p>","DOI":"10.3390\/ijms141224422","type":"journal-article","created":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T15:23:30Z","timestamp":1387207410000},"page":"24422-24437","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Maternal Methyl Donors Supplementation during Lactation Prevents the Hyperhomocysteinemia Induced by a  High-Fat-Sucrose Intake by Dams"],"prefix":"10.3390","volume":"14","author":[{"given":"Paul","family":"Cordero","sequence":"first","affiliation":[{"name":"Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-9916","authenticated-orcid":false,"given":"Fermin","family":"Milagro","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"},{"name":"CIBERobn, Physiopathology of Obesity and Nutrition, Centre of Biomedical Research Network, 29029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Campion","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"},{"name":"CIBERobn, Physiopathology of Obesity and Nutrition, Centre of Biomedical Research Network, 29029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5218-6941","authenticated-orcid":false,"given":"J.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"},{"name":"CIBERobn, Physiopathology of Obesity and Nutrition, Centre of Biomedical Research Network, 29029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1017\/S0029665112000055","article-title":"Interplay of early-life nutritional programming on obesity, inflammation and epigenetic outcomes","volume":"71","author":"Martinez","year":"2012","journal-title":"Proc. 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