{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:55:47Z","timestamp":1791305747718,"version":"4.3.1"},"reference-count":29,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T00:00:00Z","timestamp":1402876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1\u03b2, IL-6 and TNF-\u03b1 as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.<\/jats:p>","DOI":"10.3390\/ijms150610855","type":"journal-article","created":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T11:52:47Z","timestamp":1402919567000},"page":"10855-10867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits"],"prefix":"10.3390","volume":"15","author":[{"given":"Chen","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Perfume and Aroma Technology, Shanghai Institute of Technology, Haiquan Road 100, Shanghai 201418, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meifeng","family":"Tao","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Dongchuan Road 800, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Road 600, Shanghai 200233, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jneumeth.2008.02.018","article-title":"Artificial dural sealant that allows multiple penetrations of implantable brain probes","volume":"171","author":"Jackson","year":"2008","journal-title":"J. Neurosci. Methods"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.jocn.2008.05.021","article-title":"The role of fibroblast growth factor-2 in healing the dura mater after inducing cerebrospinal fluid leakage in rats","volume":"16","author":"Nurata","year":"2009","journal-title":"J. Clin. Neurosci."},{"key":"ref_3","first-page":"209","article-title":"Novel dural closure technique using polyg-lactin acid sheet prevents cerebrospinal fluid leakage after spinal surgery","volume":"57","author":"Sugawara","year":"2005","journal-title":"Neurosurgery"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1039\/CT8864900432","article-title":"On an acetic ferment forms cellulose","volume":"49","author":"Brown","year":"1886","journal-title":"J. Chem. Soc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.carbpol.2006.09.006","article-title":"Structural differences of xylans affect their interaction with cellulose","volume":"69","author":"Kabel","year":"2007","journal-title":"Carbohydr. Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s10570-008-9206-8","article-title":"An estimation of the Young\u2019s modulus of bacterial cellulose filaments","volume":"15","author":"Hsieh","year":"2008","journal-title":"Cellulose"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6360","DOI":"10.1016\/j.apsusc.2005.09.064","article-title":"Surface modification of polyester to produce a bacterial cellulose-based vascular prosthetic device","volume":"252","author":"Charpentier","year":"2006","journal-title":"Appl. Surf. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.biomaterials.2004.02.049","article-title":"Bacterial cellulose as a potential scaffold for tissue engineering of cartilage","volume":"26","author":"Svensson","year":"2005","journal-title":"Biomaterials"},{"key":"ref_9","first-page":"69","article-title":"Multifunctional bacterial cellulose\/chitosan composite materials for medical applications","volume":"12","author":"Ciechanska","year":"2004","journal-title":"Fibers Text East Eur."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1002\/jnr.21740","article-title":"Recombinant arginine deiminase reduces inducible nitric oxide synthase iNOS-mediated neurotoxicity in a coculture of neurons and microglia","volume":"86","author":"Yu","year":"2008","journal-title":"J. Neurosci. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"12013","DOI":"10.1073\/pnas.91.25.12013","article-title":"Phamacological and biochemical demonstration of the role of cycloocygenase-2 in inflammation and pain","volume":"91","author":"Seibert","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/0962935031000097673","article-title":"Dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-\u03baB activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line","volume":"12","author":"Vital","year":"2003","journal-title":"Mediat. Inflamm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1038\/sj.bjp.0705533","article-title":"Signal transduction for inhibition of inducible nitric oxide synthase and cyclooxygenase-2 induction by capsaicin and related analogs in macrophages","volume":"140","author":"Chen","year":"2003","journal-title":"Br. J. Phaimacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.jep.2013.03.055","article-title":"In vivo and in vitro anti-inflammatory activity of Lentinus polychrous extract","volume":"147","author":"Fangkrathok","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3171\/2010.9.JNS091764","article-title":"A transparent artificial dura mater made of silk fibroin as an inhibitor of inflammation in craniotomized rats","volume":"114","author":"Kim","year":"2011","journal-title":"J. Neurosurg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1055\/s-0032-1321506","article-title":"New dried human amniotic membrane is useful as a substitute for dural repair after skull base surgery","volume":"73","author":"Tomita","year":"2012","journal-title":"J. Neurol. Surg. B Skull Base"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kawai, H., Nakagawa, I., Nishimura, F., Motoyama, Y., Park, Y.S., Nakamura, M., Nakase, H., Suzuki, S., and Ikada, Y. (2014). Effectiveness of a new gelatin sealant system for dural closure. Neurol. Res.","DOI":"10.1179\/1743132814Y.0000000342"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.msec.2006.10.002","article-title":"Biomimetic synthesis of hydroxyapatite\/bacterial cellulose nanocomposites for biomedical applications","volume":"27","author":"Wan","year":"2007","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.carres.2007.10.024","article-title":"Cellulose synthesized by Acetobacter xylinum in the presence of multi-walled carbon nanotubes","volume":"343","author":"Yan","year":"2008","journal-title":"Carbohydr. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1227\/01.NEU.0000108640.45371.1A","article-title":"Comparison of deep wound infection rates using a synthetic dural substitute (neuro-patch) or pericranium graft for dural closure: A clinical review of 1 year","volume":"54","author":"Malliti","year":"2004","journal-title":"Neurosurgery"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.biomaterials.2005.05.068","article-title":"Influence of copolymer composition of polylactide implants on cranial bone regeneration","volume":"27","author":"Leiggener","year":"2006","journal-title":"Biomaterials"},{"key":"ref_22","first-page":"17","article-title":"Equine pericardium for dural grafts: Clinical results in 200 patients","volume":"51","author":"Montinaro","year":"2007","journal-title":"J. Neurosurg. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"167","DOI":"10.3171\/PED\/2008\/1\/2\/167","article-title":"Allergic reaction to a bovine dura substitute following spinal cord untethering","volume":"1","author":"Foy","year":"2008","journal-title":"J. Neurosurg. Pediatr."},{"key":"ref_24","first-page":"223","article-title":"Evaluation of epidural scar formation in lumbar spine after TachoComb application\u2014An experimental study","volume":"42","author":"Brzezicki","year":"2008","journal-title":"Neurol. Neurochir. Pol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S1130-1473(06)70316-5","article-title":"Time-sparing and effective procedure for dural closure in the posterior fossa using a vicryl mesh (Ethisorb)","volume":"17","author":"Verheggen","year":"2006","journal-title":"Neurocirugia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1227\/01.NEU.0000089481.87226.F7","article-title":"Toward optimal tissue sealants for neurosurgery: Use of a novel hydrogel sealant in a canine durotomy repair model","volume":"53","author":"Preul","year":"2003","journal-title":"Neurosurgery"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1590\/S0102-86502007000300004","article-title":"Biosynthetic cellulose induces the formation of a neoduramater following pre-natal correction of meningomyelocele in fetal sheep","volume":"22","author":"Valente","year":"2007","journal-title":"Acta Cir. Bras."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3171\/jns.2007.106.1.52","article-title":"Safety and efficacy of a novel polyethylene glycol hydrogel sealant for watertight dural repair","volume":"106","author":"Cosgrove","year":"2007","journal-title":"J. Neurosurg"},{"key":"ref_29","unstructured":"The Ministry of Science and Technology of the People\u2019s Republic of China, Guidance Suggestionsfor the Care and Use of Laboratory Animals [EB\/OL], Available online:http:\/\/www.most.gov.cn\/fggw\/zfwj\/zfwj2006\/200609\/t20060930_54389.htm."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/15\/6\/10855\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:12:30Z","timestamp":1760217150000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/15\/6\/10855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,16]]},"references-count":29,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6]]}},"alternative-id":["ijms150610855"],"URL":"https:\/\/doi.org\/10.3390\/ijms150610855","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,16]]}}}