{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:59:14Z","timestamp":1772647154999,"version":"3.50.1"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2014,5,30]],"date-time":"2014-05-30T00:00:00Z","timestamp":1401408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In many developing countries, proper hygiene is not strictly implemented when animals are slaughtered and meat products become contaminated. Contaminated meat may contain Escherichia coli (E. coli) O157:H7 that could cause diseases in humans if these food products are consumed undercooked. In the present study, a total of 94 confirmed  E. coli O157:H7 isolates were subjected to the enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR) typing to generate genetic fingerprints. The ERIC fragments were resolved by electrophoresis on 2% (w\/v) agarose gels. The presence, absence and intensity of band data were obtained, exported to Microsoft Excel (Microsoft Office 2003) and used to generate a data matrix. The unweighted pair group method with arithmetic mean (UPGMA) and complete linkage algorithms were used to analyze the percentage of similarity and matrix data. Relationships between the various profiles and\/or lanes were expressed as dendrograms. Data from groups of related lanes were compiled and reported on cluster tables. ERIC fragments ranged from one to 15 per isolate, and their sizes varied from 0.25 to 0.771 kb. A large proportion of the isolates produced an ERIC banding pattern with three duplets ranging in sizes from 0.408 to 0.628 kb. Eight major clusters  (I\u2013VIII) were identified. Overall, the remarkable similarities (72% to 91%) between the ERIC profiles for the isolate from animal species and their corresponding food products indicated some form of contamination, which may not exclude those at the level of the abattoirs. These results reveal that ERIC PCR analysis can be reliable in comparing the genetic profiles of E. coli O157:H7 from different sources in the North-West Province of South Africa.<\/jats:p>","DOI":"10.3390\/ijms15069735","type":"journal-article","created":{"date-parts":[[2014,5,30]],"date-time":"2014-05-30T12:00:34Z","timestamp":1401451234000},"page":"9735-9747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Genotypic Characterization of Escherichia coli O157:H7  Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis"],"prefix":"10.3390","volume":"15","author":[{"given":"Collins","family":"Ateba","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, North West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2735, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moses","family":"Mbewe","sequence":"additional","affiliation":[{"name":"Department of Water and Sanitation, University of Limpopo, Turfloop Campus,  Private Bag X1106, Sovenga 0727, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1017\/S0950268801005520","article-title":"Epidemiological investigation of the central Scotland outbreak of Escherichia coli O157 infection, November to December 1996","volume":"126","author":"Cowden","year":"2001","journal-title":"Epidemiol. Infect."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1086\/322598","article-title":"The central Scotland Escherichia coli O157: H7 outbreak: Risk factors for the haemolytic uremic syndrome and death among hospitalized patients","volume":"33","author":"Dundas","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106","DOI":"10.3201\/eid0901.020266","article-title":"Shiga toxin-producing Escherichia coli infections associated with haemolytic uremic syndrome, Italy, 1988\u20132000","volume":"9","author":"Tozzi","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1128\/JCM.42.3.1099-1108.2004","article-title":"Characterisation of Shiga toxin-producing E. coli strains isolated from human patients in Germany over a 3-year period","volume":"42","author":"Beutin","year":"2004","journal-title":"Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"928","DOI":"10.3201\/eid1005.030456","article-title":"Haemolytic uraemic syndrome incidence in New York","volume":"10","author":"Chang","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"590","DOI":"10.3201\/eid1104.040833","article-title":"Childhood haemolytic uraemic syndrome, United Kingdom and Ireland","volume":"11","author":"Lynn","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3085","DOI":"10.1016\/S0043-1354(00)00597-2","article-title":"The occurrence of E. coli O157:H7 in Southern African water sources intended for direct and indirect human consumption","volume":"35","author":"Ehlers","year":"2001","journal-title":"Water Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/CMR.11.1.142","article-title":"Diarrhoeagenic Escherichia coli","volume":"11","author":"Nataro","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"370","DOI":"10.3201\/eid0804.000218","article-title":"A waterborne outbreak of Escherichia coli O157 infections and haemolytic uraemic syndrome: Implications for rural water systems","volume":"8","author":"Olsen","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/S0022-3476(99)70100-9","article-title":"Failure of pre-diarrheal antibiotics to prevent haemolytic uraemic syndrome in serologically proven Escherichia coli O157:H7 gastrointestinal infection","volume":"135","author":"Igarashi","year":"1999","journal-title":"J. Paediat."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"862","DOI":"10.4315\/0362-028X-64.6.862","article-title":"Presence of Escherichia coli O157:H7 in ground beef and ground baby beef meat","volume":"64","author":"Uhitil","year":"2004","journal-title":"Food Protect."},{"key":"ref_12","first-page":"729","article-title":"Virulence factors of Escherichia coli O157 and other Shiga toxin-producing","volume":"88","author":"Law","year":"2000","journal-title":"E. coli. Appl. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1128\/JCM.40.1.271-274.2002","article-title":"Direct detection and characterisation of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA and saa","volume":"40","author":"Paton","year":"2002","journal-title":"Clin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1128\/JCM.36.11.3317-3322.1998","article-title":"Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates","volume":"36","author":"Pierard","year":"1998","journal-title":"Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1128\/AEM.66.3.1205-1208.2000","article-title":"A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons","volume":"66","author":"Schmidt","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1086\/421088","article-title":"A multistate outbreak of Escherichia coli O157:H7 infection linked to consumption of beef tacos at a fast-food restaurant chain","volume":"39","author":"Jay","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.4315\/0362-028X-68.6.1198","article-title":"Outbreak of Escherichia coli O157:H7 infections associated with no intact blade-tenderized frozen steaks sold by door-to-door vendors","volume":"68","author":"Laine","year":"2005","journal-title":"Food Protect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"403","DOI":"10.4315\/0362-028X-68.2.403","article-title":"Prevalence and antibiotic resistance profiles of Escherichia coli O157:H7 in beef products from retail outlets in Gaborone, Botswana","volume":"68","author":"Magwira","year":"2005","journal-title":"Food Protect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"397","DOI":"10.7883\/yoken.JJID.2005.397","article-title":"Mass food poisoning caused by beef offal contaminated by Escherichia coli O157","volume":"58","author":"Maruzumi","year":"2005","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1017\/S0950268800057162","article-title":"Cattle as a possible source of verocytotoxin-producing Escherichia coli O157 in man","volume":"111","author":"Chapman","year":"1993","journal-title":"Epidemiol. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0168-1605(00)00518-3","article-title":"Escherichia coli O157:H7 in faeces from cattle, sheep and pigs in the southwest part of Norway during 1998 and 1999","volume":"65","author":"Johnsen","year":"2001","journal-title":"Int. J. Food Microbiol."},{"key":"ref_22","first-page":"7","article-title":"The prevalence of Escherichia coli O157 strains in cattle, pigs and humans in the North-West Province, South Africa","volume":"104","author":"Ateba","year":"2008","journal-title":"SAJS"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1111\/j.1365-2672.2004.02300.x","article-title":"The prevalence and concentration of Escherichia coli O157 in faeces of cattle from different production systems at slaughter","volume":"97","author":"Fegan","year":"2004","journal-title":"Appl. Microbiol."},{"key":"ref_24","first-page":"25","article-title":"Genomic fingerprinting of bacteria with repetitive sequence-based polymerase chain reaction","volume":"5","author":"Versalovic","year":"1994","journal-title":"Methods Mol. Cell Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/j.1574-6968.2007.00948.x","article-title":"Comparison of five Rep-PCR genomic fingerprinting methods for differentiation of faecal Escherichia coli from humans, poultry and wild birds","volume":"277","author":"Mohapatra","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.resmic.2010.11.008","article-title":"Detection of E. coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the North-West Province, South Africa: Public health implications","volume":"162","author":"Ateba","year":"2011","journal-title":"Res. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1128\/iai.57.4.1290-1298.1989","article-title":"Actin accumulation at sites of bacterial adhesion to tissue culture cells: Basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli","volume":"57","author":"Knutton","year":"1989","journal-title":"Infect. Immun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.1128\/AEM.69.5.2444-2447.2003","article-title":"Concentration and prevalence of Escherichia coli O157 in faeces at slaughter","volume":"69","author":"Omisakin","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.ijfoodmicro.2008.08.011","article-title":"Characterisation of Escherichia coli O157 strains from humans, cattle and pigs in the North-West Province, South Africa","volume":"128","author":"Ateba","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/j.1365-2672.2005.02626.x","article-title":"Analysis of 16S\u201323S intergenic spacer regions of the rRNA operons in Edwardsiella ictaluri and Edwardsiella tarda isolates from fish","volume":"99","author":"Panangala","year":"2005","journal-title":"Appl. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0378-1135(02)00107-4","article-title":"Characterization of Escherichia coli O157 isolates from Danish cattle and human patients by genotyping and presence and variants of virulence genes","volume":"88","author":"Nielsen","year":"2002","journal-title":"Vet. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ijfoodmicro.2004.03.018","article-title":"Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: Prevalence and virulence properties","volume":"96","author":"Meichtri","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_33","unstructured":"Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press. [2nd ed.]."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/15\/6\/9735\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:11:58Z","timestamp":1760217118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/15\/6\/9735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,30]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6]]}},"alternative-id":["ijms15069735"],"URL":"https:\/\/doi.org\/10.3390\/ijms15069735","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,30]]}}}