{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:48:41Z","timestamp":1760888921579,"version":"build-2065373602"},"reference-count":17,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2014,5,30]],"date-time":"2014-05-30T00:00:00Z","timestamp":1401408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.<\/jats:p>","DOI":"10.3390\/ijms15069780","type":"journal-article","created":{"date-parts":[[2014,5,30]],"date-time":"2014-05-30T12:00:34Z","timestamp":1401451234000},"page":"9780-9788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus"],"prefix":"10.3390","volume":"15","author":[{"given":"Feng","family":"Qiu","sequence":"first","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyuan","family":"Cao","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiudong","family":"Su","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Yi","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengli","family":"Bi","sequence":"additional","affiliation":[{"name":"Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Beijing 102206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10139","DOI":"10.1128\/JVI.02598-09","article-title":"Nucleotide variability and translation efficiency of the 5' untranslated region of hepatitis A virus: Update from clinical isolates associated with mild and severe hepatitis","volume":"84","author":"Mackiewicz","year":"2010","journal-title":"J. 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Infect."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"358","DOI":"10.2166\/wh.2013.184","article-title":"Genomic copy concentrations of selected waterborne viruses in a slum environment in Kampala, Uganda","volume":"11","author":"Katukiza","year":"2013","journal-title":"J. Water Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jviromet.2013.03.019","article-title":"Development of a new cell culture-based method and optimized protocol for the detection of enteric viruses","volume":"191","author":"Lee","year":"2013","journal-title":"J. Virol. Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1128\/AEM.71.6.3359-3363.2005","article-title":"Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus","volume":"71","author":"Jothikumar","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1128\/AEM.02660-05","article-title":"Development, evaluation, and standardization of a real-time TaqMan reverse transcription-PCR assay for quantification of hepatitis A virus in clinical and shellfish samples","volume":"72","author":"Costafreda","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jcv.2013.06.038","article-title":"Comparison of real-time RT-PCR assays for hepatitis E virus RNA detection","volume":"58","author":"Mokhtari","year":"2013","journal-title":"J. Clin. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e48125","DOI":"10.1371\/journal.pone.0048125","article-title":"Seroprevalence of fecal-oral transmitted hepatitis A and E virus antibodies in Burkina Faso","volume":"7","author":"Traore","year":"2012","journal-title":"PLoS One"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jviromet.2004.01.025","article-title":"Detection and quantitation of hepatitis E virus in human faeces by real-time quantitative PCR","volume":"118","author":"Orru","year":"2004","journal-title":"J. Virol. Methods"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jviromet.2005.07.004","article-title":"A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus","volume":"131","author":"Jothikumar","year":"2006","journal-title":"J. Virol. 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