{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:19:57Z","timestamp":1790723997113,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2014,7,4]],"date-time":"2014-07-04T00:00:00Z","timestamp":1404432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The main effects of three different irrigation regimes, i.e., sustained deficit irrigation (SDI), regulated deficit irrigation (RDI) and non-irrigated (NI), on seed traits namely proanthocyanidins (PAs) were evaluated in the wine grape cultivar Aragonez (syn. Tempranillo) grown in Alentejo (Portugal) over two growing seasons. Results showed that while the number of seeds per berry was not affected by water availability, seed fresh weight differed among treatments, the NI treatment exhibiting the lowest values.  The biosynthetic pathway of flavanols appeared to be modified by the irrigation treatment, and several genes responsible for PA synthesis were up-regulated in the most stressed seeds (RDI and NI). However, this effect had no impact on PA content, suggesting the influence of other factors such as oxidation and\/or degradation of PAs at late stages of maturation in grape seeds. The seeds\u2019 non-enzymatic antioxidant capacities (oxygen radical absorbance capacity (ORAC) and hydroxyl radical adverting capacity (HORAC)) were modulated by water deficit and correlated well with PA content. The impact of irrigation strategy on PA biosynthesis, content, and anti-radical activity during seed ripening is discussed in the context of increasing interest in the role of PAs in the color and taste of wine, and the potential health benefits relating to their antioxidant capacity.<\/jats:p>","DOI":"10.3390\/ijms150711862","type":"journal-article","created":{"date-parts":[[2014,7,4]],"date-time":"2014-07-04T10:37:36Z","timestamp":1404470256000},"page":"11862-11877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Proanthocyanidin Accumulation and Biosynthesis Are Modulated by the Irrigation Regime in Tempranillo Seeds"],"prefix":"10.3390","volume":"15","author":[{"given":"Tania","family":"Genebra","sequence":"first","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquen","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Francisco","sequence":"additional","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Pinto-Marijuan","sequence":"additional","affiliation":[{"name":"Departament of Biologia Vegetal , Facultat de Biologia, Barcelona University,  Barcelona 6450-8028, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricard","family":"Brossa","sequence":"additional","affiliation":[{"name":"Departament of Biologia Vegetal , Facultat de Biologia, Barcelona University,  Barcelona 6450-8028, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Serra","sequence":"additional","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"},{"name":"IBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, Oeiras 2781-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina","family":"Duarte","sequence":"additional","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"},{"name":"IBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, Oeiras 2781-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Chaves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4115-4781","authenticated-orcid":false,"given":"Olfa","family":"Zarrouk","sequence":"additional","affiliation":[{"name":"Instituto de Tecnol\u00f3gia Quimica e Biologica, University Nova of Lisbon,  Oeiras 2780-901, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1469-8137.2004.01217.x","article-title":"Proanthocyanidins\u2014A final frontier in flavonoid research?","volume":"165","author":"Dixon","year":"2005","journal-title":"New Phytol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.3390\/molecules13123007","article-title":"Chemical synthesis of proanthocyanidins in vitro and their reactions in aging wines","volume":"13","author":"He","year":"2008","journal-title":"Molecules"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1749-6632.2002.tb02903.x","article-title":"Wine phenolics","volume":"957","author":"Waterhouse","year":"2002","journal-title":"Ann. 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