{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T02:33:58Z","timestamp":1780108438988,"version":"3.54.0"},"reference-count":24,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T00:00:00Z","timestamp":1404691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Neurological degeneration can occur after compression of the spinal cord. It is widely accepted that spinal cord compression leads to ischemic lesions and ultimately neurological dysfunction due to a narrowed spinal canal. Therefore, an in-depth understanding of the pathogenesis of spinal cord compression injury is required to help develop effective clinical interventions. In the present study, we propose a new method of quantitative 3D micro-CT to observe microvascular events in a chronic spinal cord compression rat model. A total of 36 rats were divided into two groups: sham control group (n = 12) and compressive spinal cord injury group (n = 24). Rats were scarified at four weeks after surgery. In each group, CD34 micro-vessel immunohistochemical staining was performed in half of the animals, while micro-CT scanning was performed in the other half. Microvessel density (MVD) was measured after immunohistochemical staining, while the vascular index (VI) was measured in 3D micro-CT. In comparison with sham control, abnormal somatosensory evoked potentials (SEP) can be seen in all 24 cases of the compression group, and VI shows the amount of microvessels reduced consistently and significantly (p &lt; 0.01). A significant correlation is also found between MVD and VI  (r = 0.95, p &lt; 0.01). These data suggest that quantitative 3D micro-CT is a sensitive and promising tool for investigating microvascular changes during chronic compressive spinal cord injury.<\/jats:p>","DOI":"10.3390\/ijms150712061","type":"journal-article","created":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T11:00:56Z","timestamp":1404730856000},"page":"12061-12073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression"],"prefix":"10.3390","volume":"15","author":[{"given":"Hou-Qing","family":"Long","sequence":"first","affiliation":[{"name":"Department of Spine Surgery, the First Affiliated Hospital of Sun Yat-Sen University,  Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Han","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery, the First Affiliated Hospital of Sun Yat-Sen University,  Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, the First Affiliated Hospital of Sun Yat-Sen University,  Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Lin","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital of Sun Yat-Sen University,  Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Hui","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery, the First Affiliated Hospital of Sun Yat-Sen University,  Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology, Li Kai Shing Faculty of Medicine,  the University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1097\/BRS.0b013e318221b5af","article-title":"Evaluation of treatment effectiveness for the herniated cervical disc: A systematic review","volume":"37","author":"Gebremariam","year":"2012","journal-title":"Spine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1097\/BRS.0b013e318241ad33","article-title":"Ossification of the posterior longitudinal ligament of the cervical spine: Etiology and natural history","volume":"37","author":"Matsunaga","year":"2012","journal-title":"Spine"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3109\/02688697.2012.724120","article-title":"Review of spinal neuroectodermal tumor","volume":"27","author":"Patnaik","year":"2013","journal-title":"Br. J. Neurosurg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1177\/1073858412467377","article-title":"Cervical spondylotic myelopathy: The clinical phenomenon and the current pathobiology of an increasingly prevalent and devastating disorder","volume":"19","author":"Karadimas","year":"2013","journal-title":"Neuroscientist"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"190S","DOI":"10.1016\/j.spinee.2006.04.024","article-title":"Pathophysiology of cervical myelopathy","volume":"6","author":"Baptiste","year":"2006","journal-title":"Spine"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1007\/BF00796525","article-title":"Colored microspheres reveal interarterial microvascular anastomoses in canine myocardium","volume":"87","author":"Cicutti","year":"1992","journal-title":"Basic Res. Cardiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"367","DOI":"10.4103\/0973-029X.125200","article-title":"VEGF and CD 34: A correlation between tumor angiogenesis and microvessel density\u2014An immunohistochemicalstudy","volume":"17","author":"Kukreja","year":"2013","journal-title":"J. Oral Maxillofac. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1097\/00007632-197909000-00001","article-title":"Effect of mannitol on the traumatized spinal cord. Microangiography, blood flow patterns, and electrophysiology","volume":"4","author":"Reed","year":"1979","journal-title":"Spine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s12194-012-0163-4","article-title":"Visualization of mouse spinal cord microscopic structures by use of ex vivo quantitative micro-CT images","volume":"6","author":"Saito","year":"2013","journal-title":"Radiol. Phys. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.neulet.2009.04.033","article-title":"In vivo micro-CT imaging of rat brain glioma: A comparison with 3T MRI and histology","volume":"458","author":"Engelhorn","year":"2009","journal-title":"Neurosci. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1016\/j.clinph.2010.12.051","article-title":"Somatosensory-evoked potentials as an indicator for the extent of ultrastructural damage of the spinal cord after chronic compressive injuries in a rat model","volume":"122","author":"Hu","year":"2011","journal-title":"Clin. Neurophysiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.neulet.2013.04.024","article-title":"Is the speed of chronic compression an important factor for chronic spinal cord injury rat model?","volume":"545","author":"Long","year":"2013","journal-title":"Neurosci. Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1111\/j.1582-4934.2011.01489.x","article-title":"Erythropoietin attenuates the sequels of is chaemic spinal cord injury with enhanced recruitment of CD34+ cells in mice","volume":"16","author":"Hirano","year":"2012","journal-title":"J. Cell Mol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1097\/BRS.0b013e3181eaf33d","article-title":"Altered blood flow distribution in the rat spinal cord underchronic compression","volume":"36","author":"Kurokawa","year":"2011","journal-title":"Spine"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1097\/00007632-200108150-00006","article-title":"The Torg-Pavlov ratio in cervical spondylotic myelopathy: A comparative study between patients with cervical spondylotic myelopathy and a nonspondylotic, nonmyelopathic population","volume":"26","author":"Yue","year":"2001","journal-title":"Spine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3171\/jns.1968.28.6.0565","article-title":"Experimental study of ischemic damage to the cervical spinal cord","volume":"28","author":"Shimomura","year":"1968","journal-title":"J. Neurosurg."},{"key":"ref_17","first-page":"149","article-title":"Human dental age estimation by cone beam computed tomography\u2014An in vitro study","volume":"31","author":"Fritsch","year":"2013","journal-title":"J. Forensic. Odontostomatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.placenta.2008.08.017","article-title":"Quantitative 3D micro-CT imaging of the human feto-placental vasculature in intrauterine growth restriction","volume":"29","author":"Langheinrich","year":"2008","journal-title":"Placenta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.vascn.2009.03.004","article-title":"High-throughput micro-computed tomography imaging as a method to evaluate rat and rabbit fetal skeletal abnormalities for developmental toxicity studies","volume":"59","author":"Winkelmann","year":"2009","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1093\/brain\/76.1.70","article-title":"The pathology of spinal cord lesions and their relation to the clinical features in protrusion of cervical intervertebraldiscs: A report of four cases","volume":"76","author":"Mair","year":"1953","journal-title":"Brain"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.nbd.2013.02.013","article-title":"A novel experimental model of cervical spondylotic myelopathy (CSM) to facilitate translational research","volume":"54","author":"Karadimas","year":"2013","journal-title":"Neurobiol. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.jneumeth.2011.10.018","article-title":"Three-dimensional alteration of microvasculature in a rat model of traumatic spinal cord injury","volume":"204","author":"Hu","year":"2012","journal-title":"J. Neurosci. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Karadimas, S.K., Gatzounis, G., and Fehlings, M.G. (2014). Pathobiology of cervical spondylotic myelopathy. Eur. Spine J.","DOI":"10.1007\/s00586-014-3264-4"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/978-1-4613-1259-8_14","article-title":"Angiogenesis in breast cancer","volume":"83","author":"Weidner","year":"1996","journal-title":"Cancer Treat. Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/15\/7\/12061\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:13:20Z","timestamp":1760217200000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/15\/7\/12061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,7]]},"references-count":24,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2014,7]]}},"alternative-id":["ijms150712061"],"URL":"https:\/\/doi.org\/10.3390\/ijms150712061","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,7]]}}}