{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:39:36Z","timestamp":1762101576225,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T00:00:00Z","timestamp":1404777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Grant of National Science Foundation of China","award":["81171469"],"award-info":[{"award-number":["81171469"]}]},{"name":"Program Project of Technology Department of Guangdong Province of China","award":["2011B031800136"],"award-info":[{"award-number":["2011B031800136"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human pheochromocytoma cells, which are demonstrated to contain and release met-enkephalin and norepinephrine, may be a promising resource for cell therapy in cancer-induced intractable pain. Intrathecal injection of alginate-poly (l) lysine-alginate (APA) microencapsulated human pheochromocytoma cells leads to antinociceptive effect in a rat model of bone cancer pain, and this effect was blocked by opioid antagonist naloxone and alpha 2-adrenergic antagonist rauwolscine. Neurochemical changes of cerebrospinal fluid are in accordance with the analgesic responses. Taken together, these data support that human pheochromocytoma cell implant-induced antinociception was mediated by met-enkephalin and norepinephrine secreted from the cell implants and acting at spinal receptors. Spinal implantation of microencapsulated human pheochromocytoma cells may provide an alternative approach for the therapy of chronic intractable pain.<\/jats:p>","DOI":"10.3390\/ijms150712135","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T11:15:26Z","timestamp":1404818126000},"page":"12135-12148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiao","family":"Li","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University,  Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0304-3959(91)90163-R","article-title":"Pain causes in 200 patients referred to a multidisciplinary cancer pain clinic","volume":"45","author":"Banning","year":"1991","journal-title":"Pain"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1002\/(SICI)1097-0142(19971015)80:8+<1588::AID-CNCR9>3.0.CO;2-G","article-title":"Skeletal complications of malignancy","volume":"80","author":"Coleman","year":"1997","journal-title":"Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6243","DOI":"10.1158\/1078-0432.CCR-06-0931","article-title":"Clinical features of metastatic bone disease and risk of skeletal morbidity","volume":"12","author":"Coleman","year":"2006","journal-title":"Clin. 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